公开(公告)号：US20210079333A1

公开(公告)日：2021-03-18

申请号：US17046264

申请日：2018-04-09

Applicant: CYFUSE BIOMEDICAL K.K.

Inventor： Kumika MATSUBAYASHI ,  Yasuto KISHII

IPC: C12M1/12 ,  C12M3/00 ,  C12M1/00 ,  C12M1/04 ,  C12M1/26 ,  C12N5/00

Abstract: The connection support device includes a rod-shaped member insertable into respective hollow portions of two or more cell structures, the rod-shaped member being inserted into the hollow portions, the rod-shaped member including a circular cross section having an outer diameter capable of adhering to inner surfaces of the cell structures when the rod-shaped member contracts after maturing, and a total length longer than a sum of respective lengths of the two or more cell structures, and two presser devices including clamp portions capable of being fixed to the rod-shaped member by clamping and fitting to the rod-shaped member, the rod-shaped member being made of a material with oxygen permeability.

公开(公告)号：US20170254741A1

公开(公告)日：2017-09-07

申请号：US15599765

申请日：2017-05-19

Applicant: Sumitomo Electric Industries, Ltd. ,  Cyfuse Biomedical K.K. ,  Kyoto Stem Cell Innovation, Inc.

Inventor： Hiroshi Suganuma ,  Takuya Okuno ,  Asako Motomura ,  Shizuka Akieda ,  Manami Tsuji ,  Yuna Okina ,  Yoko Sugiyama ,  Kazuhiro Aiba ,  Li Liu

IPC: G01N21/25 ,  G06F17/30 ,  G01N21/359 ,  G06N99/00 ,  G01N17/00 ,  G01N21/01

CPC classification number: G01N21/255 ,  G01N17/004 ,  G01N21/01 ,  G01N21/359 ,  G01N21/4738 ,  G01N2021/0106 ,  G01N2201/10 ,  G01N2201/129 ,  G06F16/583 ,  G06K9/00147 ,  G06K9/0055 ,  G06N20/00 ,  G06T2207/30024

Abstract: A quality evaluation method includes an acquisition step of acquiring spectral data related to transmitted light or diffusely reflected light from a cell mass by irradiating the cell mass with measurement light including near-infrared light, and an evaluation step of evaluating quality of the cell mass, based on the spectral data of the cell mass acquired in the acquisition step.

公开(公告)号：US20160348066A1

公开(公告)日：2016-12-01

申请号：US15114970

申请日：2015-09-25

Applicant: CYFUSE BIOMEDICAL K.K.

Inventor： Koji KUCHIISHI ,  Tadashi TAMURA

IPC: C12N5/00 ,  C12M1/34 ,  C12M1/32

CPC classification number: C12N5/0062 ,  C12M21/08 ,  C12M23/12 ,  C12M25/00 ,  C12M33/04 ,  C12M33/06 ,  C12M41/46 ,  C12N5/00 ,  C12N11/00

Abstract: Provided are: a cell tray provided with a concave part for supporting a cell aggregate and a hole formed on the bottom of the concave part; and a device for producing a cell structure, said device being provided with the cell tray and a puncture part passing through the cell tray and the cell aggregate, characterized in that the puncture part passes through the cell aggregate supported by the concave part until the tip thereof intrudes into the hole. Also provided is a system for producing a cell structure, said system comprising: a determination part that examines the characteristics of cell aggregates; a fractionation part that classifies the cell aggregates depending on the results of the examination by the determination part; a discharge part that disposes the cell aggregates in a cell tray depending on the results of the classification by the fractionation part; a puncture part that pass through a plurality of cell aggregates disposed in the cell tray; and a holding part that aligns and holds a plurality of puncture parts passing through a plurality of cell aggregates.

