公开(公告)号：US20130115670A1

公开(公告)日：2013-05-09

申请号：US13261540

申请日：2011-06-15

Applicant: Nelson Nazareth ,  David Ward ,  David Edge

Inventor： Nelson Nazareth ,  David Ward ,  David Edge

IPC: C12N15/10

CPC classification number: C12N15/1003 ,  B01L3/5029 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/04 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2200/147 ,  B01L2300/044 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/049 ,  C12N1/066 ,  C12N1/08 ,  F25B21/04

Abstract: A process for the disruption of a biological cell comprising freezing, boiling or perhaps alternately freezing and boiling the material containing the biological cell using a thermoelectric cell a base face whereof is contiguous with a heat sink/source held at a substantially constant temperature and a working face . Apparatus for carrying out the disruption process comprises a peltier cell a base face of which is flexibly attached to a heat sink arranged to be kept at a constant temperature of around 50° C. and a working face of which is contiguous with a reaction vessel or a reaction vessel holder. Reversal of the voltage in the peltier cell enables the working face alternately to reach below freezing and above boiling temperatures, and/or with use of a resistive wire on the vessel holder for heating with the TEC used purely for cooling The peltier cell base face is constructed of materials which tend to inhibit disintegration of the peltier cell brought about by expansion and contraction under heat.

Abstract translation: 一种用于破坏生物细胞的方法，包括使用热电池冷冻，沸腾或者可能交替地冷冻和沸腾含有生物细胞的材料，其基面与保持在基本上恒定的温度的热沉/源连接，并且工作 脸。 用于进行破碎过程的装置包括一个珀耳帖细胞，其基面被柔性地附接到散热器，该散热器被设置成保持在约50℃的恒定温度，其工作面与反应容器相连， 反应容器支架。 在皮肤细胞中的电压反转使得工作面交替地达到低于冰点和沸点以上的温度，和/或使用电阻丝在容器保持器上用于纯化用于冷却的TEC加热珀耳帖细胞基面是 由倾向于抑制由加热引起的膨胀和收缩引起的珀耳帖细胞崩解的材料构成。

公开(公告)号：US4378465A

公开(公告)日：1983-03-29

申请号：US201833

申请日：1980-10-29

Applicant: David J. Green ,  John A. Gordon ,  David Edge

Inventor： David J. Green ,  John A. Gordon ,  David Edge

IPC: G01D5/12 ,  G01B7/00 ,  G01B7/004 ,  G06F3/033 ,  G06F3/046 ,  G08C21/00 ,  H04N1/00

CPC classification number: G06F3/046 ,  G01B7/004

Abstract: Apparatus is disclosed for generating a signal representative of a co-ordinate position of a movable indicating member, the member being constructed for interacting magnetically with sensing coils. The apparatus includes a plurality of sensing coils (2 to 11) for interacting magnetically with such a member, the coils being positioned substantially in or adjacent to a real or imaginary surface in or adjacent to which such an indicating member may be moved, the sensing coils being positioned to divide an area of interest in the surface into regions, each of the coils encircling part of the area of interest and having conductors in the area substantially along region boundaries only, each of the regions being within an arrangement of the sensing coils particular to that region. A further coil (1) is positioned substantially in or adjacent to the real or imaginary surface and surrounds the entire area of interest. There are a first one (2) and a second one (3) of the sensing coils which have conductors at substantially the same pitch but which coils are offset relative to each other. Processing means (16, D2 to D11, 17, G2 to G11 and L1 to L9) includes an exclusive-OR gate (15), an output of each of the first and second ones of the sensing coils being coupled only to a respective input of this gate. The processing means uses the output signal of the exclusive-OR gate and other signals derived from the sensing coils with reference to the signal in the further coil to produce a binary coded signal representative of a co-ordinate position of such a member.

Abstract translation: 公开了用于产生表示可移动指示构件的坐标位置的信号的装置，该构件被构造用于与感测线圈磁性相互作用。 该装置包括用于与这种构件磁性相互作用的多个感测线圈（2至11），线圈基本上位于或邻近这样的指示构件可移动的或邻近的实心或虚拟表面，感测 线圈被定位成将表面中的感兴趣区域分成区域，每个线圈环绕感兴趣区域的一部分，并且在该区域中仅具有基本上沿着区域边界的导体，每个区域在感测线圈的布置内 特别是该地区。 另外的线圈（1）基本上位于或邻近实际或虚拟表面并且围绕感兴趣的整个区域。 传感线圈有第一个（2）和第二个（3），这些传感线圈具有基本上相同间距的导体，但哪些线圈相对于彼此偏移。 处理装置（16，D2至D11,17，G2至G11和L1至L9）包括异或门（15），第一和第二感测线圈中的每一个的输出仅耦合到相应的输入 的这个门。 处理装置使用异或门的输出信号和从感测线圈导出的其他信号，参考另外的线圈中的信号，以产生代表这种部件的坐标位置的二进制编码信号。