公开(公告)号：EP2281011B1

公开(公告)日：2013-02-27

申请号：EP09747510.7

申请日：2009-05-13

Applicant: University of Washington ,  PhaseRx, Inc.

Inventor： STAYTON, Patrick, S. ,  HOFFMAN, Allan, S. ,  CONVERTINE, Anthony, J. ,  BENOIT, Danielle ,  DUVALL, Craig, L. ,  JOHNSON, Paul ,  GALL, Anna

IPC: C08F293/00 ,  C12N15/87 ,  A61K48/00

CPC classification number: C08F293/005 ,  A61K31/713 ,  A61K47/32 ,  A61K47/58 ,  C07K2319/10 ,  C08F293/00 ,  C12N15/111 ,  C12N15/113 ,  C12N15/87 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/351 ,  C12N2320/32

Abstract: Described herein are copolymers, and methods of making and utilizing such copolymers. Such copolymers have at least two blocks: a first block that has at least one unit that is hydrophilic at physiologic pH, and a second block that has hydrophobic groups. This second block further has at least one unit with a group that is anionic at about physiologic pH. The described copolymers are disruptive of a cellular membrane including an extracellular membrane, an intracellular membrane, a vesicle, an organelle, an endosome, a liposome, or a red blood cell. Preferably, in certain instances, the copolymer disrupts the membrane and enters the intracellular environment. In specific examples, the copolymer is endosomolytic.

公开(公告)号：EP2285853A2

公开(公告)日：2011-02-23

申请号：EP09747512.3

申请日：2009-05-13

Applicant: University of Washington ,  Phaserx, Inc.

Inventor： STAYTON, Patrick, S. ,  HOFFMAN, Allan, S. ,  CONVERTINE, Anthony, J. ,  DUVALL, Craig, L. ,  BENOIT, Danielle ,  OVERELL, Robert ,  JOHNSON, Paul ,  GALL, Anna ,  PRIEVE, Mary ,  PASCHAL, Amber ,  DIAB, Charbel ,  DE, Priyadarsi

IPC: C08F299/00 ,  C08F299/02 ,  C08F299/04 ,  C08F293/00 ,  C12N15/87 ,  A61K48/00

CPC classification number: A61K31/713 ,  A61K9/1075 ,  A61K47/549 ,  A61K47/58 ,  A61K47/60 ,  A61K47/6907 ,  A61K48/0041 ,  C08F290/062 ,  C08F293/005 ,  C12N15/111 ,  C12N15/87 ,  C12N2320/32 ,  Y02A50/463

Abstract: Provided herein are micellic assemblies comprising a plurality of copolymers. In certain instances, micellic assemblies provided herein are pH sensitive particles.

Abstract translation: 本文提供了包含多种共聚物的胶束装配体。 在某些情况下，本文提供的胶束装配体是pH敏感颗粒。

公开(公告)号：EP0815121A1

公开(公告)日：1998-01-07

申请号：EP96905419.0

申请日：1996-02-02

Applicant: University of Washington

Inventor： STAYTON, Patrick, S.

IPC: G01N33 ,  C07K1 ,  C07K14 ,  C12N15 ,  C12P21 ,  C12R1

CPC classification number: C07K1/22 ,  C07K14/36

Abstract: Streptavidin tetramers have at least one monomer containing an amino acid modification that produces a reduced binding affinity for biotin, a modified off-rate, a modified on-rate, or an altered binding enthalpy. Polynucleotides encoding the modified monomers are also provided. The modified streptavidin and chimeric streptavidin molecules are useful in methods of bioseparations and cell sorting, imaging, drug delivery, and diagnostics.

公开(公告)号：WO2012064381A1

公开(公告)日：2012-05-18

申请号：PCT/US2011/035256

申请日：2011-05-04

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION ,  STAYTON, Patrick, S. ,  LAI, Jriuan ,  NEHILLA, Barrett, J. ,  SRINIVASAN, Selvi

Inventor： STAYTON, Patrick, S. ,  LAI, Jriuan ,  NEHILLA, Barrett, J. ,  SRINIVASAN, Selvi

IPC: G01N33/58 ,  G01N33/543 ,  G01N33/553 ,  B82Y25/00 ,  C12N15/10

CPC classification number: B01D21/0009 ,  B82Y25/00 ,  G01N27/745 ,  G01N33/54366 ,  G01N2001/4088 ,  Y02A50/53 ,  Y02A50/58 ,  Y10T436/255

Abstract: The present disclosure utilizes the aggregation of stimuli-responsive polymers to isolate a diagnostic target (e.g., an antigen) from a solution using magnetophoresis. Isolating the diagnostic target provides a route to identify the presence of the diagnostic target in the solution. For example, utilized is a two-component assay (eg sandwich assay) comprising a magnetic nanoparticle functionalized with a stimuli-responsive polymer, and a capture molecule attached to a second stimuli-responsive polymer.

