公开(公告)号：CN106085981A

公开(公告)日：2016-11-09

申请号：CN201610507690.9

申请日：2016-06-30

Applicant: 湖南美可达生物资源有限公司

Inventor： 曾建国 ,  黄鹏 ,  卿志星

IPC: C12N9/10 ,  C12N15/54 ,  C12N15/81 ,  C12N1/19

CPC classification number: C12N9/1007 ,  C12N15/81 ,  C12N2800/102 ,  C12Y201/01116 ,  C12Y201/01117 ,  C12Y201/01122 ,  C12Y201/01128 ,  C12Y201/0114

Abstract: 本发明公开了博落回中参与血根碱与白屈菜红碱合成的甲基转移酶基因及其应用。首次在博落回基因组中发现6个参与血根碱与白屈菜红碱合成的甲基转移酶基因，包括Mc2833、Mc769、Mc8567、Mc833、Mc830、Mc9487基因。利用酿酒酵母体系验证通过上游前体饲喂分别验证了这几步反应，可实现血根碱与白屈菜红碱中间体的合成。本发明进一步揭示了血根碱在博落回中合成的分子机制，为高血根碱与白屈菜红碱含量博落回育种提供了理论依据和分子辅助育种目标；同时为血根碱与白屈菜红碱的体外人工合成提供了宝贵经验。

公开(公告)号：CN105247038A

公开(公告)日：2016-01-13

申请号：CN201480027961.X

申请日：2014-03-14

Applicant: 小利兰·斯坦福大学托管委员会

Inventor： 克里斯蒂娜·D.·斯默克 ,  凯瑟琳·托德利 ,  伊西斯·特伦查德 ,  斯蒂芬妮·格兰尼

IPC: C12N1/19 ,  C12P17/12 ,  C12P17/16 ,  C12P17/18 ,  C12P17/04 ,  C12R1/865

CPC classification number: C12P17/182 ,  C12N9/0073 ,  C12N9/1007 ,  C12N15/52 ,  C12P17/18 ,  C12Y121/03003 ,  C12Y201/01116 ,  C12Y201/01128 ,  C12Y201/0114

Abstract: 本发明的方面包括被工程化为产生苄基异喹啉生物碱(BIA)的宿主细胞。该宿主细胞包含参与宿主细胞从起始化合物到BIA的合成途径的多种酶的异源编码序列。还提供了通过在促进由宿主细胞的异源编码序列所编码的酶的表达的培养条件下培养宿主细胞来产生感兴趣的BIA的方法。本发明的方面进一步包括在本发明的方法中有用的组合物，例如，宿主细胞、起始化合物和试剂盒等。

公开(公告)号：US20160340704A1

公开(公告)日：2016-11-24

申请号：US15111041

申请日：2015-01-13

Applicant: VALORBEC SOCIÉTÉ EN COMMANDITE

Inventor： VINCENT MARTIN ,  LAUREN NARCROSS ,  ANDREW EKINS ,  ELENA FOSSATI ,  YUN ZHU

IPC: C12P17/12 ,  C12N9/10 ,  C12N15/52 ,  C07D217/04 ,  C12N15/81

CPC classification number: C12P17/12 ,  C07B2200/07 ,  C07D217/02 ,  C07D217/04 ,  C07D491/153 ,  C12N9/1007 ,  C12N15/52 ,  C12N15/81 ,  C12P17/188 ,  C12Y201/01075 ,  C12Y201/01116 ,  C12Y201/01128 ,  C12Y201/0114

Abstract: There is provided a method of preparing a benzylisoquinoline alkaloid (BIA) metabolite comprising: a. culturing a host cell under conditions suitable for protein production, including a pH of between about 7 and about 10 said host cell comprising: b. a first heterologous coding sequence encoding a first enzyme involved in a metabolite pathway that converts (R,S)-norlaudanosoline into the metabolite; c. a second heterologous coding sequence encoding a second enzyme involved in a metabolite pathway that converts (R,S)-norlaudanosoline into the metabolite; d. a third heterologous coding sequence encoding a second enzyme involved in a metabolite pathway that converts (R,S)-norlaudanosoline into the metabolite; (d) adding (R,S)-norlaudanosoline to the cell culture; and recovering the metabolite from the cell culture

Abstract translation: 提供了制备苄基异喹啉生物碱（BIA）代谢物的方法，其包括：a。 在适于蛋白质生产的条件下培养宿主细胞，包括约7至约10的pH，所述宿主细胞包含：b。 编码参与转化（R，S） - 纳多拉诺啉代谢物的代谢途径的第一酶的第一异源编码序列; C。 第二个异源编码序列，编码参与将（R，S） - 纳多拉诺啉转化成代谢物的代谢途径的第二种酶; d。 第三个异源编码序列，编码参与将（R，S） - 纳多拉诺啉转化成代谢物的代谢途径的第二种酶; （d）向细胞培养物中加入（R，S） - 纳鲁丹啉; 并从细胞培养物中回收代谢物

