公开(公告)号：US07301628B2

公开(公告)日：2007-11-27

申请号：US11404430

申请日：2006-04-13

Applicant: Brian T. Cunningham ,  Peter Y. Li ,  Frank DiFilippi

Inventor： Brian T. Cunningham ,  Peter Y. Li ,  Frank DiFilippi

IPC: G01J3/28

CPC classification number: G01N21/253 ,  B01L3/5085 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/0829 ,  G01N21/4788 ,  G01N21/7743 ,  G01N33/54373 ,  G01N2201/0635 ,  G01N2201/08 ,  G01N2201/0833 ,  G01N2201/0846 ,  G01N2201/1248 ,  G02B5/1809 ,  G02B5/1814 ,  G02B21/06

Abstract: Method and apparatus for detecting biomolecular interactions. The use of labels is not required and the methods may be performed in a high-throughput manner. An apparatus for detecting biochemical interactions occurring on the surface of a biosensor includes a light source. A first optical fiber is coupled to the light source and illuminates the biosensor. A second optical fiber detects a wavelength reflected from the biosensor. A spectrometer determines spectra of a reflected signal from the biosensor.

公开(公告)号：US07211390B2

公开(公告)日：2007-05-01

申请号：US10788529

申请日：2004-02-26

Applicant: Jonathan M. Rothberg ,  Joel S. Bader ,  Scott B. Dewell ,  Keith McDade ,  John W. Simpson ,  Jan Berka ,  Christopher M. Colangelo

Inventor： Jonathan M. Rothberg ,  Joel S. Bader ,  Scott B. Dewell ,  Keith McDade ,  John W. Simpson ,  Jan Berka ,  Christopher M. Colangelo

IPC: C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC classification number: C12Q1/6837 ,  B01L3/502715 ,  B01L9/527 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0877 ,  B82Y30/00 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/6452 ,  G01N2201/08 ,  G01N2201/0846 ,  C12Q2531/125 ,  C12Q2535/101 ,  C12Q2565/301 ,  C12Q2565/107 ,  C12Q2525/307

Abstract: Disclosed herein are methods and apparatuses for sequencing a nucleic acid. In one aspect, the method includes annealing a population of circular nucleic acid molecules to a plurality of anchor primers linked to a solid support, and amplifying those members of the population of circular nucleic acid molecules which anneal to the target nucleic acid, and then sequencing the amplified molecules by detecting the presence of a sequence byproduct such as pyrophosphate.

Abstract translation: 本文公开了用于测序核酸的方法和装置。 一方面，该方法包括将多个环状核酸分子退火至与固体支持物连接的多个锚定引物，并扩增与靶核酸退火的环状核酸分子群体的那些成员，然后测序 通过检测序列副产物如焦磷酸盐的存在来扩增分子。

公开(公告)号：US20040259091A1

公开(公告)日：2004-12-23

申请号：US10487089

申请日：2004-08-09

Inventor： Kenji Yasuda ,  Satoshi Takashi

IPC: C12Q001/68 ,  G01N021/64

CPC classification number: G01N21/76 ,  G01N21/253 ,  G01N2201/0846

Abstract: A luminescence microarray plate suitable for use in luminescent reaction, and a luminescence detecting device utilizing the microarray plate. A partition wall is provided around each microarray minute reaction region to prevent the entry of surrounding luminescence. Luminescence from each minute region is detected by a photoconducting guide. The amount of entry of surrounding luminescence can be reduced and improvements in accuracy and sensitivity can be achieved.

Abstract translation: 适用于发光反应的发光微阵列板和利用微阵列板的发光检测装置。 围绕每个微阵列微反应区域设置分隔壁以防止周围发光的进入。 通过光导引导来检测来自每个微小区域的发光。 可以减少周围发光的进入量，并且可以实现精度和灵敏度的提高。

公开(公告)号：US20030032039A1

公开(公告)日：2003-02-13

申请号：US10180647

申请日：2002-06-26

Applicant: SRU Biosystems, LLC

Inventor： Brian T. Cunningham ,  Peter Y. Li ,  Frank DeFilippi

IPC: C12Q001/68 ,  G01N033/53 ,  G01N033/542 ,  C12M001/34 ,  G01J003/42

CPC classification number: G01N21/253 ,  B01L3/5085 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/0829 ,  G01N21/4788 ,  G01N21/7743 ,  G01N33/54373 ,  G01N2201/0635 ,  G01N2201/08 ,  G01N2201/0833 ,  G01N2201/0846 ,  G01N2201/1248 ,  G02B5/1809 ,  G02B5/1814 ,  G02B21/06

Abstract: Method and apparatus for detecting biomolecular interactions. The use of labels is not required and the methods may be performed in a high-throughput manner. An apparatus for detecting biochemical interactions occurring on the surface of a biosensor includes a light source. A first optical fiber is coupled to the light source and illuminates the biosensor. A second optical fiber detects a wavelength reflected from the biosensor. A spectrometer determines spectra of a reflected signal from the biosensor.

Abstract translation: 用于检测生物分子相互作用的方法和装置。 不需要使用标签，并且可以以高通量的方式执行方法。 用于检测在生物传感器的表面上发生的生物化学相互作用的装置包括光源。 第一光纤耦合到光源并照亮生物传感器。 第二光纤检测从生物传感器反射的波长。 光谱仪确定来自生物传感器的反射信号的光谱。

公开(公告)号：US5862285A

公开(公告)日：1999-01-19

申请号：US511357

申请日：1995-08-04

Applicant: George L. Danielian ,  Wolfgang Neuberger

Inventor： George L. Danielian ,  Wolfgang Neuberger

IPC: G01N21/77 ,  G02B6/04 ,  G02B6/06 ,  C02B6/06

CPC classification number: G01N21/474 ,  G02B6/06 ,  G01N2021/4709 ,  G01N2021/4742 ,  G01N2201/0833 ,  G01N2201/0846 ,  G02B6/04

Abstract: Multifunctional optical fiber bundles for image and signal transmission or sensor applications are proposed. The optical fiber bundles exhibit reduced speckle noise resulting from spatial inhomogeneity and asymmetry of radiation of specific fibers of the bundle. This is achieved by ordering of fiber positions in the bundle sensitive probe tips according to some prescribed rule.

Abstract translation: 提出了用于图像和信号传输或传感器应用的多功能光纤束。 光纤束表现出由于束的特定纤维的辐射的空间不均匀性和不对称性而导致的散斑噪声减小。 这是通过根据一些规定的规则对束敏感探针尖端中的纤维位置的顺序来实现的。