公开(公告)号：US07855069B2

公开(公告)日：2010-12-21

申请号：US11253541

申请日：2005-10-19

Applicant: Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Kwang-ho Cheong ,  Chang-eun Yoo ,  Seung-yeon Yang

Inventor： Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Kwang-ho Cheong ,  Chang-eun Yoo ,  Seung-yeon Yang

IPC: C12M1/42 ,  C12M1/33

CPC classification number: C12M47/06 ,  B01F11/0266 ,  B01F13/0059 ,  B01L3/502761 ,  B01L2300/0816 ,  C12N1/066 ,  C12N13/00

Abstract: A method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser are provided. According to the method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.

Abstract translation: 提供了使用微磁珠和激光快速破坏细胞或病毒的方法和装置。 根据使用微磁珠和激光快速破坏细胞或病毒的方法和装置，可以在40秒内进行细胞裂解，使用激光二极管可以使装置小型化，DNA纯化步骤可以直接在 细胞或病毒的破坏以及含有DNA的溶液可以转移到随后的步骤中，随后的反应的抑制剂与细胞碎片和珠子一起用电磁铁去除。 另外，通过细胞裂解芯片，解决了蒸发问题，可以通过磁珠将振动有效地转移到细胞中，通过疏水处理芯片的内表面和细胞来解决粗糙表面上的微流体问题 裂解芯片可应用于LOC。

公开(公告)号：US20090322354A1

公开(公告)日：2009-12-31

申请号：US11695905

申请日：2007-04-03

Applicant: Kyu-tae YOO ,  Kyu-sang LEE ,  Won-seok CHUNG ,  Jeo-young SHIM ,  Yeon-ja CHO

Inventor： Kyu-tae YOO ,  Kyu-sang LEE ,  Won-seok CHUNG ,  Jeo-young SHIM ,  Yeon-ja CHO

IPC: G01R27/08

CPC classification number: G01N33/54373

Abstract: A method of detecting a presence of bio-molecules, or a concentration of the target bio-molecules using a field effect transistor, includes allowing a first sample including a first target bio-molecule to contact a sensing surface of the field effect transistor and measuring a change in an electric signal of the field effect transistor, the field effect transistor including a substrate, a source region and a drain region, the source region and the drain region formed apart from each other on the substrate, the source region and the drain region each doped to having an opposite polarity than a polarity of the substrate, a channel region disposed between the source region and the drain region and an insulating layer including the sensing surface, the insulating layer disposed on the channel region.

Abstract translation: 使用场效应晶体管检测生物分子的存在或靶生物分子的浓度的方法包括允许包含第一靶生物分子的第一样品接触场效应晶体管的感测表面并测量 场效应晶体管的电信号的变化，所述场效应晶体管包括基板，源极区和漏极区，在所述基板上彼此分离形成的所述源极区和漏极区，所述源极区和漏极 每个被掺杂为具有与衬底的极性相反的极性，设置在源区和漏区之间的沟道区和包括感测表面的绝缘层，绝缘层设置在沟道区上。

公开(公告)号：US20070251301A1

公开(公告)日：2007-11-01

申请号：US11619650

申请日：2007-01-04

Applicant: Kyu-sang LEE ,  Kyu-tae YOO ,  Jeo-young SHIM ,  Won-seok CHUNG ,  Yeon-ja CHO

Inventor： Kyu-sang LEE ,  Kyu-tae YOO ,  Jeo-young SHIM ,  Won-seok CHUNG ,  Yeon-ja CHO

IPC: G01N11/00

CPC classification number: G01N27/4145 ,  G01N27/27

Abstract: A method for simultaneously detecting a size and concentration of ionic materials includes measuring voltage drop values of at least three ionic materials of which sizes and concentrations are known using each of at least two FET-based sensors having different electrical characteristics, determining at least three points in a three-dimensional plot from the known sizes, concentrations and the measured voltage drop values, approximating the at least three points into a single plane, measuring a voltage drop value of an ionic material of which size and concentration are unknown using the at least two FET-based sensors, determining equipotential lines existing on the plane using the voltage drop value of the unknown ionic material and determining a cross point between each of the equipotential lines.

