公开(公告)号：US20150268235A1

公开(公告)日：2015-09-24

申请号：US14731740

申请日：2015-06-05

Applicant: Advanced Liquid Logic, Inc.

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Ramakrishna Sista ,  Arjun Sudarsan

IPC: G01N33/543

CPC classification number: G01N33/54333 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  B03C1/02 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C2201/18 ,  B03C2201/26 ,  C12N15/1013 ,  G01N33/561 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.

Abstract translation: 本发明涉及在液滴致动器上的珠孵化和洗涤。 提供了用于将用一抗标记的磁响应珠，样品（即分析物）和二次报告基因抗体孵育在磁体上，磁铁上和从磁体上离开并完全离开磁体的方法。 还提供了使用形状辅助的液滴合并来洗涤磁响应珠的方法。 还提供了用于形状介导的分离，输送和分配含有磁响应珠的样品液滴的方法。 本发明的装置和方法提供了免疫测定中分析物的快速结果和最佳检测的时间。

公开(公告)号：US10585090B2

公开(公告)日：2020-03-10

申请号：US16132175

申请日：2018-09-14

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vamsee K. Pamula ,  Michael G. Pollack ,  Ramakrishna Sista ,  Arjun Sudarsan

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N35/00 ,  G01N1/00 ,  G01N1/10 ,  B01L3/00 ,  G01N33/543 ,  B01F13/00 ,  B03C1/02 ,  B03C1/28 ,  B03C1/30 ,  B03C5/00 ,  C12N15/10 ,  G01N33/561 ,  B01F11/00 ,  B01L7/00 ,  G01N35/10

Abstract: Methods are provided for separating magnetically responsive beads from a droplet in a droplet actuator. Droplet operations electrodes and a magnet are arranged in a droplet actuator to manipulate a bead-containing droplet and position it relative to a magnetic field region that attracts the magnetically responsive beads. The droplet operations electrodes are operated to control the droplet shape and transport it away from the magnetic field region to form a concentration of beads in the droplet. The continued transport of the droplet away from the magnetic field causes the concentration of beads to break away from the droplet to yield a small, concentrated bead-containing droplet immobilized by the magnet.

公开(公告)号：US09630180B2

公开(公告)日：2017-04-25

申请号：US14590470

申请日：2015-01-06

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Vijay Srinivasan ,  Michael G. Pollack ,  Vamsee K. Pamula ,  Zhishan Hua ,  Arjun Sudarsan ,  Philip Y. Paik

IPC: B01L3/00 ,  B01F13/00 ,  F04B19/00 ,  B01L3/02

CPC classification number: B01L3/52 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/0241 ,  B01L3/502792 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/143 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/0427 ,  B01L2400/06 ,  B01L2400/0688 ,  F04B19/006

Abstract: A droplet actuator with a droplet formation electrode configuration associated with a droplet operations surface, wherein the electrode configuration may include one or more electrodes configured to control volume of a droplet during formation of a sub-droplet on the droplet operations surface. Methods of making and using the droplet actuator are also provided.

公开(公告)号：US09358551B2

公开(公告)日：2016-06-07

申请号：US14928096

申请日：2015-10-30

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Prasanna Thwar ,  Vamsee K. Pamula ,  Arjun Sudarsan ,  Ramakrishna Sista

IPC: G01N1/10 ,  B01L3/00 ,  B03C5/00 ,  G01N33/543 ,  B03C1/01 ,  B03C1/28 ,  B03C5/02

CPC classification number: B03C7/026 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0887 ,  B01L2400/0424 ,  B01L2400/0427 ,  B03C1/01 ,  B03C1/288 ,  B03C5/00 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N33/54326

Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.

公开(公告)号：US09081007B2

公开(公告)日：2015-07-14

申请号：US14466193

申请日：2014-08-22

Applicant: Advanced Liquid Logic, Inc.

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Ramakrishna Sista ,  Arjun Sudarsan

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N33/561 ,  B01F11/00 ,  B01F13/00 ,  B01L3/00 ,  B03C1/02 ,  B03C1/30 ,  B03C5/00 ,  B01L7/00 ,  G01N35/10

CPC classification number: G01N33/54333 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  B03C1/02 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C2201/18 ,  B03C2201/26 ,  C12N15/1013 ,  G01N33/561 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The present invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.

Abstract translation: 本发明涉及在液滴致动器上的珠孵化和洗涤。 提供了用于将用一抗标记的磁响应珠，样品（即分析物）和二次报告基因抗体孵育在磁体上，磁铁上和从磁体上离开并完全离开磁体的方法。 还提供了使用形状辅助的液滴合并来洗涤磁响应珠的方法。 还提供了用于形状介导的分离，输送和分配含有磁响应珠的样品液滴的方法。 本发明的装置和方法提供了免疫测定中分析物的快速结果和最佳检测的时间。

公开(公告)号：US20150107995A1

公开(公告)日：2015-04-23

申请号：US14589314

申请日：2015-01-05

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Ramakrishna Sista ,  Vamsee Pamula ,  Arjun Sudarsan ,  Vijay Srinivasan ,  Prasanna Thwar

IPC: B01L3/00 ,  G01N27/447

CPC classification number: B01L3/502792 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2300/0819 ,  B01L2300/0851 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2400/0427 ,  B01L2400/043

Abstract: The invention provides droplet actuators and droplet actuator techniques. Among other things, the droplet actuators and methods are useful for manipulating beads on a droplet actuator, such as conducting droplet operations using bead-containing droplets on a droplet actuator. For example, beads may be manipulated on a droplet actuator in the context of executing a sample preparation protocol and/or an assay protocol. An output of the methods of the invention may be beads prepared for execution of an assay protocol. Another output of the methods of the invention may be results of an assay protocol executed using beads. Among the methods described herein are methods of concentrating beads in droplets, methods of washing beads, methods of suspending beads, methods of separating beads, methods of localizing beads within a droplet, methods of forming emulsions in which droplets include beads, methods of loading beads into a droplet operations gap of a droplet actuator, methods of organizing beads in a monolayer, and methods of capturing, trapping or restraining beads.

