公开(公告)号：US20200256843A1

公开(公告)日：2020-08-13

申请号：US16864672

申请日：2020-05-01

Applicant: ABBOTT LABORATORIES

Inventor： Jeffrey B. Huff ,  Mark A. Hayden ,  Peter J. Karabatsos ,  Andrew S. Schapals ,  Anthony S. Muerhoff ,  M. Felicia Bogdan ,  Thomas Leary ,  Shelley R. Holets-McCormack ,  Sophie Laurenson ,  Andrew T. Fischer ,  Richard Haack ,  Stefan Hershberger ,  Dustin House ,  Lei Qiao ,  M. Shawn Murphy ,  Mark R. Pope ,  Edna M. Prieto-Ballengee ,  QiaoQiao Ruan ,  Pathik Soni ,  Sergey Tetin ,  Lyle Yarnell

IPC: G01N33/487 ,  G01N33/53 ,  B01L3/00 ,  G01N33/543

Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.

公开(公告)号：US20200209250A1

公开(公告)日：2020-07-02

申请号：US16729067

申请日：2019-12-27

Applicant: Abbott Laboratories

Inventor： Qiaoqiao Ruan ,  Patrick J. Macdonald ,  Kerry M. Swift ,  Sergey Y. Tetin ,  Brenda Calfin ,  Zhen Lin ,  Richard Haack ,  Mark R. Pope ,  John Prostko ,  Xiaoxing Qiu ,  Felicia Bogdan

IPC: G01N33/58 ,  G01N15/14 ,  G01N33/543

Abstract: The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a cross-linked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provided are methods of analyzing two or more analytes of interest in a biological sample in a single assay using microparticles and detection conjugates comprising different fluorophore labels, acquiring transmitted light and fluorescent images of the microparticles, and using a customized image analysis process to analyze the acquired images.

公开(公告)号：US20240377405A1

公开(公告)日：2024-11-14

申请号：US18761492

申请日：2024-07-02

Applicant: Abbott Laboratories

Inventor： Qiaoqiao Ruan ,  Patrick J. Macdonald ,  Kerry M. Swift ,  Sergey Y. Tetin ,  Brenda Calfin ,  Zhen Lin ,  Richard Haack ,  Mark R. Pope ,  John Prostko ,  Xiaoxing Qiu ,  M. Felicia Bogdan

IPC: G01N33/58 ,  G01N15/10 ,  G01N15/14 ,  G01N33/543

Abstract: The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a cross-linked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provided are methods of analyzing two or more analytes of interest in a biological sample in a single assay using microparticles and detection conjugates comprising different fluorophore labels, acquiring transmitted light and fluorescent images of the microparticles, and using a customized image analysis process to analyze the acquired images.

公开(公告)号：US12061200B2

公开(公告)日：2024-08-13

申请号：US16729067

申请日：2019-12-27

Applicant: Abbott Laboratories

Inventor： Qiaoqiao Ruan ,  Patrick J. Macdonald ,  Kerry M. Swift ,  Sergey Y. Tetin ,  Brenda Calfin ,  Zhen Lin ,  Richard Haack ,  Mark R. Pope ,  John Prostko ,  Xiaoxing Qiu ,  M. Felicia Bogdan

IPC: G01N33/53 ,  G01N15/14 ,  G01N33/543 ,  G01N33/58 ,  G01N15/10

CPC classification number: G01N33/582 ,  G01N15/14 ,  G01N33/54346 ,  G01N33/587 ,  G01N2015/1018

Abstract: The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a cross-linked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provided are methods of analyzing two or more analytes of interest in a biological sample in a single assay using microparticles and detection conjugates comprising different fluorophore labels, acquiring transmitted light and fluorescent images of the microparticles, and using a customized image analysis process to analyze the acquired images.

