公开(公告)号：US11101019B2

公开(公告)日：2021-08-24

申请号：US15834520

申请日：2017-12-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ruchi Chaudhary ,  Fiona Hyland

IPC: G16B20/00 ,  G16B30/00

Abstract: A targeted panel with low sample input requirements from a tumor only sample may be processed to estimate mutation load in a tumor sample. The method may include: detecting variants in nucleic acid sequence reads corresponding to targeted locations in the tumor sample genome; annotating detected variants with an annotation information from a population database; filtering the detected variants, wherein the filtering rule set retains the somatic variants and removes germ-line variants; counting the identified somatic variants to give a number of somatic variants; determining a number of bases in covered regions of the targeted locations in the tumor sample genome; and calculating a number of somatic variants per megabase, provides an estimate of the mutation load per megabase in the tumor sample genome.

公开(公告)号：US11091801B2

公开(公告)日：2021-08-17

申请号：US15198689

申请日：2016-06-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Christopher Trinh ,  Tom Xu ,  Yating Shi ,  Ferrier Le ,  Claire Marjoribanks

IPC: C12Q1/686 ,  C12Q1/6848

Abstract: A composition comprising a thermostable DNA polymerase; and a PCR inhibitor blocking agent, wherein the PCR inhibitor blocking agent is present in an amount effective to enhance tolerance of an assembled PCR to a PCR inhibitor.

公开(公告)号：US11072824B2

公开(公告)日：2021-07-27

申请号：US16656839

申请日：2019-10-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shiaw-Min Chen ,  Elana Swartzman ,  David Ruff ,  Mark Shannon ,  Julia Lu ,  Stephen Hendricks

IPC: C12Q1/68 ,  C12Q1/6855 ,  C12Q1/6804

Abstract: This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.

公开(公告)号：US20210170389A1

公开(公告)日：2021-06-10

申请号：US16970444

申请日：2019-02-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Andrew TOMLINSON ,  Matthew BUCHANAN ,  Kelly FLOOK ,  Kevin GALIPEAU

IPC: B01J39/05 ,  B01J39/20 ,  B01J47/02 ,  B01J47/014 ,  C07K1/18 ,  B01D15/36

Abstract: Provided herein are compositions, methods and uses that relate to or result from providing separation media having at least one flocculant ligand covalently attached to a base surface or support, and the separation and/or purification of biological molecules using the separation media of the present disclosure. Certain embodiments provide separation media which under certain modes of operation, enhance the separation of the molecule of interest from impurities.

公开(公告)号：US11028438B2

公开(公告)日：2021-06-08

申请号：US16866653

申请日：2020-05-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Todd Rearick ,  Mark Milgrew ,  Jonathan Schultz ,  Chris Papalias ,  Kim Johnson

IPC: C12Q1/6874 ,  G16B30/00

Abstract: In one implementation, a method is described. The method includes determining an operational characteristic of sensors of a sensor array. The method further includes selecting a group of sensors in the array based on the operational characteristic of sensors in the group. The method further includes enabling readout of the sensors in the selected group. The method further includes receiving output signals from the enabled sensors.

公开(公告)号：US20210164047A1

公开(公告)日：2021-06-03

申请号：US17248722

申请日：2021-02-04

Applicant: Life Technologies Corporation

Inventor： Timothy LOONEY ,  Geoffrey LOWMAN ,  Lifeng LIN

IPC: C12Q1/6876 ,  C12Q1/6869 ,  C12Q1/6844 ,  C12Q1/6811 ,  C12Q1/6881 ,  C12Q1/6883 ,  C40B50/06

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US20210155466A1

公开(公告)日：2021-05-27

申请号：US17165196

申请日：2021-02-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Todd ROSWECH ,  Jonathan SCHULTZ ,  Chun HO

IPC: B67D7/02 ,  B67D7/76 ,  B01L3/00

Abstract: A fluidic interconnect includes a first interface including a liquid port, a gas port, and a cradle; a second interface including a liquid port, a gas port, and a swing bar to engage the cradle, a weight of a container attached to one of the first or second interfaces to drive the liquid port of the first interface into connection with the liquid port of the second interface and the gas port of the first interface into connection with the gas port of the second interface.

公开(公告)号：US20210154683A1

公开(公告)日：2021-05-27

申请号：US17169030

申请日：2021-02-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kristopher BARBEE ,  Rui ZHENG ,  Sean MCCUSKER

IPC: B04B7/08 ,  B04B5/04 ,  B01D21/26

Abstract: A rotor assembly includes a rotor plate to rotate around a first axis; a bucket rotatably attached to the rotor plate and to rotate around a second axis; and a stop plate to rotate around the first axis relative to the rotor plate between an open position and a closed position, when in the closed position, the stop plate is to engage the bucket to fix an angular position of the bucket relative to a plane of rotation of the rotor assembly.

公开(公告)号：US20210130913A1

公开(公告)日：2021-05-06

申请号：US17099621

申请日：2020-11-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： CHANG ZHONG

IPC: C12Q1/6888 ,  C12Q1/6881 ,  C12Q1/6858

Abstract: Methods for human identification using polymorphisms in the Penta E short tandem repeat locus are significant in preventing allelic drop out. An exemplary method encompasses (a) contacting a first primer to a nucleic acid sample to be analyzed, (b) contacting a second primer to the nucleic acid sample, and (c) subjecting the nucleic acid sample, the first primer, and the second primer to an amplification reaction, and thereby forming an amplification product. The first primer, the second primer, or both the first and second primers can be labeled with a non-nucleic-acid label. Additionally, or alternatively, the amplification product can include an adenosine at position 14 from the 5′ end of SEQ ID NO:1, a thymidine at position 21 from the 5′ end of SEQ ID NO:2, or both an adenine at position 14 and a thymidine at position 21 from the 5′ end of SEQ ID NO:3.

公开(公告)号：US20210116414A1

公开(公告)日：2021-04-22

申请号：US17063621

申请日：2020-10-05

Applicant: Life Technologies Corporation

Inventor： Todd REARICK ,  Melville DAVEY ,  Mark BEAUCHEMIN

IPC: G01N27/416 ,  G16B30/00 ,  G01N27/414

Abstract: A method, computer program product, and system are provided to calibrate a sensor array with a plurality of sensors. The method can include sweeping a voltage of a reference electrode from a first voltage to a second voltage, where the reference electrode is in fluid communication with the sensor array. The output voltage of each of the plurality of sensors can be monitored at one or more voltages within the first and second voltages. An overall average gain of the plurality of sensors can be calculated at each of the one or more voltages. Further, an acquisition window for the sensor array can be determined. The acquisition window can include a maximum distribution of sensors that provides a maximal overall average gain at a particular reference electrode voltage.