公开(公告)号：US08993340B2

公开(公告)日：2015-03-31

申请号：US13051251

申请日：2011-03-18

Applicant: Hun-joo Lee ,  Jeong-gun Lee ,  Mi-jeong Song ,  Jong-myeon Park ,  Tae-seok Sim ,  Min-seok S. Kim

Inventor： Hun-joo Lee ,  Jeong-gun Lee ,  Mi-jeong Song ,  Jong-myeon Park ,  Tae-seok Sim ,  Min-seok S. Kim

IPC: G01N1/28 ,  C12N5/00 ,  C12M1/00 ,  B01L3/00 ,  B03C1/14 ,  G01N1/40 ,  C12M1/26 ,  B03C1/02

CPC classification number: C12M47/04 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2300/0864 ,  B03C1/02 ,  B03C1/14 ,  B03C1/145 ,  C12M1/266 ,  G01N1/4077 ,  Y10T436/25

Abstract: A cell separation device includes a channel or chamber in which a sample flows or moves, the sample including target cells marked with magnetic particles, and non-target cells, and a magnet which generates a magnetic first force in a first direction with respect to the sample within the channel or chamber. The channel or chamber of the cell separation device is applied with a second force in a second direction opposite to the first direction of the magnetic force. According to the cell separation device and a method of separating cells, the target cells move in the first direction by the magnetic force, and the non-target cells move in the second direction by the second force, by simultaneously applying the magnetic force and the second force in opposing directions, thereby separating the target cells from the non-target cells.

Abstract translation: 细胞分离装置包括样品流动或移动的通道或室，包括标记有磁性颗粒的靶细胞的样品和非靶细胞，以及相对于第一方向产生第一方向的磁性第一力的磁体 样品在通道或室内。 细胞分离装置的通道或腔室以与磁力的第一方向相反的第二方向施加第二力。 根据细胞分离装置和分离细胞的方法，靶细胞通过磁力在第一方向上移动，非靶细胞通过第二力在第二方向上移动，同时施加磁力和 从而将目标细胞与非靶细胞分离。

公开(公告)号：US08945687B2

公开(公告)日：2015-02-03

申请号：US13270790

申请日：2011-10-11

Applicant: Seonmi Yoon ,  Jaeyoung Choi ,  Hyeon Jin Shin ,  Jeong Gun Lee ,  Jongmyeon Park

Inventor： Seonmi Yoon ,  Jaeyoung Choi ,  Hyeon Jin Shin ,  Jeong Gun Lee ,  Jongmyeon Park

IPC: B05D3/06 ,  B29C59/16 ,  H05B6/00 ,  C09K5/14 ,  C03C17/25 ,  C23C18/14 ,  C03C17/00 ,  C08J7/06 ,  B29C71/02

CPC classification number: C09K5/14 ,  C03C17/006 ,  C03C17/256 ,  C03C2217/42 ,  C08J7/06 ,  C08J7/08 ,  C23C18/14 ,  Y02E10/50 ,  Y10T428/24355 ,  Y10T428/24372 ,  Y10T428/24802 ,  Y10T428/24909 ,  Y10T428/31678

Abstract: Disclosed are a heat transfer medium and a heat transfer method that uses the heat transfer medium. The heat transfer medium comprises a light-transparent substrate coated with a plurality of nano particles. The nano particles absorb light incident thereon to thereby produce heat, which is transferred to a target object to be heated. Nano particles can be applied onto a target object. After heating, the particles are removed by etching. Nano particles can be selectively applied to the light-transparent substrate or directly to a target object to be heat so as to localize heat-production and thus heat selective portions of the target object.