Abstract translation: 本公开使用刺激响应聚合物的聚集来使用磁电泳从溶液中分离诊断靶（例如抗原）。 隔离诊断目标提供了一种路径，用于识别解决方案中诊断目标的存在。 例如，所使用的是包含用刺激响应性聚合物官能化的磁性纳米颗粒和连接到第二刺激响应性聚合物的捕获分子的双组分测定（例如夹心测定）。

公开(公告)号：WO2010021770A1

公开(公告)日：2010-02-25

申请号：PCT/US2009/043859

申请日：2009-05-13

Applicant: UNIVERSITY OF WASHINGTON ,  PHASERX, INC. ,  JOHNSON, Paul ,  STAYTON, Patrick, S. ,  HOFFMAN, Allan, S. ,  OVERELL, Robert ,  GALL, Anna ,  PRIEVE, Mary ,  PASCHAL, Amber ,  DIAB, Charbel ,  DE, Priyadarsi

Inventor： JOHNSON, Paul ,  STAYTON, Patrick, S. ,  HOFFMAN, Allan, S. ,  OVERELL, Robert ,  GALL, Anna ,  PRIEVE, Mary ,  PASCHAL, Amber ,  DIAB, Charbel ,  DE, Priyadarsi

IPC: A61K9/127 ,  A61K48/00 ,  A61K47/34 ,  A61K31/7088 ,  A61K31/7105

CPC classification number: A61K9/0019 ,  A61K9/1075 ,  A61K47/58 ,  C08L53/00 ,  C12N15/88 ,  C08L2666/24

Abstract: Compositions comprising a heterogeneous polymeric micelle and an agent (e.g., a polynucleotide) associated with the micelle are disclosed, together with methods for intracellular delivery of such agent.

Abstract translation: 公开了包含非均相聚合物胶束和与胶束相关联的试剂（例如，多核苷酸）的组合物，以及用于细胞内递送该试剂的方法。

公开(公告)号：WO2018165198A1

公开(公告)日：2018-09-13

申请号：PCT/US2018/021206

申请日：2018-03-06

Applicant: UNIVERSITY OF WASHINGTON ,  SEATTLE CHILDREN'S RESEARCH INSTITUTE ,  COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION

Inventor： STAYTON, Patrick, S. ,  CONVERTINE, Anthony ,  DAS, Debobrato ,  SON, Hye-Nam ,  SRINIVASAN, Selvi ,  MONTGOMERY, Katherine ,  BLUMENTHAL, Ian ,  CRANE, Courtney ,  JENSEN, Michael ,  MATTHAEI, James ,  CHIEFARI, John ,  DANIAL, Maarten ,  HUANG, Fei ,  MACDONALD, James ,  POSTMA, Almar ,  TURNER, Kathleen

IPC: C12N5/10 ,  C12N15/67 ,  A61K47/50 ,  C07K16/28

Abstract: Provided herein are engineered cells and methods for engineering cells to deliver a therapeutic agent, e.g., a small molecule, peptide or other drug, to a cell or tissue to be treated.

公开(公告)号：WO2012171035A1

公开(公告)日：2012-12-13

申请号：PCT/US2012/041962

申请日：2012-06-11

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION ,  STAYTON, Patrick, S. ,  MANGANIELLO, Matthew ,  CONVERTINE, Anthony ,  RATNER, Daniel, M. ,  CHOW, Yu-Hua ,  SONG, Eun-Ho ,  GHOSN, Bilal ,  SCHNAPP, Lynn ,  KELLER, Salka ,  ROY, Debashish

Inventor： STAYTON, Patrick, S. ,  MANGANIELLO, Matthew ,  CONVERTINE, Anthony ,  RATNER, Daniel, M. ,  CHOW, Yu-Hua ,  SONG, Eun-Ho ,  GHOSN, Bilal ,  SCHNAPP, Lynn ,  KELLER, Salka ,  ROY, Debashish

IPC: C08F293/00 ,  A61L27/00

CPC classification number: A61K47/48176 ,  A61K47/58 ,  C08F293/005 ,  C08F2438/03

Abstract: Provided herein are compositions and methods for intracellular delivery. The compositions are polymer compositions in which the polymer serves as a carrier for therapeutic and/or diagnostic agents. The polymer compositions are effective in targeted delivery of therapeutic and/or diagnostic agents to a cell. The polymer compositions include a targeting moiety that includes carbohydrate groups that effectively target specific cell surface receptors. The polymer compositions also include an agent binding moiety that effectively associates the therapeutic and/or diagnostic agent to be delivered to the cell.