公开(公告)号：US11022598B2

公开(公告)日：2021-06-01

申请号：US15724213

申请日：2017-10-03

Applicant: ABBOTT LABORATORIES

Inventor： Jeffrey B. Huff ,  Mark A. Hayden ,  Peter J. Karabatsos ,  Andrew S. Schapals ,  Anthony S. Muerhoff ,  M. Felicia Bogdan ,  Thomas Leary ,  Shelley R. Holets-McCormack ,  Sophie Laurenson ,  Andrew T. Fischer ,  Richard Haack ,  Stefan Hershberger ,  Dustin House ,  Lei Qiao ,  M. Shawn Murphy ,  Mark R. Pope ,  Edna M. Prieto-Ballengee ,  QiaoQiao Ruan ,  Pathik Soni ,  Sergey Tetin ,  Lyle Yarnell

IPC: G01N33/487 ,  G01N33/543 ,  G01N33/53 ,  B01L3/00

Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.

公开(公告)号：US20180095067A1

公开(公告)日：2018-04-05

申请号：US15724200

申请日：2017-10-03

Applicant: Abbott Laboratories

Inventor： Jeffrey B. Huff ,  Mark A. Hayden ,  Peter J. Karabatsos ,  Andrew S. Schapals ,  Anthony S. Muerhoff ,  Felicia Bogdan ,  Thomas Leary ,  Shelley R. Holets-McCormack ,  Sophie Laurenson ,  Andrew T. Fischer ,  Richard Haack ,  Stefan Hershberger ,  Dustin House ,  Lei QIAO ,  M. Shawn Murphy ,  Mark R. Pope ,  Edna M. Prieto-Ballengee ,  QiaoQiao Ruan ,  Pathik Soni ,  Sergey Tetin ,  Lyle Yarnell ,  John M. Robinson

IPC: G01N33/487 ,  G01N33/53

CPC classification number: G01N33/48721 ,  G01N33/5302 ,  G01N33/54326 ,  G01N33/5438

Abstract: Integrated microfluidic and analyte detection devices are disclosed, along with methods of detecting target analytes. Digital microfluidic and analyte detection devices include a first substrate and a second substrate aligned generally parallel to each other to define a gap therebetween, the first substrate including a plurality of electrodes to generate electrical actuation forces on a liquid droplet disposed in the gap; at least one reagent disposed on at least one of the first substrate or the second substrate and configured to be carried by the liquid droplet; and an analyte detection device in fluid communication with the gap, wherein the plurality of electrodes are configured to move the liquid droplet towards the analyte detection device.

公开(公告)号：US20220276256A1

公开(公告)日：2022-09-01

申请号：US17633065

申请日：2020-08-06

Applicant: ABBOTT LABORATORIES

Inventor： Mark R. Pope ,  Stefan J. Hershberger ,  Qiaoqiao Ruan ,  Kerry M. Swift ,  Sergey Y. Tetin ,  Patrick Macdonald ,  Richard A. Haack

IPC: G01N33/58 ,  G01N33/543

Abstract: The disclosure provides kits and methods for detecting a substance that interferes with detection of an analyte in a sample and for expanding the dynamic range and reducing the hook effect of an immunoassay. The kits and methods employ two conjugates with two different detectable labels, at least one of which is a chemiluminescent compound of Formula (I).

公开(公告)号：US20180188230A1

公开(公告)日：2018-07-05

申请号：US15724213

申请日：2017-10-03

Applicant: ABBOTT LABORATORIES

Inventor： Jeffrey B. Huff ,  Mark A. Hayden ,  Peter J. Karabatsos ,  Andrew S. Schapals ,  Anthony S. Muerhoff ,  Felicia Bogdan ,  Thomas Leary ,  Shelley R. Holets-McCormack ,  Sophie Laurenson ,  Andrew T. Fischer ,  Richard Haack ,  Stefan Hershberger ,  Dustin House ,  Lei Qiao ,  M. Shawn Murphy ,  Mark R. Pope ,  Edna M. Prieto-Ballengee ,  QiaoQiao Ruan ,  Pathik Soni ,  Sergey Tetin ,  Lyle Yarnell

IPC: G01N33/487 ,  G01N33/543 ,  B01L3/00

CPC classification number: G01N33/48721 ,  B01L3/502761 ,  B01L2300/0645 ,  B01L2400/0406 ,  G01N33/5308 ,  G01N33/54306 ,  G01N2458/10

Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.