公开(公告)号：WO1994001585A1

公开(公告)日：1994-01-20

申请号：PCT/US1993006577

申请日：1993-07-13

Applicant: WORCESTER POLYTECHNIC INSTITUTE

Inventor： WORCESTER POLYTECHNIC INSTITUTE ,  BAGSHAW, Joseph, C. ,  BUCKHOLT, Michael, A.

IPC: C12Q01/68

CPC classification number: C12Q1/6888 ,  C12Q1/6813 ,  C12Q2600/124 ,  C12Q2600/156

Abstract: A method for the selection of shrimp having a genetically-transmitted favorable growth characteristic for improved aquacultured shrimp production. A genetic marker for the favorable characteristic is prepared by isolating (Penaeus) shrimp nuclear DNA by extraction with chloroform in the presence of cetyl trimethyl ammonium bromide, digesting the DNA with one or more restriction enzymes, and identifying characteristic restriction fragments. The marker is hybridized to a nuclear nucleic acid molecule isolated from (Penaeus) shrimp for the selection of shrimp having the desired characteristic. The marker can be a labelled probe or a primer for amplification and subsequent detection of a gene that encodes a protein promoting the desired growth characteristic. The selected shrimp can be used to produce a high quality, genetically superior seedstock or larvae useful for the economic production of aquacultured shrimp.

Abstract translation: 一种用于选择具有遗传转化的有利生长特性的虾的方法，用于改进水产养殖虾生产。 通过在十六烷基三甲基溴化铵存在下用氯仿萃取分离（对虾）虾核DNA，用一种或多种限制酶消化DNA，鉴定特征性限制性片段，制备有利特征的遗传标记。 标记物与从对虾（Penaeus）虾分离的核核酸分子杂交，用于选择具有所需特征的虾。 标记可以是用于扩增和随后检测编码促进所需生长特性的蛋白质的基因的标记探针或引物。 所选择的虾可用于生产高质量，遗传上优质的种子或幼虫，用于经济生产水产养殖虾。

公开(公告)号：WO1997021835A2

公开(公告)日：1997-06-19

申请号：PCT/US1996019568

申请日：1996-12-12

Applicant: WORCESTER POLYTECHNIC INSTITUTE

Inventor： WORCESTER POLYTECHNIC INSTITUTE ,  BAGSHAW, Joseph, C. ,  BUCKHOLT, Michael, A.

IPC: C12Q01/68

CPC classification number: C12Q1/6888 ,  C12Q2600/156

Abstract: Isolated DNA from Penaeus vannamei was digested with restriction enzymes, restriction fragments of genomic DNA inserted into a plasmid vector and screened for recombinant plasmids containing repeated sequences. Ten of the resulting isolates contained representatives of the same repeated element, a satellite sequence present in one or more blocks of tandemly repeated units. The cloned repeat units range in size from about 100 to 200 base pairs, more typically between about 139 to 188 base pairs. Embedded within each cloned repeat unit are 6 to 15 copies of a tandemly repeated pentanucleotide microsatellite. The genome of P. vannamei contains approximately one million copies of this satellite/microsatellite unit. The sequences are useful as markers for the selection of shrimp having a genetically-transmitted favorable growth characteristic, such as increased reproduction, enhanced growth rate, increased size, disease-resistance, and the ability to grow in colder waters, for improved aquacultured shrimp production. Hybridization of the marker to an isolated Peaneus shrimp mucleic acid molecule can be used to identify species, strains or individual shrimp having the desired characteristics. Once identified, these shrimps can be bred to shrimp having the same or an additional desired characteristic to produce a high quality, genetically superior seedstock or larvae useful for the economic production of farmed shrimp.

Abstract translation: 用限制性酶，基因组DNA的限制性片段插入到质粒载体中消化来自对虾（Penaeus vannamei）的分离的DNA，并筛选含有重复序列的重组质粒。 所产生的分离物中的10个包含相同重复元件的代表，卫星序列存在于一个或多个串联重复单元的块中。 克隆的重复单元的大小范围为约100至200个碱基对，更典型地在约139至188个碱基对之间。 嵌入每个克隆的重复单元中是6至15个拷贝的串联重复的五核苷酸微卫星。 凡纳滨对虾的基因组包含该卫星/微型卫星单位约100万份。 这些序列可用作选择具有遗传转化的有利生长特征的虾的标记物，例如增加繁殖，增强生长速率，增加的大小，抗病性以及在较冷的水中生长的能力，以改善水产养殖虾生产 。 标记物与分离的豌豆虾核酸分子的杂交可以用于鉴定具有所需特征的物种，菌株或个体虾。 一旦鉴定出来，这些虾可以养殖到具有相同或额外的期望特性的虾，以产生用于经济生产养殖虾的高质量，遗传优良的种子或幼体。