公开(公告)号：US11332706B2

公开(公告)日：2022-05-17

申请号：US16774991

申请日：2020-01-28

Applicant: Calysta, Inc.

Inventor： Luan Thanh Nguyen ,  Arild Johannessen ,  Graham Ian Aylen ,  Joshua A. Silverman

IPC: C12M1/00 ,  C12N1/30 ,  C12M1/42 ,  C12M1/34

Abstract: Reactors, systems and processes for the production of biomass by culturing microorganisms in aqueous liquid culture medium circulating inner loop reactor which utilize nonvertical pressure reduction zones are described. Recovery and processing of the culture microorganisms to obtain products, such as proteins or hydrocarbons is described.

公开(公告)号：US11034930B2

公开(公告)日：2021-06-15

申请号：US16862026

申请日：2020-04-29

Applicant: Calysta, Inc.

Inventor： Luan Thanh Nguyen ,  Joshua A. Silverman ,  Graham Ian Aylen

IPC: C12M1/00 ,  C12M1/107 ,  B01D19/00 ,  C12M1/12

Abstract: Reactors, systems and processes for the production of biomass by culturing microorganisms in aqueous liquid culture medium circulating inner loop reactor which utilize nonvertical pressure reduction zones are described. Recovery and processing of the culture microorganisms to obtain products, such as proteins or hydrocarbons is described.

公开(公告)号：US09657317B2

公开(公告)日：2017-05-23

申请号：US14344899

申请日：2012-10-17

Applicant: Calysta, Inc.

Inventor： Joshua A. Silverman ,  Tom Purcell ,  Jon Edward Ness ,  Effendi Leonard

IPC: C12P7/42 ,  C12P19/32 ,  C12P7/40 ,  C12N15/52

CPC classification number: C12P7/42 ,  C12N15/52 ,  C12P7/40 ,  C12P19/32

Abstract: Provided herein is an alkane-metabolizing cell that is unable to convert propionyl-CoA into methylmalonyl-CoA or 2-metylcitrate synthase. Depending on which enzymes are present in the cell, the cell can produce acrylate or a precursor for the same (e.g., propionate, 3-hydroxypropionyl-CoA, 3-hydroxypropionate, acrylyl-CoA) that can be readily converted to acrylate enzymatically (e.g., in the cell) or by chemical treatment. In one embodiment, the cell may contain a cytochrome P450 or alkane oxidase enzyme that allows the production of 3-hydroxypropionyl-CoA, which can be readily converted to 3-hydroxypropionate. In order to make such compounds, the cell may be grown in the presence of an odd-numbered chain alkane (e.g., pentane or heptane), although another odd-numbered chain alkane may be used. In another embodiment, the cell may contain acyl-CoA oxidase, enoyl-CoA hydratase, and hydrolase.

公开(公告)号：US20160289782A1

公开(公告)日：2016-10-06

申请号：US15187509

申请日：2016-06-20

Applicant: Calysta, Inc.

Inventor： Joshua A. Silverman ,  Sol M. Resnick ,  Michael Mendez ,  Renee M. Saville ,  Sungwon Lee ,  Luan Nguyen

IPC: C12R1/01 ,  C12N9/16 ,  C12P7/64

CPC classification number: C12P7/6409 ,  C10G3/00 ,  C10G3/50 ,  C10G47/00 ,  C10G2300/1014 ,  C10L1/02 ,  C10L1/04 ,  C10L2200/0469 ,  C10L2270/02 ,  C10L2270/04 ,  C10L2290/26 ,  C12N1/16 ,  C12N1/20 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/93 ,  C12N15/52 ,  C12N15/74 ,  C12P5/00 ,  C12P7/6463 ,  C12P7/649 ,  C12R1/01 ,  C12Y203/01039 ,  C12Y301/02 ,  C12Y602/01003 ,  C12Y604/01002 ,  Y02E50/13 ,  Y02E50/343 ,  Y02P20/52 ,  Y02P30/20

Abstract: The present disclosure relates to bioengineering approaches for producing biofuel and, in particular, to the use of a C1 metabolizing microorganism reactor system for converting C1 substrates, such as methane or methanol, into biomass and subsequently into biofuels, bioplastics, or the like.

公开(公告)号：US20160289714A1

公开(公告)日：2016-10-06

申请号：US15187492

申请日：2016-06-20

Applicant: Calysta, Inc.

