公开(公告)号：WO1994000584A2

公开(公告)日：1994-01-06

申请号：PCT/US1993005950

申请日：1993-06-22

Applicant: WORCESTER POLYTECHNIC INSTITUTE

Inventor： WORCESTER POLYTECHNIC INSTITUTE ,  WEATHERS, Pamela, J. ,  CHEETHAM, Ronald, D. ,  DiIORIO, Alexander

IPC: C12N15/82

CPC classification number: C07D493/20 ,  C12N5/04 ,  C12N15/8205 ,  C12P17/181

Abstract: A method of transforming Artemisia annua with Agrobacterium rhizogenes. More particularly, a method for producing an Agrobacterium rhyzogenes hairy root cell culture of Artemisia annua. The method of transformation of A. annua includes the transformation of whole plants with A. rhyzogenes, and also includes transformation of A. annua root cultures with A. rhizogenes. The present invention also relates to transformed A. annua. The present invention also relates to a method for extracting enhanced amounts of a plant secondary metabolite from plant tissue with limited loss of tissue viability by reversibly permeabilizing the tissue membrane is disclosed.

Abstract translation: 用发根农杆菌转化青蒿的方法。 更具体地说，涉及一种用于生产艾蒿的根癌土壤杆菌毛根细胞培养物的方法。 A. annua的转化方法包括用A. rhyzogenes转化整株植物，还包括A.Anua根培养物与A.nizogenes的转化。 本发明还涉及转化的A. annua。 本发明还涉及一种从植物组织中提取增加量的植物次生代谢物的方法，其中通过可逆地透化组织膜来限制组织活力的丧失。