公开(公告)号：EP1192446A4

公开(公告)日：2002-08-21

申请号：EP00928251

申请日：2000-04-20

Applicant: CHROMAGEN

Inventor： GOULD GENE R ,  CONRAD MICHAEL

IPC: G01J3/443 ,  G01J3/02 ,  G01J3/06 ,  G01J3/12 ,  G01J3/18 ,  G01J3/44 ,  G01N21/03 ,  G01N21/25 ,  G01N21/64

CPC classification number: G01J3/06 ,  G01J1/0437 ,  G01J3/02 ,  G01J3/12 ,  G01J3/18 ,  G01J3/1838 ,  G01J3/4406 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6417 ,  G01N2021/6463 ,  G01N2021/6482

Abstract: A fluorescence spectrophotometer having an excitation double monochromator (104), a coaxial excitation/emission light transfer module (106), and an emission double monochromator (110). Each monochromator includes a pair of holographic concave gratings (203, 210) mounted to precisely select a desired band of wavelengths from incoming broadband light without using other optical elements, such as mirrors. Selected excitation light is directed into a sample well (108) by a light transfer module (106) that includes a coaxial excitation mirror (302) positioned to directed excitation light directly to the bottom of a well (108) of a multi-well plate. Fluorescence emission light that exits the well opening is collected by a relatively large coaxial emission mirror (304). The collected emission light is wavelength selected by the emission double monochromator (110). Selected emission light is detected by a photodetector module (112).