公开(公告)号：WO2021231372A1

公开(公告)日：2021-11-18

申请号：PCT/US2021/031701

申请日：2021-05-11

Applicant: WORCESTER POLYTECHNIC INSTITUTE

Inventor： REBELLO, Alex S. ,  JONES, Jordan ,  GAUDETTE, Glenn

IPC: A23L13/00 ,  C12N5/02 ,  C12N5/04 ,  C12N5/077 ,  C12M3/04 ,  A23L19/03 ,  A23L33/105 ,  A23V2002/00 ,  C12N2513/00 ,  C12N2533/90 ,  C12N5/0659

Abstract: An in vitro meat having non-decellularized leaf scaffold and a method for producing an in vitro meat using non-decellularized leaf scaffold are described herein. The method includes preparing a non-decellularized plant leaf and incubating the leaf in a cell culture media to obtain a non-decellularized leaf scaffold; seeding the leaf scaffold with a population of muscle cells to obtain a leaf scaffold adhered population of muscle cells; and growing the leaf scaffold adhered population of muscle cells in cell culture media thereby obtaining the in vitro meat.