Abstract translation: 公开了使用传热介质的传热介质和传热方法。 传热介质包括涂覆有多个纳米颗粒的透光基板。 纳米颗粒吸收入射在其上的光，从而产生热量，其被传送到待加热的目标物体。 纳米颗粒可以施加到目标物体上。 加热后，通过蚀刻除去颗粒。 可以将纳米颗粒选择性地施加到光透明基板上，或者直接加到待加热的目标物体上，从而定位目标物体的热产生和热选择性部分。

公开(公告)号：US08753870B2

公开(公告)日：2014-06-17

申请号：US13605787

申请日：2012-09-06

Applicant: Hun-joo Lee ,  Jeong-gun Lee ,  Jeong-woo Choi ,  Yeon-jeong Kim ,  Jong-myeon Park ,  Mi-jeong Song ,  Jin-mi Oh

Inventor： Hun-joo Lee ,  Jeong-gun Lee ,  Jeong-woo Choi ,  Yeon-jeong Kim ,  Jong-myeon Park ,  Mi-jeong Song ,  Jin-mi Oh

IPC: C12M1/00 ,  C12Q1/68 ,  G01N33/53 ,  C12M1/34 ,  C12M3/00 ,  G01N30/96 ,  C07H21/02

CPC classification number: G01N33/54306 ,  G01N33/54366

Abstract: A device, system, and method for detecting or separating target molecules allowing efficient detection even when only a small amount of target molecules or target cells are included in a sample involving the use of a target molecule linkage portion, a signal production portion, and first and second separation portions.

Abstract translation: 用于检测或分离目标分子的装置，系统和方法，即使当仅使用少量靶分子或靶细胞包括使用靶分子连接部分，信号产生部分和第一 和第二分离部。

公开(公告)号：US08597572B2

公开(公告)日：2013-12-03

申请号：US12128680

申请日：2008-05-29

Applicant: Su-hyeon Kim ,  Yoon-kyoung Cho ,  Jeong-gun Lee

Inventor： Su-hyeon Kim ,  Yoon-kyoung Cho ,  Jeong-gun Lee

IPC: G01N33/50

CPC classification number: G01N35/00732 ,  G01N35/00069 ,  G01N35/08 ,  G01N2035/00752 ,  Y10T436/11

Abstract: Provided are an automatic analyzing method and apparatus for a microfludic system. The apparatus includes means for recognizing a disc or chip in the microfludic system, performs an operation and detection by automatically loading a DB according to a recognized type, and perform quantization of a test.

Abstract translation: 本发明提供一种微流体系统的自动分析方法和装置。 该装置包括用于识别微流体系统中的盘或芯片的装置，通过根据识别的类型自动加载DB执行操作和检测，并执行测试的量化。

公开(公告)号：US20130178543A1

公开(公告)日：2013-07-11

申请号：US13594610

申请日：2012-08-24

Applicant: Yeon-jeong KIM ,  You-sun KIM ,  Jeong-gun LEE ,  Sang-hyun BAEK ,  Jin-mi OH ,  Hyo-young JEONG

Inventor： Yeon-jeong KIM ,  You-sun KIM ,  Jeong-gun LEE ,  Sang-hyun BAEK ,  Jin-mi OH ,  Hyo-young JEONG

IPC: G01N33/574 ,  C12N5/095 ,  C40B30/04 ,  C07K17/00 ,  A61P35/00 ,  A61P35/04 ,  C12N5/09 ,  G01N33/577

CPC classification number: C07K14/705 ,  C12Q1/6881 ,  C12Q1/6886 ,  C12Q2600/158 ,  G01N33/57484

Abstract: A method of detecting a cancer stem cell or circulating tumor cell that has undergone epithelial-mesenchymal transition, comprising determining the level of caveolin-1 expressed by a sample cell, and comparing the level of caveolin-1 expressed by the sample cell to a control, wherein higher expression of caveolin-1 by the sample cell indicates that the sample cell is a cancer cell, as well as a method of detecting cancer or metastasis in a subject, and related methods and compositions.

