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    Droplet-based pyrosequencing
    116.
    发明授权
    Droplet-based pyrosequencing 有权
    基于液滴的焦磷酸测序

    公开(公告)号：US09243282B2

    公开(公告)日：2016-01-26

    申请号：US13470919

    申请日：2012-05-14

    Applicant: Michael G. Pollack ,  Vamsee K. Pamula ,  Richard B. Fair ,  Peter B. Griffin

    Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Richard B. Fair ,  Peter B. Griffin

    IPC: C12Q1/68 ,  C12P19/34 ,  G01N21/00 ,  G01N27/447

    CPC classification number: C12Q1/68 ,  B01L7/52 ,  B01L2300/0645 ,  B01L2300/1822 ,  B01L2300/1894 ,  B01L2400/0424 ,  B01L2400/0427 ,  C12Q1/6869 ,  G01N27/44791 ,  Y10T436/25 ,  C12Q2565/629 ,  C12Q2565/301 ,  C12Q2563/149

    Abstract: The present invention relates to droplet-based pyrosequencing including a method of identifying a base at a target position in a sample nucleic acid. The method includes: (a) providing a droplet microactuator including a first droplet including a sample nucleic acid immobilized on a bead; and (b) on the droplet microactuator: (i) contacting the first droplet with one or more reagent droplets to yield a second droplet, wherein the one or more reagent droplets include reagents for extending a double stranded portion of the sample nucleic acid by incorporating a nucleotide at the target position; (ii) splitting the second droplet to yield a third droplet including the bead and a fourth droplet lacking the bead; and (iii) assaying the third droplet to determine whether the nucleotide was incorporated at the target position.

    Abstract translation: 本发明涉及基于液滴的焦磷酸测序，包括鉴定样品核酸中目标位置的碱基的方法。 该方法包括：（a）提供包括包含固定在珠粒上的样品核酸的第一液滴的液滴微致动器; 和（b）在所述液滴微致动器上：（i）使所述第一液滴与一个或多个试剂液滴接触以产生第二液滴，其中所述一个或多个试剂液滴包括用于通过掺入来扩展样品核酸的双链部分的试剂 目标位置的核苷酸; （ii）分裂第二液滴以产生包含珠粒的第三液滴和不含珠粒的第四液滴; 和（iii）测定第三液滴以确定核苷酸是否并入目标位置。

    Microfluidic feedback using impedance detection
    117.
    发明授权
    Microfluidic feedback using impedance detection 有权
    微流体反馈采用阻抗检测

    公开(公告)号：US09188615B2

    公开(公告)日：2015-11-17

    申请号：US14116553

    申请日：2012-05-08

    Applicant: Ryan Sturmer ,  Vijay Srinivasan ,  Arjun Sudarsan

    Inventor： Ryan Sturmer ,  Vijay Srinivasan ,  Arjun Sudarsan

    IPC: G01R27/16 ,  B01L3/00

    CPC classification number: B01L3/50273 ,  B01L3/502792 ,  B01L2200/0621 ,  B01L2200/10 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/087 ,  B01L2300/089 ,  B01L2400/0427 ,  G01N27/048 ,  G01R27/16

    Abstract: Methods comprising measuring the impedance of the electrode produced by the excitation signal, wherein the impedance indicates presence of liquid at the electrode are disclosed. Computer readable mediums storing processor executable instructions for performing the method, and systems are also disclosed. The systems comprise a processor, memory and code stored in the memory that when executed cause the processor at least to: receive an output voltage signal, superimpose an excitation signal onto the output voltage signal to produce a superimposed signal, connect the superimposed signal to an electrode in a droplet actuator, suppress the output voltage signal, when detecting an impedance of the electrode, and measure the impedance of the electrode produced by the excitation signal, wherein the impedance indicates presence of liquid at the electrode.

    Abstract translation: 方法包括测量由激发信号产生的电极的阻抗，其中阻抗表示在电极处存在液体。 还公开了存储用于执行该方法的处理器可执行指令的计算机可读介质，以及系统。 系统包括存储在存储器中的处理器，存储器和代码，当执行时，处理器至少要使接收输出电压信号，将激励信号叠加在输出电压信号上，产生叠加信号，将叠加的信号连接到 液滴致动器中的电极，当检测到电极的阻抗时抑制输出电压信号，并测量由激励信号产生的电极的阻抗，其中阻抗表示在电极处存在液体。

    ACYL-COA DEHYDROGENASE ASSAYS
    119.
    发明申请
    ACYL-COA DEHYDROGENASE ASSAYS 审中-公开
    ACYL-COA脱氢酶测定

    公开(公告)号：US20150267242A1

    公开(公告)日：2015-09-24

    申请号：US14439255

    申请日：2013-10-30

    Applicant: ADVANCED LIQUID LOGIC, INC

    Inventor： Nora J. Foegeding ,  Carrie A. Graham ,  Allen E. Eckhardt

    IPC: C12Q1/32

    CPC classification number: C12Q1/32 ,  G01N33/6893 ,  G01N2333/90206 ,  G01N2800/04 ,  G01N2800/7085

    Abstract: A method for conducting medium-chain acyl-CoA dehydrogenase (MCAD) and very-long-chain acyl-CoA dehydrogenase (VCAD) enzymatic activity assays is provided. The method may include, but is not limited to, preparing a sample; preparing an enzyme-specific substrate/reagent mixture; mixing an aliquot of the prepared sample with an aliquot of the enzyme-specific substrate/reagent mixture; reading absorbance in the range of about 600 nm; incubating the prepared sample and enzyme-specific substrate/reagent mixture; and reading absorbance in the range of about 600 nm at various time intervals.

    Abstract translation: 提供了一种用于进行中链酰基辅酶A脱氢酶（MCAD）和非常长链的酰基辅酶A脱氢酶（VCAD）酶活性测定的方法。 该方法可以包括但不限于制备样品; 制备酶特异性底物/试剂混合物; 将制备的样品的等分试样与酶特异性底物/试剂混合物的等分试样混合; 读取吸光度在约600nm范围内; 孵育制备的样品和酶特异性底物/试剂混合物; 并以各种时间间隔读取约600nm范围内的吸光度。

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