Inventor： Joshua A. Silverman ,  Sol M. Resnick ,  Michael Mendez ,  Renee M. Saville ,  Sungwon Lee ,  Luan Nguyen

IPC: C12P7/64 ,  C12N15/74 ,  C12N9/00 ,  C12N15/52 ,  C12N9/16 ,  C12N9/10

CPC classification number: C12P7/6409 ,  C10G3/00 ,  C10G3/50 ,  C10G47/00 ,  C10G2300/1014 ,  C10L1/02 ,  C10L1/04 ,  C10L2200/0469 ,  C10L2270/02 ,  C10L2270/04 ,  C10L2290/26 ,  C12N1/16 ,  C12N1/20 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/93 ,  C12N15/52 ,  C12N15/74 ,  C12P5/00 ,  C12P7/6463 ,  C12P7/649 ,  C12R1/01 ,  C12Y203/01039 ,  C12Y301/02 ,  C12Y602/01003 ,  C12Y604/01002 ,  Y02E50/13 ,  Y02E50/343 ,  Y02P20/52 ,  Y02P30/20

Abstract: The present disclosure relates to bioengineering approaches for producing biofuel and, in particular, to the use of a C1 metabolizing microorganism reactor system for converting C1 substrates, such as methane or methanol, into biomass and subsequently into biofuels, bioplastics, or the like.

公开(公告)号：US20150197779A1

公开(公告)日：2015-07-16

申请号：US14599383

申请日：2015-01-16

Applicant: CALYSTA, INC.

Inventor： Renee M. Saville ,  Joshua A. Silverman ,  Eric G. Luning ,  Brandon D. Doss ,  Lorraine Joan Giver ,  Sol M. Resnick ,  Drew D. Regitsky

IPC: C12P13/08

CPC classification number: C12P13/08 ,  C12N1/30 ,  C12N15/52 ,  C12P13/12

Abstract: The present disclosure relates to recombinant microorganisms engineered for enhance production of a desired amino acid, as well as related biomass, and compositions which are useful, inter alia, as animal feed ingredients. The present invention also provides related methods.

Abstract translation: 本公开涉及用于增强所需氨基酸生产的重组微生物以及相关的生物质，以及特别可用作动物饲料成分的组合物。 本发明还提供了相关方法。

公开(公告)号：US11795428B2

公开(公告)日：2023-10-24

申请号：US16477139

申请日：2018-01-09

Applicant: Calysta, Inc.

Inventor： Luan Thanh Nguyen ,  Joshua A. Silverman

IPC: C12M3/00 ,  C12M1/00 ,  C12M1/04 ,  C12M1/34 ,  C12N1/30

CPC classification number: C12M29/20 ,  C12M27/04 ,  C12M29/14 ,  C12M29/18 ,  C12M29/26 ,  C12M41/34 ,  C12M41/40 ,  C12N1/30

Abstract: Reactors, systems and processes for the production of biomass by culturing microorganisms in aqueous liquid culture medium circulating in a loop reactor which utilize substantially vertical flow zones are described. Recovery and processing of the culture microorganisms to obtain products, such as proteins or hydrocarbons is described.

公开(公告)号：US20220259548A1

公开(公告)日：2022-08-18

申请号：US17732263

申请日：2022-04-28

Applicant: Calysta, Inc.

Inventor： Luan Thanh NGUYEN ,  Arild Johannessen ,  Graham Ian Aylen ,  Joshua A. Silverman

IPC: C12M1/00 ,  C12M1/42 ,  C12M1/34 ,  C12N1/30

Abstract: Reactors, systems and processes for the production of biomass by culturing microorganisms in aqueous liquid culture medium circulating inner loop reactor which utilize nonvertical pressure reduction zones are described. Recovery and processing of the culture microorganisms to obtain products, such as proteins or hydrocarbons is described.

公开(公告)号：US10689610B2

公开(公告)日：2020-06-23

申请号：US16102448

申请日：2018-08-13

Applicant: Calysta, Inc.

Inventor： Luan Thanh Nguyen ,  Joshua A. Silverman ,  Graham Ian Aylen

IPC: C12M1/00 ,  C12M1/107 ,  B01D19/00 ,  C12M1/12

Abstract: Reactors, systems and processes for the production of biomass by culturing microorganisms in aqueous liquid culture medium circulating inner loop reactor which utilize nonvertical pressure reduction zones are described. Recovery and processing of the culture microorganisms to obtain products, such as proteins or hydrocarbons is described.

公开(公告)号：US10570364B2

公开(公告)日：2020-02-25

申请号：US15625858

申请日：2017-06-16

Applicant: Calysta, Inc.

Inventor： Luan Thanh Nguyen ,  Arild Johannessen ,  Graham Ian Aylen ,  Joshua A. Silverman

IPC: C12N1/30 ,  C12M1/00 ,  C12M1/42 ,  C12M1/34

Abstract: Reactors, systems and processes for the production of biomass by culturing microorganisms in aqueous liquid culture medium circulating inner loop reactor which utilize nonvertical pressure reduction zones are described. Recovery and processing of the culture microorganisms to obtain products, such as proteins or hydrocarbons is described.