公开(公告)号：US09925220B2

公开(公告)日：2018-03-27

申请号：US14674548

申请日：2015-03-31

Applicant: University Health Network

Inventor： Li Zhang ,  Mei Han ,  Pouneh Dokouhaki

IPC: C12N5/0783 ,  A61K35/17

CPC classification number: A61K35/17 ,  C12N5/0636 ,  C12N2501/23 ,  C12N2501/2302 ,  C12N2501/2304 ,  C12N2501/515

Abstract: A method of expanding double negative T cells in culture is described. The method comprises (a) providing a starting sample comprising DN T cells or precursors thereof; (b) substantially depleting CD8+ and CD4+ T cells from the starting sample; (c) culturing the sample from step (b) with an immobilized T cell mitogen in a culture medium comprising an agent that can stimulate DN T cell growth (d) washing the cells obtained in step (c) and resuspending in a culture medium comprising the agent without the T cell mitogen; and (e) washing the cells obtained in step (d) and resuspending in a culture medium comprising the agent and a soluble T cell mitogen. The DN T cells obtained by the method are useful in a variety of applications including the treatment of cancer, infectious diseases, graft versus host disease and autoimmune disease.

公开(公告)号：US20160045550A1

公开(公告)日：2016-02-18

申请号：US14674548

申请日：2015-03-31

Applicant: University Health Network

Inventor： Li Zhang ,  Mei Han ,  Pouneh Dokouhaki

IPC: A61K35/17 ,  C12N5/0783

CPC classification number: A61K35/17 ,  C12N5/0636 ,  C12N2501/23 ,  C12N2501/2302 ,  C12N2501/2304 ,  C12N2501/515

Abstract: A method of expanding double negative T cells in culture is described. The method comprises (a) providing a starting sample comprising DN T cells or precursors thereof; (b) substantially depleting CD8+ and CD4+ T cells from the starting sample; (c) culturing the sample from step (b) with an immobilized T cell mitogen in a culture medium comprising an agent that can stimulate DN T cell growth (d) washing the cells obtained in step (c) and resuspending in a culture medium comprising the agent without the T cell mitogen; and (e) washing the cells obtained in step (d) and resuspending in a culture medium comprising the agent and a soluble T cell mitogen. The DN T cells obtained by the method are useful in a variety of applications including the treatment of cancer, infectious diseases, graft versus host disease and autoimmune disease.

Abstract translation: 描述培养培养中双阴性T细胞的方法。 该方法包括（a）提供包含DN T细胞或其前体的起始样品; （b）从起始样品中大量消耗CD8 +和CD4 + T细胞; （c）在包含可以刺激DN T细胞生长的试剂的培养基中培养来自步骤（b）的来自步骤（b）的样品的固定化的T细胞丝裂原，（d）洗涤步骤（c）中获得的细胞并重新悬浮于培养基中，所述培养基包含 没有T细胞有丝分裂原的药剂; 和（e）洗涤步骤（d）中获得的细胞，并重新悬浮于包含该试剂和可溶性T细胞丝裂原的培养基中。 通过该方法获得的DN T细胞可用于各种应用，包括治疗癌症，感染性疾病，移植物抗宿主病和自身免疫性疾病。