公开(公告)号：US20170145454A1

公开(公告)日：2017-05-25

申请号：US15360763

申请日：2016-11-23

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Christina D. Smolke ,  Catherine Thodey ,  Isis Trenchard ,  Stephanie Galanie

IPC: C12P17/18 ,  C12N9/10 ,  C12N9/02 ,  C12N15/52

CPC classification number: C12P17/182 ,  C12N9/0073 ,  C12N9/1007 ,  C12N15/52 ,  C12P17/18 ,  C12Y121/03003 ,  C12Y201/01116 ,  C12Y201/01128 ,  C12Y201/0114

Abstract: Aspects of the invention include host cells that are engineered to produce benzylisoquinoline alkaloids (BIAs). The host cells include heterologous coding sequences for a variety of enzymes involved in synthetic pathways from starting compounds to BIAs of the host cell. Also provided are methods of producing the BIAs of interest by culturing the host cells under culture conditions that promote expression of enzymes encoded by the heterologous coding sequences of the host cells. Aspects of the invention further include compositions, e.g., host cells, starting compounds and kits, etc., that find use in methods of the invention.

公开(公告)号：US09534241B2

公开(公告)日：2017-01-03

申请号：US14211611

申请日：2014-03-14

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Christina D. Smolke ,  Catherine Thodey ,  Isis Trenchard ,  Stephanie Galanie

IPC: A61K31/472 ,  C07D489/02 ,  C07D217/04 ,  C12P17/18 ,  C12N9/10 ,  C12N15/52

CPC classification number: C12P17/182 ,  C12N9/0073 ,  C12N9/1007 ,  C12N15/52 ,  C12P17/18 ,  C12Y121/03003 ,  C12Y201/01116 ,  C12Y201/01128 ,  C12Y201/0114

Abstract: Aspects of the invention include host cells that are engineered to produce benzylisoquinoline alkaloids (BIAs). The host cells include heterologous coding sequences for a variety of enzymes involved in synthetic pathways from starting compounds to BIAs of the host cell. Also provided are methods of producing the BIAs of interest by culturing the host cells under culture conditions that promote expression of enzymes encoded by the heterologous coding sequences of the host cells. Aspects of the invention further include compositions, e.g., host cells, starting compounds and kits, etc., that find use in methods of the invention.

Abstract translation: 本发明的方面包括被工程化以生产苄基异喹啉生物碱（BIAs）的宿主细胞。 宿主细胞包括参与从起始化合物到宿主细胞的BIA的合成途径的多种酶的异源编码序列。 还提供了通过在促进由宿主细胞的异源编码序列编码的酶的表达的培养条件下培养宿主细胞来生产感兴趣的BI的方法。 本发明的方面还包括可用于本发明方法的组合物，例如宿主细胞，起始化合物和试剂盒等。

公开(公告)号：EP2970999A2

公开(公告)日：2016-01-20

申请号：EP14729501.8

申请日：2014-03-14

Applicant: The Board Of Trustees Of The University Of the Leland Stanford Junior University

Inventor： SMOLKE, Christina D. ,  THODEY, Catherine ,  TRENCHARD, Isis ,  GALANIE, Stephanie

IPC: C12N15/82

CPC classification number: C12P17/182 ,  C12N9/0073 ,  C12N9/1007 ,  C12N15/52 ,  C12P17/18 ,  C12Y121/03003 ,  C12Y201/01116 ,  C12Y201/01128 ,  C12Y201/0114

Abstract: Aspects of the invention include host cells that are engineered to produce benzylisoquinoline alkaloids (BIAs). The host cells include heterologous coding sequences for a variety of enzymes involved in synthetic pathways from starting compounds to BIAs of the host cell. Also provided are methods of producing the BIAs of interest by culturing the host cells under culture conditions that promote expression of enzymes encoded by the heterologous coding sequences of the host cells. Aspects of the invention further include compositions, e.g., host cells, starting compounds and kits, etc., that find use in methods of the invention.

Abstract translation: 本发明的方面包括宿主细胞工程化以产生没有苄基异生物碱（偏压）。 该宿主细胞包括用于各种从起始化合物与宿主细胞的偏压在合成途径所涉及的酶的异源编码序列。 所以提供了通过培养的培养条件下的宿主细胞产生感兴趣的偏压的方法都促进由宿主细胞的异源编码序列编码的酶的表达。 本发明的方面进一步包括组合物，E. G.，宿主细胞，起始化合物和试剂盒等，确实发现在发明的方法中使用。

公开(公告)号：US20180334695A1

公开(公告)日：2018-11-22

申请号：US15978005

申请日：2018-05-11

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Christina D. Smolke ,  Catherine Thodey ,  Isis Trenchard ,  Stephanie Galanie