Abstract translation: 一种检测已经经历上皮 - 间质转化的癌症干细胞或循环肿瘤细胞的方法，包括测定由样品细胞表达的窖蛋白-1的水平，并将由样品细胞表达的窖蛋白-1的水平与对照组 其中样品细胞中较高表达的小窝蛋白-1表明样品细胞是癌细胞，以及检测受试者的癌症或转移的方法，以及相关的方法和组合物。

公开(公告)号：US08469492B2

公开(公告)日：2013-06-25

申请号：US12620856

申请日：2009-11-18

Applicant: Beom-seok Lee ,  Jeong-gun Lee ,  In-seok Kang ,  Jin-seok Hong ,  Dustin Moon

Inventor： Beom-seok Lee ,  Jeong-gun Lee ,  In-seok Kang ,  Jin-seok Hong ,  Dustin Moon

IPC: B41J2/06

CPC classification number: B41J2/06 ,  B41J2/14

Abstract: A method of printing droplets using capillary electric charge concentration includes: providing a capillary nozzle comprising a back-end part and a front-end part disposed substantially opposite the back-end part; spacing a target member apart from the front-end part of the capillary nozzle at a predetermined distance; immersing the back-end part in a solution; and supplying a voltage to the solution. The back-end part transmits the solution to the front-end part.

Abstract translation: 使用毛细管电荷浓度印刷液滴的方法包括：提供包括后端部分和基本上与后端部分相对设置的前端部分的毛细管喷嘴; 将目标构件与毛细管喷嘴的前端部隔开预定距离; 将后端部分浸入溶液中; 并向该溶液提供电压。 后端部分将解决方案传输到前端部分。

公开(公告)号：US20130149774A1

公开(公告)日：2013-06-13

申请号：US13615267

申请日：2012-09-13

Applicant: Min-seok KIM ,  Tae-seok SIM ,  Yeon-jeong KIM ,  Sang-hyun BAEK ,  Jin-mi OH ,  Hyo-young JEONG ,  Jeong-gun LEE

Inventor： Min-seok KIM ,  Tae-seok SIM ,  Yeon-jeong KIM ,  Sang-hyun BAEK ,  Jin-mi OH ,  Hyo-young JEONG ,  Jeong-gun LEE

IPC: G01N27/02 ,  C12M1/36

CPC classification number: G01N15/12 ,  C12M41/36 ,  G01N2015/0065 ,  G01N2015/1081

Abstract: A cell counter system includes an inlet via which a fluid containing a plurality of cells inflows; a channel in which the fluid flows; a valve unit, which controls flow of the fluid in the channel; an electrode unit, which is arranged in the channel for measuring impedance for counting a number of the plurality of cells; and an outlet, which is connected to the channel to drain the fluid.

Abstract translation: 细胞计数器系统包括通过其流入含有多个细胞的流体入口的入口; 流体流动的通道; 阀单元，其控制流体在通道中的流动; 电极单元，布置在通道中用于测量用于对多个单元的数量进行计数的阻抗; 和出口，其连接到通道以排出流体。

公开(公告)号：US20130137092A1

公开(公告)日：2013-05-30

申请号：US13605787

申请日：2012-09-06

Applicant: Hun-joo LEE ,  Jeong-gun LEE ,  Jeong-woo CHOI ,  Yeon-jeong KIM ,  Jong-myeon PARK ,  Mi-jeong SONG ,  Jin-mi OH

Inventor： Hun-joo LEE ,  Jeong-gun LEE ,  Jeong-woo CHOI ,  Yeon-jeong KIM ,  Jong-myeon PARK ,  Mi-jeong SONG ,  Jin-mi OH

IPC: C12M1/34 ,  G01N33/566

CPC classification number: G01N33/54306 ,  G01N33/54366

Abstract: A device, system, and method for detecting or separating target molecules allowing efficient detection even when only a small amount of target molecules or target cells are included in a sample involving the use of a target molecule linkage portion, a signal production portion, and first and second separation portions.

