公开(公告)号：US20210059241A1

公开(公告)日：2021-03-04

申请号：US16768807

申请日：2018-12-03

Applicant: University of Saskatchewan

Inventor： Francois EUDES ,  Jaswant SINGH ,  Kosala RAJAPAKSHA ,  Muhammad ANZAR ,  Gregg P. ADAMS

IPC: A01N1/02

Abstract: This disclosure provides a preserving composition comprising a cholesterol: carrier complex, optionally a cholesterol: cyclodextrin complex (CC complex) and/or a cell permeable antioxidant peptide and a biological buffer and optionally a cryprotectant, wherein the preserving composition is optionally substantially free of animal phospholipid, animal protein and/or animal lipoprotein. The disclosure also provides methods for the use of the preserving composition in cryopreservation of semen or sperm cells. Also provided is a kit comprising the preserving composition having a CC complex, a biological buffer, a cryoprotectant, a carbohydrate, a pH stabilizer, an antibiotic or antibiotic cocktail and/or a cell permeable anti-oxidant peptide.