公开(公告)号：US20240254448A1

公开(公告)日：2024-08-01

申请号：US18565425

申请日：2022-05-27

Applicant: WASEDA UNIVERSITY

Inventor： Naoya TAKEDA ,  Yuji TSUCHIDO ,  Mai ONUKI

IPC: C12N5/071 ,  C12M1/12 ,  C12M3/00

CPC classification number: C12N5/0679 ,  C12M21/08 ,  C12M25/02 ,  C12N2503/04 ,  C12N2513/00 ,  C12N2533/54 ,  C12N2533/78

Abstract: The purpose of the present invention is to provide: a cell culture substrate, which is suitable for the fabrication of an in vitro biological tissue model of a biological tissue having an air-liquid interface and which enables temporal and real-time observation under an optical microscope, and a method for manufacturing the same; and a biological tissue model fabricated by using the cell culture substrate and a method for fabricating the same. A cellulose derivative membrane that is light-permeable under a wet condition by an aqueous medium is made porous to thereby impart water absorption properties suitable for a culture substrate thereto. This porous cellulose derivative membrane is combined with polymer microfibers to give a transparent dual-layer substrate as a cell culture substrate. Further, cells are cultured at an air-liquid interface using the transparent dual-layer substrate to give an in vitro biological tissue model having characteristics similar to a biological tissue.