Abstract translation: 用于检测或分离目标分子的装置，系统和方法，即使当仅使用少量靶分子或靶细胞包括使用靶分子连接部分，信号产生部分和第一 和第二分离部。

公开(公告)号：US08420026B2

公开(公告)日：2013-04-16

申请号：US13594293

申请日：2012-08-24

Applicant: Yoon-kyoung Cho ,  Jeong-gun Lee ,  Beom-seok Lee ,  Jong-myeon Park

Inventor： Yoon-kyoung Cho ,  Jeong-gun Lee ,  Beom-seok Lee ,  Jong-myeon Park

IPC: B04B13/00

CPC classification number: B01L3/502738 ,  B01F13/0059 ,  B01F15/0201 ,  B01F15/0233 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2300/0806 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0409 ,  B01L2400/0677 ,  F16K99/0001 ,  F16K99/0017 ,  F16K99/0019 ,  F16K99/0032 ,  F16K99/004 ,  F16K2099/0076 ,  F16K2099/0084

Abstract: A centrifugal force-based microfluidic device for nucleic acid extraction and a microfluidic system are provided. The microfluidic device includes a body of revolution; a microfluidic structure disposed in the body of revolution, the microfluidic structure including a plurality of chambers, channels connecting the chambers, and valves disposed in the channels to control fluid flow, the microfluidic structure transmitting the fluid using centrifugal force due to rotation of the body of revolution; and magnetic beads contained in one of the chambers which collect a target material from a biomaterial sample flowing into the chamber, wherein the microfluidic structure washes the magnetic beads which collect the target material, and separates nucleic acid by electromagnetic wave irradiation from an external energy source to the magnetic beads. The microfluidic system includes the microfluidic device; a rotation operating unit which rotates the body of revolution; and an external energy source which irradiates electromagnetic waves.

Abstract translation: 提供了用于核酸提取的离心力微流体装置和微流体系统。 微流体装置包括一个革命体; 设置在旋转体内的微流体结构，所述微流体结构包括多个室，连接所述室的通道和设置在通道中的阀以控制流体流动，所述微流体结构由于所述体的旋转而使用离心力传递流体 的革命 以及包含在其中一个室中的磁珠，其从流入腔室的生物材料样品收集目标材料，其中微流体结构洗涤收集目标材料的磁珠，并通过来自外部能量源的电磁波照射分离核酸 到磁珠。 微流体系统包括微流体装置; 使旋转体旋转的旋转操作单元; 以及照射电磁波的外部能量源。

公开(公告)号：US20130086998A1

公开(公告)日：2013-04-11

申请号：US13571202

申请日：2012-08-09

Applicant: June-young LEE ,  Sang-hyun BAEK ,  Hyo-young JEONG ,  Tae-seok SIM ,  Jeong-gun LEE

Inventor： June-young LEE ,  Sang-hyun BAEK ,  Hyo-young JEONG ,  Tae-seok SIM ,  Jeong-gun LEE

IPC: G01N1/20

CPC classification number: B01L3/502753 ,  B01L3/5027 ,  B01L2200/0621 ,  B01L2200/141 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0487 ,  G01N2035/00237 ,  G01N2035/0437 ,  Y10T137/4259

Abstract: A fluid controlling apparatus including at least one sample chamber for holding a fluid containing target materials; a cleaning chamber for holding a cleaning solution; a first multi-port connected to the at least one sample chamber through a first channel and connected to the cleaning chamber through a second channel; a filter portion, connected to the first multi-port through a third channel, for filtering the target materials; and a first pump, connected to the filter portion, for applying a pressure; and a method of controlling a fluid using the fluid controlling apparatus, which comprises passing the fluid containing the target materials from the at least one sample chamber to the filter portion; and cleaning a path of the fluid by passing the cleaning solution through the path.

Abstract translation: 一种流体控制装置，包括用于保持含有目标材料的流体的至少一个样品室; 用于保持清洁溶液的清洁室; 第一多端口，其通过第一通道连接到所述至少一个样品室，并通过第二通道连接到所述清洁室; 过滤器部分，通过第三通道连接到第一多端口，用于过滤目标材料; 以及连接到过滤器部分的用于施加压力的第一泵; 以及使用所述流体控制装置控制流体的方法，所述方法包括将包含所述目标材料的流体从所述至少一个样品室传送到所述过滤器部分; 以及通过使清洁溶液通过路径来清洁流体的路径。