Abstract translation: 本文提供了用于细胞内递送的组合物和方法。 组合物是其中聚合物用作治疗剂和/或诊断剂的载体的聚合物组合物。 聚合物组合物有效地向细胞靶向递送治疗剂和/或诊断剂。 聚合物组合物包括靶向部分，其包括有效靶向特定细胞表面受体的碳水化合物基团。 聚合物组合物还包括能够将待递送至细胞的治疗剂和/或诊断剂有效结合的药剂结合部分。

公开(公告)号：WO2010054266A3

公开(公告)日：2010-05-14

申请号：PCT/US2009/063648

申请日：2009-11-06

Applicant: UNIVERSITY OF WASHINGTON ,  PHASERX, INC. ,  PRIEVE, Mary, G. ,  JOHNSON, Paul, H. ,  STAYTON, Patrick, S. ,  HOFFMAN, Allan, S. ,  OVERELL, Robert, W. ,  GALL, Anna, S. ,  PASCHAL, Amber, E. E. ,  DIAB, Charbel ,  DE, Priyadarsi ,  DECLUE, Michael, S. ,  MONAHAN, Sean, D.

Inventor： PRIEVE, Mary, G. ,  JOHNSON, Paul, H. ,  STAYTON, Patrick, S. ,  HOFFMAN, Allan, S. ,  OVERELL, Robert, W. ,  GALL, Anna, S. ,  PASCHAL, Amber, E. E. ,  DIAB, Charbel ,  DE, Priyadarsi ,  DECLUE, Michael, S. ,  MONAHAN, Sean, D.

IPC: C08F297/00 ,  C08F220/18 ,  C08F220/56 ,  C08F212/06 ,  A61K48/00

Abstract: Provided herein are multiblock copolymers, as well as micelles and therapeutic compositions thereof.

公开(公告)号：WO2009140421A2

公开(公告)日：2009-11-19

申请号：PCT/US2009/043837

申请日：2009-05-13

Applicant: UNIVERSITY OF WASHINGTON ,  PHASERX, INC. ,  STAYTON, Patrick, S. ,  HOFFMAN, Allan, S. ,  CONVERTINE, Anthony, J. ,  DUVALL, Craig, L. ,  BENOIT, Danielle ,  OVERELL, Robert ,  JOHNSON, Paul ,  GALL, Anna ,  PRIEVE, Mary ,  PASCHAL, Amber ,  DIAB, Charbel ,  DE, Priyadarsi

Inventor： STAYTON, Patrick, S. ,  HOFFMAN, Allan, S. ,  CONVERTINE, Anthony, J. ,  DUVALL, Craig, L. ,  BENOIT, Danielle ,  OVERELL, Robert ,  JOHNSON, Paul ,  GALL, Anna ,  PRIEVE, Mary ,  PASCHAL, Amber ,  DIAB, Charbel ,  DE, Priyadarsi

IPC: A61K47/34 ,  A61K48/00 ,  A61K31/7088

CPC classification number: A61K47/488 ,  A61K9/1075 ,  A61K47/549 ,  A61K47/58 ,  A61K47/60 ,  A61K47/6907 ,  C08F293/005 ,  C12N15/113 ,  C12N15/87 ,  C12N2310/11 ,  C12N2310/14

Abstract: Provided herein are polymeric carriers suitable for the delivery of polynucleotides (e.g. oligonucleotides) and/or other therapeutic agents into a living cell.

Abstract translation: 本文提供适合于将多核苷酸（例如寡核苷酸）和/或其它治疗剂递送至活细胞的聚合物载体。

公开(公告)号：WO2007109584A1

公开(公告)日：2007-09-27

申请号：PCT/US2007/064238

申请日：2007-03-16

Applicant: UNIVERSITY OF WASHINGTON

Inventor： STAYTON, Patrick, S. ,  HOFFMAN, Allan, S. ,  YIN, Xiangchun ,  TAITE, Lakeshia, J. ,  GARBERN, Jessica

IPC: C08F220/54 ,  C08F220/04 ,  A61K9/70 ,  A61K47/48 ,  A61K9/51

CPC classification number: C08L53/00 ,  A61K47/58 ,  A61K47/6903 ,  A61K47/6907 ,  A61K47/6933 ,  B82Y5/00 ,  C08F8/00 ,  C08F220/04 ,  C08F220/06 ,  C08F220/54 ,  C08F220/58 ,  C08F293/00 ,  C08F293/005 ,  C08F2438/03 ,  C08F2800/20 ,  C08F2810/20 ,  C08L2312/00 ,  C08L2666/02 ,  C08L2666/04

Abstract: Temperature- and pH-responsive copolymer compositions, and drug delivery devices, conjugates, nanoparticles, and micelles that include the compositions.

Abstract translation: 温度和pH响应共聚物组合物，以及包括组合物的药物递送装置，缀合物，纳米颗粒和胶束。