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    Method of expanding double negative T cells
    2.
    发明授权
    Method of expanding double negative T cells 有权

    公开(公告)号：US09925220B2

    公开(公告)日：2018-03-27

    申请号：US14674548

    申请日：2015-03-31

    Applicant: University Health Network

    Inventor： Li Zhang ,  Mei Han ,  Pouneh Dokouhaki

    IPC: C12N5/0783 ,  A61K35/17

    CPC classification number: A61K35/17 ,  C12N5/0636 ,  C12N2501/23 ,  C12N2501/2302 ,  C12N2501/2304 ,  C12N2501/515

    Abstract: A method of expanding double negative T cells in culture is described. The method comprises (a) providing a starting sample comprising DN T cells or precursors thereof; (b) substantially depleting CD8+ and CD4+ T cells from the starting sample; (c) culturing the sample from step (b) with an immobilized T cell mitogen in a culture medium comprising an agent that can stimulate DN T cell growth (d) washing the cells obtained in step (c) and resuspending in a culture medium comprising the agent without the T cell mitogen; and (e) washing the cells obtained in step (d) and resuspending in a culture medium comprising the agent and a soluble T cell mitogen. The DN T cells obtained by the method are useful in a variety of applications including the treatment of cancer, infectious diseases, graft versus host disease and autoimmune disease.

    Immunotherapy for the treatment of cancer
    4.
    发明授权
    Immunotherapy for the treatment of cancer 有权

    公开(公告)号：US10517937B2

    公开(公告)日：2019-12-31

    申请号：US15503916

    申请日：2015-08-17

    Applicant: University Health Network

    Inventor： Li Zhang ,  Jong Bok Lee ,  Branson Chen

    IPC: A61K35/17 ,  A61K39/00 ,  A61K31/7068

    Abstract: Methods for the treatment of cancer using double negative (DN) T cells are described. The DNTs may be used for the treatment of chemotherapy-resistant cancers such as recurring or relapsing acute myeloid leukemia (AML). The use of allogenic DNTs, such as those derived from healthy donors, that do not exhibit toxicity towards normal host tissues and the complications associated with graft-versus-host-disease, is also described.

    Method of Expanding Double Negative T Cells
    8.
    发明申请
    Method of Expanding Double Negative T Cells 有权
    扩大双重阴性T细胞的方法

    公开(公告)号：US20160045550A1

    公开(公告)日：2016-02-18

    申请号：US14674548

    申请日：2015-03-31

    Applicant: University Health Network

    Inventor： Li Zhang ,  Mei Han ,  Pouneh Dokouhaki

    IPC: A61K35/17 ,  C12N5/0783

    CPC classification number: A61K35/17 ,  C12N5/0636 ,  C12N2501/23 ,  C12N2501/2302 ,  C12N2501/2304 ,  C12N2501/515

    Abstract: A method of expanding double negative T cells in culture is described. The method comprises (a) providing a starting sample comprising DN T cells or precursors thereof; (b) substantially depleting CD8+ and CD4+ T cells from the starting sample; (c) culturing the sample from step (b) with an immobilized T cell mitogen in a culture medium comprising an agent that can stimulate DN T cell growth (d) washing the cells obtained in step (c) and resuspending in a culture medium comprising the agent without the T cell mitogen; and (e) washing the cells obtained in step (d) and resuspending in a culture medium comprising the agent and a soluble T cell mitogen. The DN T cells obtained by the method are useful in a variety of applications including the treatment of cancer, infectious diseases, graft versus host disease and autoimmune disease.

    Abstract translation: 描述培养培养中双阴性T细胞的方法。 该方法包括（a）提供包含DN T细胞或其前体的起始样品; （b）从起始样品中大量消耗CD8 +和CD4 + T细胞; （c）在包含可以刺激DN T细胞生长的试剂的培养基中培养来自步骤（b）的来自步骤（b）的样品的固定化的T细胞丝裂原，（d）洗涤步骤（c）中获得的细胞并重新悬浮于培养基中，所述培养基包含 没有T细胞有丝分裂原的药剂; 和（e）洗涤步骤（d）中获得的细胞，并重新悬浮于包含该试剂和可溶性T细胞丝裂原的培养基中。 通过该方法获得的DN T细胞可用于各种应用，包括治疗癌症，感染性疾病，移植物抗宿主病和自身免疫性疾病。

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