Abstract translation: 用于同时检测离子材料的尺寸和浓度的方法包括测量至少三种离子材料的电压降值，其中尺寸和浓度已知使用具有不同电特性的至少两个基于FET的传感器中的每一个，确定至少三个点 在已知尺寸，浓度和测量的电压降值的三维图中，将至少三个点近似为单个平面，测量其尺寸和浓度未知的离子材料的电压降值，至少使用至少 两个基于FET的传感器，使用未知离子材料的电压降值确定在平面上存在的等电位线，并确定每个等电位线之间的交叉点。

公开(公告)号：US20070159216A1

公开(公告)日：2007-07-12

申请号：US11621191

申请日：2007-01-09

Applicant: Kyu-sang LEE ,  Kyu-tae YOO ,  Jeo-young SHIM ,  Jin-tae KIM ,  Yeon-ja CHO

Inventor： Kyu-sang LEE ,  Kyu-tae YOO ,  Jeo-young SHIM ,  Jin-tae KIM ,  Yeon-ja CHO

IPC: H03F3/45

CPC classification number: G01N33/54373 ,  C12Q1/6825 ,  G01N27/4145

Abstract: Provided are a FET-based sensor for detecting an ionic material, an ionic material detecting device including the FET-based sensor, and a method of detecting an ionic material using the FET-based sensor. The FET-based sensor includes: a sensing chamber including a reference electrode and a plurality of sensing FETs; and a reference chamber including a reference electrode and a plurality of reference FETs. The method includes: flowing a first solution into and out of the sensing chamber and the reference chamber of the FET-based sensor; flowing a second solution expected to contain an ionic material into and out of the sensing chamber while continuously flowing the first solution into and out of the reference chamber; measuring a current in a channel region between the source and drain of each of the sensing and reference FETs; and correcting the current of the sensing FETs.

Abstract translation: 提供了一种用于检测离子材料的基于FET的传感器，包括基于FET的传感器的离子材料检测装置以及使用基于FET的传感器检测离子材料的方法。 基于FET的传感器包括：感测室，包括参考电极和多个感测FET; 以及包括参考电极和多个参考FET的参考室。 该方法包括：使第一溶液流入和流出基于FET的传感器的感测室和参考室; 使预期含有离子材料的第二溶液流入和流出感测室，同时使第一溶液持续流入和移出参考室; 测量每个感测和参考FET的源极和漏极之间的沟道区域中的电流; 并校正感测FET的电流。

公开(公告)号：US07943970B2

公开(公告)日：2011-05-17

申请号：US11695886

申请日：2007-04-03

Applicant: Kyu-tae Yoo ,  Kyu-sang Lee ,  Jeo-young Shim ,  Won-seok Chung ,  Yeon-ja Cho

Inventor： Kyu-tae Yoo ,  Kyu-sang Lee ,  Jeo-young Shim ,  Won-seok Chung ,  Yeon-ja Cho

IPC: G01N27/403

CPC classification number: G01N33/54373 ,  G01N27/4145

Abstract: Provided is a method of detecting the presence of a target bio-molecule or a concentration of the bio-molecule using a field effect transistor. The method includes: contacting a first sample having a first target bio-molecule with a reference electrode of a field effect transistor; measuring a first electric signal change of the field effect transistor; contacting a second sample with a sensing surface of the same field effect transistor; measuring a second electric signal change of the field effect transistor; and comparing the first electric signal with the second electric signal.

Abstract translation: 提供了使用场效应晶体管检测目标生物分子的存在或生物分子的浓度的方法。 该方法包括：将具有第一目标生物分子的第一样品与场效应晶体管的参比电极接触; 测量场效应晶体管的第一电信号变化; 使第二样品与相同场效应晶体管的感测表面接触; 测量场效应晶体管的第二电信号变化; 以及将所述第一电信号与所述第二电信号进行比较。

公开(公告)号：US20080199930A1

公开(公告)日：2008-08-21

申请号：US12031947

申请日：2008-02-15

Applicant: Jeong-gun LEE ,  Young-nam KWON ,  Young-a KIM ,  Myo-yong LEE ,  Shin-i YOO ,  Yeon-ja CHO ,  Kwang-ho CHEONG ,  Chang-eun YOO ,  Seung-yeon YANG

Inventor： Jeong-gun LEE ,  Young-nam KWON ,  Young-a KIM ,  Myo-yong LEE ,  Shin-i YOO ,  Yeon-ja CHO ,  Kwang-ho CHEONG ,  Chang-eun YOO ,  Seung-yeon YANG

IPC: C12N13/00 ,  C12M1/00

CPC classification number: C12M47/06 ,  B01F11/0266 ,  B01F13/0059 ,  B01L3/502761 ,  B01L2300/0816 ,  C12N1/066 ,  C12N13/00

Abstract: A method and apparatus for rapid disruption of cells or viruses using beads and a laser are provided. According to the method and apparatus for rapid disruption of cells or viruses using beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.

