公开(公告)号：WO1994024296A2

公开(公告)日：1994-10-27

申请号：PCT/CA1994000201

申请日：1994-04-19

Applicant: UNIVERSITY OF SASKATCHEWAN

Inventor： UNIVERSITY OF SASKATCHEWAN ,  ZAMB, Timothy ,  LIANG, Xiaoping ,  RABUIK, Lorne, A.

IPC: C12N15/86

CPC classification number: C12N15/86 ,  A61K39/00 ,  A61K39/12 ,  A61K39/265 ,  A61K2039/5254 ,  A61K2039/552 ,  C07K14/005 ,  C12N15/85 ,  C12N2710/16722 ,  C12N2710/16734 ,  C12N2710/16743 ,  C12N2800/60 ,  C12N2830/00 ,  C12N2830/60

Abstract: Vaccines compositions comprising a pharmaceutically acceptable vehicle and a mutant bovine herpesvirus 1 (BHV-1) wherein (i) at least one non-essential gene of wild-type BHV-1 is deleted and optionally replaced with a gene selected from the group consisting of a foreign gene, a gene encoding a BHV-1 immunogen and a mutant analog of a gene encoding a BHV-1 immunogen; and wherein (ii) the replacement gene is optionally under the control of a promoter; and further wherein (iii) at least one essential gene of wild-type BHV-1 is optionally mutated are provided. Vaccines compositions comprising a pharmaceutically acceptable vehicle and a bovine herpesvirus 1 (BHV-1) wherein (i) at least one non-essential gene is deleted or (ii) an essential gene is mutated are also provided. In addition, related vectors, DNA constructs, a diagnostic assay and methods for treating and preventing BHV-1 infection and other bacterial and viral pathogens of cattle are provided.

Abstract translation: 包含药学上可接受的载体和突变体牛疱疹病毒1（BHV-1）的疫苗组合物，其中（i）野生型BHV-1的至少一种非必需基因被缺失，并且任选地被选自以下的基因替换： 外源基因，编码BHV-1免疫原的基因和编码BHV-1免疫原的基因的突变体类似物; 并且其中（ii）所述置换基因任选地在启动子的控制下; 并且其中（iii）提供野生型BHV-1的至少一种必需基因被任选突变。 还提供了包含药学上可接受的载体和牛疱疹病毒1（BHV-1）的疫苗组合物，其中（i）至少一个非必需基因被缺失或（ii）必需基因被突变。 此外，还提供了相关载体，DNA构建体，诊断测定法和用于治疗和预防牛的BHV-1感染和其他细菌和病毒病原体的方法。