Abstract translation: 本发明提供了液滴致动器和液滴致动器技术。 除其他之外，液滴致动器和方法可用于操纵液滴致动器上的珠，例如使用液滴致动器上的含珠液滴进行液滴操作。 例如，在执行样品制备方案和/或测定方案的上下文中，珠可以在液滴致动器上操作。 本发明方法的输出可以是准备用于执行测定方案的珠粒。 本发明方法的另一个输出可以是使用珠子执行的测定方案的结果。 在本文描述的方法中，将珠粒集中在液滴中的方法，洗涤珠粒的方法，悬浮珠粒的方法，分离珠粒的方法，在液滴内定位珠粒的方法，形成液滴包括珠子的乳液的方法，加载珠粒的方法 液滴致动器的液滴操作间隙，单层组织珠粒的方法，以及捕获，捕获或抑制珠粒的方法。

公开(公告)号：US20140008222A1

公开(公告)日：2014-01-09

申请号：US14027960

申请日：2013-09-16

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vijay Srinivasan ,  Michael G. Pollack ,  Zhishan Hua ,  Arjun Sudarsan ,  Allen E. Eckhardt

IPC: B01L3/02

CPC classification number: G01N27/44743 ,  B01L3/0268 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/0605 ,  B01L2200/061 ,  B01L2200/0647 ,  B01L2300/0645 ,  B01L2300/16 ,  B01L2400/0415 ,  B01L2400/0427

Abstract: A method of loading a droplet actuator is provided. In one embodiment, the method may include, providing: a droplet actuator loaded with a filler fluid; a reservoir comprising a droplet fluid; and a fluid path extending from the reservoir into the droplet actuator; and forcing filler fluid from one locus in the droplet actuator to another locus in the droplet actuator; or out of the droplet actuator; thereby causing droplet fluid to flow through the fluid path and into the droplet actuator.

Abstract translation: 提供了一种装载液滴致动器的方法。 在一个实施例中，该方法可以包括：提供：负载有填充流体的液滴致动器; 包括液滴流体的储存器; 以及从储存器延伸到液滴致动器中的流体路径; 并且迫使填充液从液滴致动器中的一个轨迹到液滴致动器中的另一个轨迹; 或离开液滴致动器; 从而使液滴流体流过流体路径并进入液滴致动器。

公开(公告)号：US20200209231A1

公开(公告)日：2020-07-02

申请号：US16811789

申请日：2020-03-06

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vamsee K. Pamula ,  Michael G. Pollack ,  Ramakrishna Sista ,  Arjun Sudarsan

IPC: G01N33/543 ,  B01F11/00 ,  G01N33/561 ,  C12N15/10 ,  B03C5/00 ,  B03C1/30 ,  B03C1/28 ,  B03C1/02 ,  B01L3/00 ,  B01F13/00

Abstract: Methods are provided for separating magnetically responsive beads from a droplet in a droplet actuator. Droplet operations electrodes and a magnet are arranged in a droplet actuator to manipulate a bead-containing droplet and position it relative to a magnetic field region that attracts the magnetically responsive beads. The droplet operations electrodes are operated to control the droplet shape and transport it away from the magnetic field region to form a concentration of beads in the droplet. The continued transport of the droplet away from the magnetic field causes the concentration of beads to break away from the droplet to yield a small, concentrated bead-containing droplet immobilized by the magnet.

公开(公告)号：US10078078B2

公开(公告)日：2018-09-18

申请号：US15210634

申请日：2016-07-14

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vamsee K. Pamula ,  Michael G. Pollack ,  Ramakrishna Sista ,  Arjun Sudarsan

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N33/543 ,  B01F11/00 ,  B01F13/00 ,  B01L3/00 ,  B03C1/02 ,  B03C1/30 ,  B03C5/00 ,  G01N33/561 ,  B03C1/28 ,  C12N15/10 ,  B01L7/00 ,  G01N35/10

CPC classification number: G01N33/54333 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  B03C1/02 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C2201/18 ,  B03C2201/26 ,  C12N15/1013 ,  G01N33/561 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.

公开(公告)号：US09511369B2

公开(公告)日：2016-12-06

申请号：US14248884

申请日：2014-04-09

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vijay Srinivasan ,  Michael G. Pollack ,  Alexander Shenderov ,  Zhishan Hua ,  Arjun Sudarsan

IPC: B01L3/00

CPC classification number: B01L3/502792 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0427 ,  Y10T29/49826

Abstract: The invention provides a droplet actuator. The droplet actuator may include a base substrate and a top substrate separated to form a gap. The base substrate may include electrodes configured for conducting droplet operations in the gap; and the top substrate may include a glass substrate portion coupled to a non-glass portion, where the non-glass portion may include one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap. The invention also provides related methods of manufacturing the droplet actuator, methods of using the droplet actuator, and methods of loading the droplet actuator.

Abstract translation: 本发明提供一种液滴致动器。 液滴致动器可以包括分离以形成间隙的基底基板和顶基板。 基底基板可以包括被配置用于在间隙中进行液滴操作的电极; 并且顶部衬底可以包括联接到非玻璃部分的玻璃衬底部分，其中非玻璃部分可以包括建立从液滴致动器的外部延伸到间隙中的流体路径的一个或多个开口。 本发明还提供了制造液滴致动器的相关方法，使用液滴致动器的方法以及装载液滴致动器的方法。