公开(公告)号：US11819019B2

公开(公告)日：2023-11-21

申请号：US16768807

申请日：2018-12-03

Applicant: University of Saskatchewan

Inventor： Francois Eudes ,  Jaswant Singh ,  Kosala Rajapaksha ,  Muhammad Anzar ,  Gregg P. Adams

IPC: A01N1/02 ,  C12N5/076

CPC classification number: A01N1/0221 ,  A01N1/0278 ,  A01N1/0284 ,  C12N5/061

Abstract: This disclosure provides a preserving composition comprising a cholesterol: carrier complex, optionally a cholesterol:cyclodextrin complex (CC complex) and/or a cell permeable antioxidant peptide and a biological buffer and optionally a cryprotectant, wherein the preserving composition is optionally substantially free of animal phospholipid, animal protein and/or animal lipoprotein. The disclosure also provides methods for the use of the preserving composition in cryopreservation of semen or sperm cells. Also provided is a kit comprising the preserving composition having a CC complex, a biological buffer, a cryoprotectant, a carbohydrate, a pH stabilizer, an antibiotic or antibiotic cocktail and/or a cell permeable anti-oxidant peptide.

公开(公告)号：US20240237634A1

公开(公告)日：2024-07-18

申请号：US18513435

申请日：2023-11-17

Applicant: University of Saskatchewan

Inventor： Francois Eudes ,  Jaswant Singh ,  Kosala Rajapaksha ,  Muhammad Anzar ,  Gregg P. Adams

IPC: A01N1/02 ,  C12N5/076

CPC classification number: A01N1/0221 ,  A01N1/0278 ,  A01N1/0284 ,  C12N5/061

Abstract: This disclosure provides a preserving composition comprising a cholesterol:carrier complex, optionally a cholesterol:cyclodextrin complex (CC complex) and/or a cell permeable antioxidant peptide and a biological buffer and optionally a cryprotectant, wherein the preserving composition is optionally substantially free of animal phospholipid, animal protein and/or animal lipoprotein. The disclosure also provides methods for the use of the preserving composition in cryopreservation of semen or sperm cells. Also provided is a kit comprising the preserving composition having a CC complex, a biological buffer, a cryoprotectant, a carbohydrate, a pH stabilizer, an antibiotic or antibiotic cocktail and/or a cell permeable anti-oxidant peptide.