公开(公告)号：US09816122B2

公开(公告)日：2017-11-14

申请号：US14430930

申请日：2013-09-25

Applicant: Glycom A/S

Inventor： Pauline Peltier-Pain ,  Gyula Dekany ,  Christophe Pain ,  Pierre Chassagne ,  Nicolas Fierfort ,  Dóra Molnàr-Gàbor

IPC: C12P19/18 ,  C12P19/44 ,  C07H15/08 ,  C07H13/04 ,  C07H15/04 ,  C07H15/26

CPC classification number: C12P19/44 ,  C07H13/04 ,  C07H15/04 ,  C07H15/08 ,  C07H15/26 ,  C12P19/18

Abstract: The invention relates to a method for producing a glycoconjugate comprising an oligosaccharide part covalently linked to a non-sugar moiety selected from the group consisting of amino acids, peptides, proteins, lipids, longer alkyl groups, polyethylene glycols, α,β-unsaturated amido group and polyvinyl alcohols, using a genetically modified cell.

公开(公告)号：US20150252400A1

公开(公告)日：2015-09-10

申请号：US14430930

申请日：2013-09-25

Applicant: GLYCOM A/S

Inventor： Pauline Peltier-Pain ,  Gyula Dekany ,  Christophe Pain ,  Pierre Chassagne ,  Nicolas Fierfort ,  Dóra Molnàr-Gàbor

IPC: C12P19/44 ,  C07H15/08 ,  C07H13/04

CPC classification number: C12P19/44 ,  C07H13/04 ,  C07H15/04 ,  C07H15/08 ,  C07H15/26 ,  C12P19/18

Abstract: The invention relates to a method for producing a glycoconjugate comprising an oligosaccharide part covalently linked to a non-sugar moiety selected from the group consisting of amino acids, peptides, proteins, lipids, longer alkyl groups, polyethylene glycols, α,β-unsaturated amido group and polyvinyl alcohols, using a genetically modified cell.

Abstract translation: 本发明涉及一种制备糖缀合物的方法，其包含与非糖部分共价连接的寡糖部分，所述寡糖部分选自氨基酸，肽，蛋白质，脂质，较长的烷基，聚乙二醇，α，β-不饱和 酰胺基和聚乙烯醇，使用转基因细胞。

公开(公告)号：US12060594B2

公开(公告)日：2024-08-13

申请号：US16895871

申请日：2020-06-08

Applicant: Glycom A/S

Inventor： Gyula Dekany ,  Györgyi Osztrovszky ,  Martin Matwiejuk ,  Pierre Chassagne ,  Markus Jondelius Hederos

IPC: C12P19/18 ,  C07H1/06 ,  C07H3/06

CPC classification number: C12P19/18 ,  C07H1/06 ,  C07H3/06 ,  C12Y204/01069

Abstract: The invention relates to a method for selective crystallization of 2′-fucosyllactose (2′-FL) from an aqueous solution comprising 2′-FL and one or more other fucosylated carbohydrates by adding acetic acid to the solution. The aqueous solution is separated from an aqueous culture medium prior to crystallization.

公开(公告)号：US11384110B2

公开(公告)日：2022-07-12

申请号：US16346057

申请日：2017-10-31

Applicant: Glycom A/S

Inventor： Pierre Chassagne ,  Martin Matwiejuk ,  Nikolay Khanzhin ,  Markus Jondelius Hederos

IPC: C07H3/06 ,  A61K31/702 ,  F26B5/04 ,  F26B21/14 ,  C07H13/04 ,  C07H1/00 ,  A23L33/21 ,  C07H1/06

Abstract: The present invention relates to a method of removing residual organic solvent from crystalline oligosaccharides by the use of water vapour as well as the pharmaceutically and nutritionally suitable crystalline oligosaccharides obtained by said method.

公开(公告)号：US11312741B2

公开(公告)日：2022-04-26

申请号：US16094508

申请日：2017-04-19

Applicant: GLYCOM A/S

Inventor： Pierre Chassagne ,  Nikolay Khanzhin ,  Martin Matwiejuk ,  Markus Jondelius Hederos ,  Niels Banke

IPC: C07H13/04 ,  B01D61/02 ,  B01D61/14 ,  B01D61/58 ,  C07H1/06 ,  C12P19/28 ,  B01D15/36 ,  B01J41/07 ,  B01D61/16 ,  B01J39/05 ,  B01D15/18 ,  B01J20/20 ,  G01N30/96 ,  C12P19/26 ,  G01N30/02

Abstract: The invention relates to a method for obtaining an N-acetylglucosamine containing neutral oligosaccharide from a fermentation broth, wherein said oligosaccharide is produced by culturing a genetically modified microorganism capable of producing said oligosaccharide from an internalized carbohydrate precursor, comprising the steps of: i) ultrafiltration (UF), preferably to separate biomass from the broth, ii) nanofiltration (NF), preferably to concentrate said oligosaccharide in the broth and/or reduce an inorganic salt content of the broth, and iii) treating the broth with an ion exchange resin, preferably to remove charged materials, and/or subjecting the broth to chromatography, preferably to remove hydrophobic impurities.

公开(公告)号：US10005807B2

公开(公告)日：2018-06-26

申请号：US14783783

申请日：2014-04-11

Applicant: GLYCOM A/S

Inventor： Pierre Chassagne ,  Nikolay Khanzhin ,  Markus Jondelius Hederos ,  Elise Champion ,  Martin Matwiejuk ,  Gyula Dekany

IPC: C07H5/04 ,  C07H1/00

CPC classification number: C07H5/04 ,  C07H1/00

Abstract: An achemo-enzymatic synthesis of oligosaccharides of formula 1 is presented wherein R is selected from —OH, —N 3 and —OR 6 wherein R 6 is selected from allyl optionally substituted by one or more methyl, propargyl optionally substituted by one or more methyl, 2-trimethylsilyl-ethyl, —(CH 2) n —NH 2 and —(CH 2) n —N 3 wherein integer n is to 10, preferably 2 or 3, R is selected from sialyl moiety, —SO 3 H and —CH(R)—COOH wherein R is selected from H, alkyl and benzyl, R 2 is selected from H and fucosyl, R 3 is selected from H and sialyl, R 4 is selected from H and fucosyl, provided that at least one of R 3 and R 4 is H, and A is a divalent carbohydrate linker, having important biological activities and significant commercial value for the pharmaceutical and food industry.