公开(公告)号：US20180188230A1

公开(公告)日：2018-07-05

申请号：US15724213

申请日：2017-10-03

Applicant: ABBOTT LABORATORIES

Inventor： Jeffrey B. Huff ,  Mark A. Hayden ,  Peter J. Karabatsos ,  Andrew S. Schapals ,  Anthony S. Muerhoff ,  Felicia Bogdan ,  Thomas Leary ,  Shelley R. Holets-McCormack ,  Sophie Laurenson ,  Andrew T. Fischer ,  Richard Haack ,  Stefan Hershberger ,  Dustin House ,  Lei Qiao ,  M. Shawn Murphy ,  Mark R. Pope ,  Edna M. Prieto-Ballengee ,  QiaoQiao Ruan ,  Pathik Soni ,  Sergey Tetin ,  Lyle Yarnell

IPC: G01N33/487 ,  G01N33/543 ,  B01L3/00

CPC classification number: G01N33/48721 ,  B01L3/502761 ,  B01L2300/0645 ,  B01L2400/0406 ,  G01N33/5308 ,  G01N33/54306 ,  G01N2458/10

Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.