公开(公告)号：EP1053350A1

公开(公告)日：2000-11-22

申请号：EP99903891.2

申请日：1999-02-08

Applicant: YEDA RESEARCH AND DEVELOPMENT COMPANY, LTD.

Inventor： LAPIDOT, Aviva ,  IAKOBASHVILI, Robert ,  MALIN, Gennady

IPC: C12Q1/68

CPC classification number: C12Q1/6848 ,  C12Q1/6869 ,  C12Q2527/125 ,  C12Q2531/113

Abstract: The osmoprotectants proline, 2- methyl- 4- carboxy- 3,4,5,6- tetrahydropyrimidine ('THP(B)'), and 2-methyl-4-carboxy-5-hydroxy-3,4,5,6-tetrahydropyrimidine ('THP(A)') are capable of increasing the thermal stability of DNA polymerases at elevated temperatures. THP(B) is further effective in lowering the melting temperature of double-stranded DNA. Proline, THP(A) and THP(B) are thus useful in procedures involving melting of double-stranded DNA and/or polymerase-mediated DNA synthesis, such as in primer extension, in PCR (polymerase chain reaction) amplification and in DNA sequencing.