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    Host cells and method for making acrylate and precursors thereof using an odd-numbered alkane feedstock
    1.
    发明授权
    Host cells and method for making acrylate and precursors thereof using an odd-numbered alkane feedstock 有权

    公开(公告)号：US09657317B2

    公开(公告)日：2017-05-23

    申请号：US14344899

    申请日：2012-10-17

    Applicant: Calysta, Inc.

    Inventor： Joshua A. Silverman ,  Tom Purcell ,  Jon Edward Ness ,  Effendi Leonard

    IPC: C12P7/42 ,  C12P19/32 ,  C12P7/40 ,  C12N15/52

    CPC classification number: C12P7/42 ,  C12N15/52 ,  C12P7/40 ,  C12P19/32

    Abstract: Provided herein is an alkane-metabolizing cell that is unable to convert propionyl-CoA into methylmalonyl-CoA or 2-metylcitrate synthase. Depending on which enzymes are present in the cell, the cell can produce acrylate or a precursor for the same (e.g., propionate, 3-hydroxypropionyl-CoA, 3-hydroxypropionate, acrylyl-CoA) that can be readily converted to acrylate enzymatically (e.g., in the cell) or by chemical treatment. In one embodiment, the cell may contain a cytochrome P450 or alkane oxidase enzyme that allows the production of 3-hydroxypropionyl-CoA, which can be readily converted to 3-hydroxypropionate. In order to make such compounds, the cell may be grown in the presence of an odd-numbered chain alkane (e.g., pentane or heptane), although another odd-numbered chain alkane may be used. In another embodiment, the cell may contain acyl-CoA oxidase, enoyl-CoA hydratase, and hydrolase.

    Nucleic acids and vectors for use with methanotrophic bacteria
    7.
    发明授权
    Nucleic acids and vectors for use with methanotrophic bacteria 有权

    公开(公告)号：US10443060B2

    公开(公告)日：2019-10-15

    申请号：US15319312

    申请日：2015-06-18

    Applicant: Calysta, Inc.

    Inventor： Renee M. Saville ,  Joshua Silverman ,  Jeremy Minshull ,  Jon Edward Ness ,  Effendi Leonard ,  Jana Stumpe ,  Mark Welch

    IPC: C12N15/74 ,  C12N9/04

    Abstract: The present disclosure provides nucleic acids and vectors for use with methanotrophic bacteria. Related host cells and methods for using such nucleic acids and vectors for expressing polypeptides or other genetic manipulation of methanotrophic bacteria are also provided. In one aspect, the present disclosure is directed to a non-naturally occurring nucleic acid molecule, comprising (1) a promoter that is functional in a methanotrophic bacterium, and (2) a native or altered methanol dehydrogenase (MDH) ribosomal binding sequence, provided that when the promoter is an MDH gene promoter, the nucleic acid comprises an altered MDH ribosomal binding sequence.

    Host Cells and Method for Making Acrylate and Precursors Thereof Using an Odd-Numbered Alkane Feedstock
    8.
    发明申请
    Host Cells and Method for Making Acrylate and Precursors Thereof Using an Odd-Numbered Alkane Feedstock 有权
    使用奇数烷烃原料制备丙烯酸酯及其前体的宿主细胞和方法

    公开(公告)号：US20150087036A1

    公开(公告)日：2015-03-26

    申请号：US14344899

    申请日：2012-10-17

    Applicant: Calysta, Inc.

    Inventor： Joshua A. Silverman ,  Tom Purcell ,  Jon Edward Ness ,  Effendi Leonard

    IPC: C12P7/42 ,  C12P7/40

    CPC classification number: C12P7/42 ,  C12N15/52 ,  C12P7/40 ,  C12P19/32

    Abstract: Provided herein is an alkane-metabolizing cell that is unable to convert propionyl-CoA into methylmalonyl-CoA or 2-metylcitrate synthase. Depending on which enzymes are present in the cell, the cell can produce acrylate or a precursor for the same (e.g., propionate, 3-hydroxypropionyl-CoA, 3-hydroxypropionate, acrylyl-CoA) that can be readily converted to acrylate enzymatically (e.g., in the cell) or by chemical treatment. In one embodiment, the cell may contain a cytochrome P450 or alkane oxidase enzyme that allows the production of 3-hydroxypropionyl-CoA, which can be readily converted to 3-hydroxypropionate. In order to make such compounds, the cell may be grown in the presence of an odd-numbered chain alkane (e.g., pentane or heptane), although another odd-numbered chain alkane may be used. In another embodiment, the cell may contain acyl-CoA oxidase, enoyl-CoA hydratase, and hydrolase.

    Abstract translation: 本文提供的是不能将丙酰辅酶A转化为甲基丙二酰辅酶A或2-甲基柠檬酸合酶的烷烃代谢细胞。 根据细胞中哪些酶的存在，细胞可以生成丙烯酸酯或相同的前体（例如，丙酸酯，3-羟基丙酰基-CoA，3-羟基丙酸酯，丙烯酰辅酶A），其可以容易地酶转化成丙烯酸酯（例如 ，在细胞中）或通过化学处理。 在一个实施方案中，细胞可以含有细胞色素P450或烷烃氧化酶，其允许产生3-羟基丙酰辅酶A，其可以容易地转化为3-羟基丙酸酯。 为了制备这种化合物，可以在奇数链烷烃（例如戊烷或庚烷）的存在下生长电池，尽管可以使用另一个奇数链烷烃。 在另一个实施方案中，细胞可以含有酰基辅酶A氧化酶，烯酰辅酶A水合酶和水解酶。

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