公开(公告)号：US11573118B2

公开(公告)日：2023-02-07

申请号：US16892502

申请日：2020-06-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Glen Davis

IPC: G01N29/22 ,  G01F25/17 ,  G01F15/00

Abstract: Provided are systems and methods for line volume calibration, and measurement of fluid samples delivered to an interrogation point. In various embodiments, a known fluid volume comprising a sample line fluid and a secondary fluid is delivered to a fluid boundary sensor. The fluid boundary sensor assists in determining the position of the boundaries between the various fluids, and the positions of these boundaries are used to determine the sample line fluid volume.

公开(公告)号：US20230022224A1

公开(公告)日：2023-01-26

申请号：US17861854

申请日：2022-07-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Harrison LEONG ,  Nivedita Sumi MAJUMDAR ,  Jeffrey A. MARKS ,  Theodore E. STRAUB ,  Ryan J. TALBOT ,  Kevin S. BODNER ,  David HOARD

IPC: G06T11/20 ,  G16B45/00

Abstract: A method comprises displaying, on a graphical user interface (GUI), a first data visualization comprising a positional representation of a plurality of reaction sites from at least a portion of a substrate, wherein the positional representation of the first data visualization simultaneously indicates relative data quality of the plurality of reaction sites; and altering the first data visualization displayed on the GUI to a second data visualization, the second data visualization comprising the positional representation of the plurality of reaction sites from the at least the portion of the substrate, wherein the positional representation of the second data visualization simultaneously indicates relative data quality of the plurality of reaction sites from at least the portion of the substrate based on a comparison of the data quality values of the plurality of reaction sites an adjusted quality value threshold.

公开(公告)号：USD975873S1

公开(公告)日：2023-01-17

申请号：US29741481

申请日：2020-07-13

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES HOLDINGS PTE LIMITED

Designer： Marie Murakami ,  Kyle Bulloch ,  Neil Olson ,  Joshua Mead ,  Ross Winnick ,  Wei Fuh Teo ,  Michael Thacker

公开(公告)号：US11542540B2

公开(公告)日：2023-01-03

申请号：US16622113

申请日：2018-06-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Dalia Dhingra ,  Richard Chien

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Provided are methods, as well as compositions, kits, and systems for preparing optimized control nucleic acids (polynucleotides) having reduce nucleic acid damage. Provided nucleic acid compositions provide reduced artifacts as compared to nucleic acid compositions prepared by conventional methods. Provided compositions are useful control in a variety of applications, including, but not limited to sequencing workflows to effectively monitor sensitivity, accuracy and/or precision of data.

公开(公告)号：US20220412890A1

公开(公告)日：2022-12-29

申请号：US17825222

申请日：2022-05-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kevin MAHER ,  Mingsong CHEN ,  Jacob K. FREUDENTHAL ,  Kuan Moon BOO ,  Soo Yong LAU ,  David FORTESCUE ,  Ming SHEN ,  Woon Liang Terence SOH

IPC: G01N21/64

Abstract: An instrument for processing and/or measuring a biological process comprises a plurality of filter assemblies configured to be interchangeably located along at least one of the optical paths. The plurality of filter components includes a first filter assembly characterized by a first optical power and a first filter having a first filter function, the first filter function characterized by at least one of a first low-pass wavelength or a first high-pass wavelength. The second filter assembly is characterized by a second optical power and a second filter having a second filter function, the second filter function comprising at least one of a second low-pass wavelength that is different than the first low-pass wavelength or a second high-pass wavelength that is different than the first high-pass wavelength.

公开(公告)号：US11513133B2

公开(公告)日：2022-11-29

申请号：US16194187

申请日：2018-11-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kristopher Barbee ,  Maximilian Carpino ,  Ray Wheeling ,  Nicholas Bajka

IPC: G01N35/00 ,  G01N35/10

Abstract: The present teachings provide apparatuses and methods for automated handling of samples, e.g., biological or chemical samples. The apparatuses and the methods of the present teachings allow automated performance of various sample manipulation steps without manual intervention. In a preferred embodiment, the present teachings provide apparatuses and methods for automated enrichment of templated beads produced by PCR.

公开(公告)号：US20220372435A1

公开(公告)日：2022-11-24

申请号：US17761879

申请日：2020-09-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mwita PHELPS

IPC: C12N5/00

Abstract: Described herein are efficiently dissolving tablets of dry cell culture media, feeds, supplements, media subgroups, buffer concentrates, or media components useful in culturing cells or microorganisms, methods of manufacturing, and methods of use. In particular, formulations of tableted cell culture media feeds, supplements, media subgroups, or buffer concentrates are described.

公开(公告)号：US11505827B2

公开(公告)日：2022-11-22

申请号：US16351456

申请日：2019-03-12

Applicant: Roche Sequencing Solutions, Inc. ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Timothy Geiser ,  Randall Davis ,  Roger Chen

IPC: C12M1/34 ,  C12Q1/6874 ,  C12Q1/6869 ,  B82Y15/00

Abstract: Systems and methods of polynucleotide sequencing are provided. Systems and methods optimize control, speed, movement, and/or translocation of a sample (e.g., a polynucleotide) within, through, or at least partially through a nanopore or a type of protein or mutant protein in order to accumulate sufficient time and current blocking information to identify contiguous nucleotides or plurality of nucleotides in a single-stranded area of a polynucleotide.

公开(公告)号：US20220349807A1

公开(公告)日：2022-11-03

申请号：US17729440

申请日：2022-04-26

Applicant: Life Technologies Corporation

Inventor： Eric Finan ,  Michael Ward

IPC: G01N15/14

Abstract: Provided are, inter alia, flow centration components that can be used in flow cytometers and other applications. A flow centrator can define central axis, a proximal end, and a distal end, and having a central bore extending within the flow centration component in the direction of the central axis; the flow centration component defining a splined outer surface that defines a plurality of circumferentially arranged bypass flute channels, the plurality of bypass flute channels extending in the direction of the central axis, and each of the bypass flute channels having a depth and a length. Also provided are related methods that utilize the disclosed components.

公开(公告)号：US20220348964A1

公开(公告)日：2022-11-03

申请号：US17860833

申请日：2022-07-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Xin YU ,  Xavier de MOLLERAT du JEU

IPC: C12N15/86 ,  C12N7/00

Abstract: A lentiviral vector production system comprises (a) a lentiviral culture supplement to control cell growth, (b) a transfection reagent comprising DHDMS, DOPE, and cholesterol to increase transfection efficiency, (c) a lentiviral production enhancer comprising sodium propionate, sodium butyrate, and caffeine to boost lentiviral production, wherein the lentiviral vector production system is serum-free. A method of lentiviral vector production comprises using the lentiviral production system. Another method for lentiviral vector production comprises (a) culturing eukaryotic cells in a serum-free medium, (b) providing a lentiviral culture supplement to control cell growth, (c) transfecting the cells with a lentiviral vector using a transfection reagent comprising DHDMS, DOPE, and cholesterol to increase transfection efficiency, and (d) providing a lentiviral production using a lentiviral production enhancer comprising sodium propionate, sodium butyrate capable of boosting lentiviral production.