公开(公告)号：WO2021223029A1

公开(公告)日：2021-11-11

申请号：PCT/CA2021/050633

申请日：2021-05-06

Applicant: UNIVERSITY HEALTH NETWORK

Inventor： LUPIEN, Mathieu ,  MADANI TONEKABONI, Seyed Ali ,  NADORP, Bettina ,  QAMRA, Aditi

IPC: C12Q1/6809 ,  C12Q1/6886 ,  G16B20/00 ,  G16B30/00

Abstract: The present disclosure relates to a method for determining the presence of a cancer stem cell in a cancer sample comprising at least one tumor cell. Chromatin accessibility data is obtained from the cancer sample. A DNA repeat profile is generated based on an enrichment factor of DNA repeats in one or more portions of the chromatin accessibility data. The DNA repeat profile is compared to one or more corresponding reference DNA repeat profiles associated with a cancer stem cell. The reference profile comprises a reference enrichment factor of one or more corresponding portions of the chromatin accessibility data. When the DNA repeat profile for the one or more portions of the chromatin accessibility data matches the one or more first corresponding reference DNA repeat profiles to within a predetermined threshold, a cancer stem cell status is assigned based on the one or more first corresponding reference DNA repeat profiles.