Abstract translation: 提供有：设置有用于支撑细胞骨架的凹部的细胞托盘和形成在凹部的底部上的孔; 以及用于制造电池结构的装置，所述装置设置有电池托盘和通过电池托盘和电池组件的穿刺部件，其特征在于，穿刺部件穿过由凹部支撑的电池组件，直到尖端 其侵入孔中。 还提供了一种用于生产细胞结构的系统，所述系统包括：检测细胞聚集体特征的确定部分; 分级部，其根据测定部的检查结果对细胞聚集进行分类; 排出部，其根据分级部的分类结果将细胞聚集体配置在细胞托盘中; 刺穿部分，其穿过设置在所述细胞托盘中的多个细胞聚集体; 以及保持部，其对齐并保持穿过多个细胞聚集体的多个穿刺部。

公开(公告)号：US20240303812A1

公开(公告)日：2024-09-12

申请号：US18599334

申请日：2024-03-08

Applicant: SCREEN Holdings Coo., Ltd. ,  Cyfuse Biomedical K.K.

Inventor： Ryo HASEBE ,  Toshihiko Maekawa ,  Ayu Inoue

IPC: G06T7/00

CPC classification number: G06T7/0012 ,  G06T2207/10101 ,  G06T2207/20081 ,  G06T2207/30024 ,  G06T2207/30056

Abstract: According to an evaluation method, first, a biological sample is taken an image, and an image in which intensity values are distributed is acquired. After that, a localization region corresponding to a fibrotic region is extracted from the taken image. At that time, a region of which intensity value satisfies a predetermined requirement in the taken image is extracted as the localization region. Alternatively, the taken image is input to a trained model created in advance, and a localization region output from the trained model is obtained. This makes it possible to noninvasively observe the fibrotic region of the biological sample, to evaluate the condition of the biological sample, without processing cells by staining or the like.

公开(公告)号：US12037573B2

公开(公告)日：2024-07-16

申请号：US17046114

申请日：2018-04-09

Applicant: CYFUSE BIOMEDICAL K.K.

Inventor： Satoshi Gojo ,  Daisuke Kami ,  Kumika Matsubayashi ,  Shizuka Akieda

IPC: C12M1/00 ,  C12M1/12

CPC classification number: C12M29/04 ,  C12M23/06 ,  C12M25/10

Abstract: It is solved by the rod-shaped tubular cell structure maintaining support device for cultivating a tubular cell structure including a hollow portion inside for a predetermined period of time while maintaining the hollow portion, the tubular cell structure maintaining support device including an outer diameter insertable into the hollow portion of the cell structure, and when inserted into the hollow portion, capable of allowing the tubular cell structure maintaining support device to adhere to an inner surface of the cell structure, and a penetration conduit including openings at both ends of the tubular cell structure maintaining support device, and penetrating between the openings, wherein the tubular cell structure maintaining support device has an oxygen permeable structure from the penetration conduit to an outer surface of the tubular cell structure maintaining support device.

公开(公告)号：US20220315899A1

公开(公告)日：2022-10-06

申请号：US17432029

申请日：2020-02-21

Applicant: CYFUSE BIOMEDICAL K.K.

Inventor： Toshihiko MAEKAWA ,  Eri NAGAO ,  Izumi IDE ,  Sakura KAJIYAMA

IPC: C12N5/071 ,  G01N33/50 ,  C12Q1/6883

Abstract: Long awaited is a human liver-like three-dimensional construct that makes it possible to carry out evaluation of human-specific toxicity and the like accurately and in a simple manner. The present invention provides a human liver-like three-dimensional construct comprising a heterospheroid, in which human hepatic cells and other human-derived cells which are not human hepatic cells are aggregated. This human liver-like three-dimensional construct is characterized in that the other human-derived cells are at least one selected from human hepatic stellate cells and the like, and the other human-derived cell to the human hepatic cell count ratio is at least 0.01 but less than 1.

公开(公告)号：US20210324319A1

公开(公告)日：2021-10-21

申请号：US16616284

申请日：2019-02-01

Applicant: CYFUSE BIOMEDICAL K.K.