IPC: C12P17/18 ,  C12N9/02 ,  C12N15/52 ,  C12N9/10

CPC classification number: C12P17/182 ,  C12N9/0073 ,  C12N9/1007 ,  C12N15/52 ,  C12P17/18 ,  C12Y121/03003 ,  C12Y201/01116 ,  C12Y201/01128 ,  C12Y201/0114

Abstract: Aspects of the invention include host cells that are engineered to produce benzylisoquinoline alkaloids (BIAs). The host cells include heterologous coding sequences for a variety of enzymes involved in synthetic pathways from starting compounds to BIAs of the host cell. Also provided are methods of producing the BIAs of interest by culturing the host cells under culture conditions that promote expression of enzymes encoded by the heterologous coding sequences of the host cells. Aspects of the invention further include compositions, e.g., host cells, starting compounds and kits, etc., that find use in methods of the invention.

公开(公告)号：US20170130250A1

公开(公告)日：2017-05-11

申请号：US15319568

申请日：2015-06-12

Applicant: EPIMERON INC.

Inventor： Peter James Facchini

IPC: C12P17/12

CPC classification number: C12P17/12 ,  C12N9/0022 ,  C12N9/0059 ,  C12N9/88 ,  C12N15/8243 ,  C12P7/24 ,  C12P13/001 ,  C12P13/22 ,  C12P17/182 ,  C12Y201/01128

Abstract: Methods that may be used for the manufacture of the chemical compound (S)-norcoclaurine, (S)-norlaudanosoline, and (S)-norcoclaurine or [S]-norlaudanosoline synthesis intermediates are provided. (S)-Norcoclaurine, (S)-norlaudanosoline, and (S)-norcoclaurine or (S)-norlaudanosoline synthesis intermediates are useful as precursor products in the manufacture of certain medicinal agents.

公开(公告)号：US20140273109A1

公开(公告)日：2014-09-18

申请号：US14211611

申请日：2014-03-14

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Christina D. Smolke ,  Catherine Thodey ,  Isis Trenchard ,  Stephanie Galanie

IPC: C12P17/18 ,  C12N15/81

CPC classification number: C12P17/182 ,  C12N9/0073 ,  C12N9/1007 ,  C12N15/52 ,  C12P17/18 ,  C12Y121/03003 ,  C12Y201/01116 ,  C12Y201/01128 ,  C12Y201/0114

Abstract: Aspects of the invention include host cells that are engineered to produce benzylisoquinoline alkaloids (BIAs). The host cells include heterologous coding sequences for a variety of enzymes involved in synthetic pathways from starting compounds to BIAs of the host cell. Also provided are methods of producing the BIAs of interest by culturing the host cells under culture conditions that promote expression of enzymes encoded by the heterologous coding sequences of the host cells. Aspects of the invention further include compositions, e.g., host cells, starting compounds and kits, etc., that find use in methods of the invention.

Abstract translation: 本发明的方面包括被工程化以生产苄基异喹啉生物碱（BIAs）的宿主细胞。 宿主细胞包括参与从起始化合物到宿主细胞的BIA的合成途径的多种酶的异源编码序列。 还提供了通过在促进由宿主细胞的异源编码序列编码的酶的表达的培养条件下培养宿主细胞来生产感兴趣的BI的方法。 本发明的方面还包括可用于本发明方法的组合物，例如宿主细胞，起始化合物和试剂盒等。

公开(公告)号：WO2014143744A2

公开(公告)日：2014-09-18

申请号：PCT/US2014027833

申请日：2014-03-14

Applicant: UNIV LELAND STANFORD JUNIOR

Inventor： SMOLKE CHRISTINA D ,  THODEY CATHERINE ,  TRENCHARD ISIS ,  GALANIE STEPHANIE

IPC: C12N1/00

CPC classification number: C12P17/182 ,  C12N9/0073 ,  C12N9/1007 ,  C12N15/52 ,  C12P17/18 ,  C12Y121/03003 ,  C12Y201/01116 ,  C12Y201/01128 ,  C12Y201/0114

Abstract: Aspects of the invention include host cells that are engineered to produce benzylisoquinoline alkaloids (BIAs). The host cells include heterologous coding sequences for a variety of enzymes involved in synthetic pathways from starting compounds to BIAs of the host cell. Also provided are methods of producing the BIAs of interest by culturing the host cells under culture conditions that promote expression of enzymes encoded by the heterologous coding sequences of the host cells. Aspects of the invention further include compositions, e.g., host cells, starting compounds and kits, etc., that find use in methods of the invention.

Abstract translation: 本发明的方面包括被工程化以生产苄基异喹啉生物碱（BIAs）的宿主细胞。 宿主细胞包括参与从起始化合物到宿主细胞的BIA的合成途径的多种酶的异源编码序列。 还提供了通过在促进由宿主细胞的异源编码序列编码的酶的表达的培养条件下培养宿主细胞来生产感兴趣的BI的方法。 本发明的方面还包括可用于本发明方法的组合物，例如宿主细胞，起始化合物和试剂盒等。