Abstract translation: 提供了使用珠和激光来快速破坏细胞或病毒的方法和装置。 根据使用珠粒和激光快速破坏细胞或病毒的方法和装置，可以在40秒内进行细胞裂解，可以使用激光二极管使装置小型化，DNA纯化步骤可以在细胞破碎后直接进行 或病毒，并且含有DNA的溶液可以转移到随后的步骤中，在用电磁体除去后续反应的抑制剂附着的细胞碎片和珠粒之后。 另外，通过细胞裂解芯片，解决了蒸发问题，可以通过磁珠有效地将振动传递到细胞，粗糙表面上的微流体问题通过疏水处理芯片的内表面和电池来解决 裂解芯片可应用于LOC。

公开(公告)号：US20070235760A1

公开(公告)日：2007-10-11

申请号：US11733539

申请日：2007-04-10

Applicant: Jeo-young Shim ,  Kyu-tae Yoo ,  Kyu-sang Lee ,  Won-seok Chung ,  Yeon-ja Cho ,  Chang-eun Yoo

Inventor： Jeo-young Shim ,  Kyu-tae Yoo ,  Kyu-sang Lee ,  Won-seok Chung ,  Yeon-ja Cho ,  Chang-eun Yoo

IPC: H01L31/00

CPC classification number: H01L29/78 ,  G01N27/4145

Abstract: A field effect transistor for detecting an analyte having a thiol group includes a substrate, a source region and a drain region formed apart from each other on the substrate, the source region and the drain region being doped such that a polarity of the source and drain region is opposite to a polarity of the substrate, a channel region disposed between the source region and the drain region, an insulating layer formed of an electrically insulating material and disposed on the channel region, a gold layer disposed on the insulating layer and a reference electrode disposed apart from the gold layer.

Abstract translation: 用于检测具有硫醇基的分析物的场效应晶体管包括在衬底上彼此分开形成的衬底，源极区和漏极区，源极区和漏极区被掺杂，使得源极和漏极的极性 区域与基板的极性相反，设置在源极区域和漏极区域之间的沟道区域，由电绝缘材料形成并设置在沟道区域上的绝缘层，设置在绝缘层上的金层和参考 电极与金层分开设置。

公开(公告)号：US20070231211A1

公开(公告)日：2007-10-04

申请号：US11695886

申请日：2007-04-03

Applicant: Kyu-tae Yoo ,  Kyu-sang Lee ,  Jeo-young Shim ,  Won-seok Chung ,  Yeon-ja Cho

Inventor： Kyu-tae Yoo ,  Kyu-sang Lee ,  Jeo-young Shim ,  Won-seok Chung ,  Yeon-ja Cho

IPC: G01N30/96

CPC classification number: G01N33/54373 ,  G01N27/4145

Abstract: Provided is a method of detecting the presence of a target bio-molecule or a concentration of the bio-molecule using a field effect transistor. The method includes: contacting a first sample having a first target bio-molecule with a reference electrode of a field effect transistor; measuring a first electric signal change of the field effect transistor; contacting a second sample with a sensing surface of the same field effect transistor; measuring a second electric signal change of the field effect transistor; and comparing the first electric signal with the second electric signal.

Abstract translation: 提供了使用场效应晶体管检测目标生物分子的存在或生物分子的浓度的方法。 该方法包括：将具有第一目标生物分子的第一样品与场效应晶体管的参比电极接触; 测量场效应晶体管的第一电信号变化; 使第二样品与相同场效应晶体管的感测表面接触; 测量场效应晶体管的第二电信号变化; 以及将所述第一电信号与所述第二电信号进行比较。

公开(公告)号：US20060264620A1

公开(公告)日：2006-11-23

申请号：US11345174

申请日：2006-02-01

Applicant: Myo-yong Lee ,  Joong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Yeon-ja Cho ,  Shin-i Yoo

Inventor： Myo-yong Lee ,  Joong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Yeon-ja Cho ,  Shin-i Yoo

IPC: C07H21/04

CPC classification number: C07H21/04 ,  C12N15/1006

Abstract: Provided are methods of isolating and amplifying nucleic acids from and in a nucleic acid-containing sample. The nucleic acid isolation method includes contacting a nucleic acid-containing sample to a silanized solid support to capture nucleic acids to the silanized solid support and treating the nucleic acid-captured solid support with an alkaline solution of pH 9 to 14. The nucleic acid amplification method includes contacting a nucleic acid-containing sample to a silanized solid support to capture nucleic acids to the silanized solid support; treating the nucleic acid-captured solid support with an alkaline solution of pH 9 to 14; and adding a nucleic acid amplification solution to the resultant solution after the alkaline solution treatment to perform nucleic acid amplification.

Abstract translation: 提供从含核酸样品中分离和扩增核酸的方法。 核酸分离方法包括将含核酸的样品与硅烷化的固体支持物接触以将核酸捕获到硅烷化的固体支持物，并用pH9至14的碱性溶液处理核酸捕获的固体支持物。核酸扩增 方法包括将含核酸的样品与硅烷化的固体支持物接触以将核酸捕获到硅烷化固体支持物; 用pH9至14的碱性溶液处理核酸捕获的固体支持物; 并在碱溶液处理后向所得溶液中加入核酸扩增溶液进行核酸扩增。