Inventor： Yasuto KISHII ,  Norihiko TOKUNAGA

IPC: C12M1/26 ,  C12M3/00 ,  C12M1/32 ,  C12M1/00

Abstract: The cell structure manufacturing system including a cabinet being a closed space capable of maintaining an inside at a high cleanness, a cultivation plate supplying device configured to supply one cultivation plate of a plurality of cultivation plates to a conveying device in a cultivation plate supplying area, a transfer device including a cell aggregate injection device configured to discharge and transfer, and a sticking device including a needle-like body clamping mechanism configured to remove one needle-like body from a needle-like body supplying mechanism storing a plurality of needle-like bodies and perform gripping of the one needle-like body.

公开(公告)号：US20210032585A1

公开(公告)日：2021-02-04

申请号：US17046114

申请日：2018-04-09

Applicant: CYFUSE BIOMEDICAL K.K.

Inventor： Satoshi GOJO ,  Daisuke KAMI ,  Kumika MATSUBAYASHI ,  Shizuka AKIEDA

IPC: C12M1/00 ,  C12M1/12

Abstract: It is solved by the rod-shaped tubular cell structure maintaining support device for cultivating a tubular cell structure including a hollow portion inside for a predetermined period of time while maintaining the hollow portion, the tubular cell structure maintaining support device including an outer diameter insertable into the hollow portion of the cell structure, and when inserted into the hollow portion, capable of allowing the tubular cell structure maintaining support device to adhere to an inner surface of the cell structure, and a penetration conduit including openings at both ends of the tubular cell structure maintaining support device, and penetrating between the openings, wherein the tubular cell structure maintaining support device has an oxygen permeable structure from the penetration conduit to an outer surface of the tubular cell structure maintaining support device.

公开(公告)号：US20140283356A1

公开(公告)日：2014-09-25

申请号：US14358442

申请日：2012-11-16

Applicant: SAGA UNIVERSITY ,  CYFUSE BIOMEDICAL K.K.

Inventor： Koichi Nakayama

IPC: C12N5/00

CPC classification number: C12N5/0062 ,  C12M21/08 ,  C12M25/00 ,  C12M25/14 ,  C12M33/04 ,  Y10T29/49826 ,  Y10T29/53961

Abstract: A device for manufacturing a support for fixing cells of the present invention is capable of easily manufacturing a support that has needle-like bodies arranged on a base. The manufacturing device 2 is provided with a base 10, a holder 4, an insertion guide 6 and an alignment mechanism. The base 10 is retained by a holder 4. The base 10 is provided with a plurality of openings 15, into which needle-like bodies 20 are inserted and fixed. An insertion guide 6 is provided next to the holder 4 and includes slots 12 for placing a plurality of needle-like bodies 20 generally parallel to each other. The insertion guide 6 is provided with a plurality of slots 12 for placing the needle-like bodies 20 at corresponding positions to the openings 15, so that the alignment mechanism moves the insertion guide 6 such that the feed openings 12a are positioned adjacent to the openings 15. After positioning the feed openings 12a adjacent to the openings 15, the needle-like bodies 20 are introduced into the openings 15, whereby the needle-like bodies 20 are implanted into the base 10 to form a support.

Abstract translation: 用于制造本发明的固定单元的支撑体的装置能够容易地制造布置在基部上的具有针状物体的支撑体。 制造装置2设置有基部10，保持器4，插​​入引导件6和对准机构。 基座10由保持器4保持。基座10设置有多个开口15，针状体20插入并固定到开口15中。 插入引导件6设置在保持器4的旁边，并且包括用于放置大体上彼此平行的多个针状体20的槽12。 插入引导件6设置有多个狭缝12，用于将针状体20放置在与开口15相对应的位置处，使得对准机构移动插入引导件6，使得进给开口12a邻近开口 在将进给开口12a定位在与开口15相邻的位置之后，将针状体20引入到开口15中，由此将针状体20植入到基部10中以形成支撑体。