公开(公告)号：WO1997046664A1

公开(公告)日：1997-12-11

申请号：PCT/US1997009875

申请日：1997-06-06

Applicant: UNIVERSITY OF WASHINGTON

Inventor： UNIVERSITY OF WASHINGTON ,  SNOW, Alan ,  FUKUCHI, Ken-Ichiro ,  HASSELL, John

IPC: C12N05/00

CPC classification number: C12N15/8509 ,  A01K67/0275 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/0306 ,  A01K2267/0312 ,  A01K2267/0318 ,  A01K2267/0356 ,  A01K2267/0362 ,  A61K49/0008 ,  C07K14/4711 ,  C12N2800/108 ,  C12N2830/008

Abstract: The invention provides a transgenic non-human animal expressing a perlecan encoding transgene. Also provided is a double-transgenic non-human animal expressing a perlecan and an amyloid encoding transgene. A method of screening for a compound which alters the rate or extent of amyloid deposition is additionally provided. The method consists of: (a) constructing a perlecan transgenic animal; (b) administering an effective amount of a test compound to said perlecan transgenic animal; and (c) determining whether said test compound alters the extent or rate of amyloid deposition. Finally, the invention provides a method of screening for a compound which alters the rate or extent of amyloid deposition. The method consists of: (a) constructing a perlecan/amyloid double-transgenic animal; (b) administering an effective amount of a test compound to said perlecan/amyloid double-transgenic animal; and (c) determining whether said test compound alters the extent or rate of amyloid deposition.

Abstract translation: 本发明提供了表达编码转基因的全基因组的转基因非人动物。 还提供了表达perlecan和编码转基因淀粉样蛋白的双重转基因非人动物。 另外提供筛选改变淀粉样蛋白沉积速度或程度的化合物的方法。 该方法包括：（a）构建一个全基因组转基因动物; （b）向所述perlecan转基因动物施用有效量的测试化合物; 和（c）确定所述测试化合物是否改变淀粉样蛋白沉积的程度或速率。 最后，本发明提供了筛选改变淀粉样蛋白沉积速度或程度的化合物的方法。 该方法包括：（a）构建一个perlecan /淀粉样蛋白双转基因动物; （b）向所述perlecan /淀粉样蛋白双转基因动物施用有效量的测试化合物; 和（c）确定所述测试化合物是否改变淀粉样蛋白沉积的程度或速率。

公开(公告)号：EP0937137A1

公开(公告)日：1999-08-25

申请号：EP97933136.0

申请日：1997-06-06

Applicant: University of Washington

Inventor： SNOW, Alan ,  FUKUCHI, Ken-Ichiro ,  HASSELL, John

IPC: C12N15 ,  A01K67 ,  A61K49 ,  C07K14 ,  C12N5

CPC classification number: C12N15/8509 ,  A01K67/0275 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/0306 ,  A01K2267/0312 ,  A01K2267/0318 ,  A01K2267/0356 ,  A01K2267/0362 ,  A61K49/0008 ,  C07K14/4711 ,  C12N2800/108 ,  C12N2830/008

Abstract: The invention provides a transgenic non-human animal expressing a perlecan encoding transgene. Also provided is a double-transgenic non-human animal expressing a perlecan and an amyloid encoding transgene. A method of screening for a compound which alters the rate or extent of amyloid deposition is additionally provided. The method consists of: (a) constructing a perlecan transgenic animal; (b) administering an effective amount of a test compound to said perlecan transgenic animal; and (c) determining whether said test compound alters the extent or rate of amyloid deposition. Finally, the invention provides a method of screening for a compound which alters the rate or extent of amyloid deposition. The method consists of: (a) constructing a perlecan/amyloid double-transgenic animal; (b) administering an effective amount of a test compound to said perlecan/amyloid double-transgenic animal; and (c) determining whether said test compound alters the extent or rate of amyloid deposition.