公开(公告)号：US10828679B2

公开(公告)日：2020-11-10

申请号：US16663099

申请日：2019-10-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James A. Ball ,  Phil Waggoner ,  Scott Parker

IPC: G01N27/414 ,  B08B3/08 ,  B08B9/08 ,  C11D3/16 ,  C11D3/34 ,  C11D3/43 ,  C11D1/22 ,  C11D11/00 ,  H01L21/02 ,  C11D7/50 ,  C11D7/34 ,  B05D3/00 ,  B05D5/12

Abstract: A method of treating a sensor array including a plurality of sensors and an isolation structure, where a sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array and the isolation structure is disposed between the sensor pad and sensor pads of other sensors of the plurality of sensors, comprises exposing the sensor pad and the isolation structure to a non-aqueous organo-silicon solution including an organo-silicon compound and a first non-aqueous carrier; applying an acid solution including an organic acid and a second non-aqueous carrier to the sensor pad; and rinsing the acid solution from the sensor pad and the isolation structure.

公开(公告)号：US10576396B2

公开(公告)日：2020-03-03

申请号：US15375271

申请日：2016-12-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Brian Reed ,  James A. Ball

IPC: C12Q1/6806 ,  B01D17/04 ,  C07H1/06 ,  C07H21/00

Abstract: A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.

公开(公告)号：US11879156B2

公开(公告)日：2024-01-23

申请号：US17818869

申请日：2022-08-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Phil Waggoner ,  James A. Ball ,  Wolfgang Hinz ,  Michael L. Minto ,  Scott Parker ,  David M. Cox ,  Alexander Mastroianni ,  Jeremy Gray ,  Marc Glazer ,  Kimberly Gorrell

IPC: G01N27/414 ,  C12Q1/6869

CPC classification number: C12Q1/6869 ,  G01N27/4145 ,  C12Q1/6869 ,  C12Q2527/119 ,  C12Q2527/125 ,  C12Q2565/607

Abstract: A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

公开(公告)号：US11833450B2

公开(公告)日：2023-12-05

申请号：US17566511

申请日：2021-12-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Brian Reed ,  James A. Ball

IPC: B01D17/04 ,  C07H1/06 ,  C07H21/00 ,  C12Q1/6806

CPC classification number: B01D17/047 ,  B01D17/04 ,  B01D17/041 ,  C07H1/06 ,  C07H21/00 ,  C12Q1/6806 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2527/137 ,  C12Q2563/159

Abstract: A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.

公开(公告)号：US11319587B2

公开(公告)日：2022-05-03

申请号：US16292087

申请日：2019-03-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang Hinz ,  David Light ,  Todd Rearick ,  James A. Ball

IPC: C12Q1/6874 ,  G01N27/414

Abstract: The disclosure relates to novel particle compositions and methods of making said compositions having applications in nucleic acid analysis, as well as apparatuses and systems for the same.

公开(公告)号：US20210102916A1

公开(公告)日：2021-04-08

申请号：US17060680

申请日：2020-10-01

Applicant: Life Technologies Corporation

Inventor： Ronald L. Cicero ,  Marc Glazer ,  Yufang Wang ,  Christina E. Inman ,  Jeremy Gray ,  Joseph Koscinski ,  James A. Ball ,  Phil Waggoner ,  Alexander Mastroianni

IPC: G01N27/414

Abstract: A sensor component includes a sensor including a sensor surface and a reaction site in cooperation with the sensor and exposing the sensor surface. The reaction site including a reaction site surface. A surface agent is bound to the reaction site surface or the sensor surface. The surface agent includes a surface active functional group reactive with Bronsted base or Lewis acid functionality on the reaction site surface or the sensor surface and including distal functionality that does not have a donor electron pair.

公开(公告)号：US20200216893A1

公开(公告)日：2020-07-09

申请号：US16824920

申请日：2020-03-20

Applicant: Life Technologies Corporation

Inventor： Phil Waggoner ,  James A. Ball ,  Wolfgang Hinz ,  Michael L. Minto ,  Scott Parker ,  David M. Cox ,  Alexander Mastroianni ,  Jeremy Gray ,  Marc Glazer ,  Kimberly Gorrell

IPC: C12Q1/6869 ,  G01N27/414

Abstract: A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

公开(公告)号：US10456814B2

公开(公告)日：2019-10-29

申请号：US16182429

申请日：2018-11-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James A. Ball ,  Phil Waggoner ,  Scott Parker

IPC: B08B9/08 ,  B08B3/08 ,  C11D3/16 ,  G01N27/414 ,  C11D3/34 ,  C11D3/43 ,  C11D1/22 ,  C11D11/00 ,  H01L21/02 ,  C11D7/50 ,  C11D7/34 ,  B05D3/00 ,  B05D5/12

Abstract: A method of treating a sensor array including a plurality of sensors and an isolation structure, where a sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array and the isolation structure is disposed between the sensor pad and sensor pads of other sensors of the plurality of sensors, comprises exposing the sensor pad and the isolation structure to a non-aqueous organo-silicon solution including an organo-silicon compound and a first non-aqueous carrier; applying an acid solution including an organic acid and a second non-aqueous carrier to the sensor pad; and rinsing the acid solution from the sensor pad and the isolation structure.

公开(公告)号：US10107825B2

公开(公告)日：2018-10-23

申请号：US14741084

申请日：2015-06-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James A. Ball ,  Jonathan Schultz

IPC: C12Q1/68 ,  G01N33/84 ,  C12Q1/6869 ,  B01J19/00

Abstract: A buffered suspension includes a surfactant and a solid buffer particulate having a point of zero charge at least 1.2 pH units different that the pH of the buffered suspension. The buffered suspension can be prepared by mixing a stock solution with the solid buffer particulate and titrating. A method of preforming a pH sensitive process includes drawing the buffered suspension from a reservoir, filtering the solid buffer particulate from the buffered suspension, and applying the filtered solution to a sensor.

公开(公告)号：US09890424B2

公开(公告)日：2018-02-13

申请号：US14742404

申请日：2015-06-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan Schultz ,  Todd Roswech ,  Jon A. Hoshizaki ,  Albert L. Carrillo ,  James A. Ball

IPC: C12Q1/68 ,  G01N35/10 ,  G01N1/00 ,  B01F5/06 ,  B01F11/00 ,  B01F1/00 ,  B01L3/00 ,  G01N27/414

CPC classification number: C12Q1/6869 ,  B01F1/0027 ,  B01F5/0683 ,  B01F11/0048 ,  B01F11/0071 ,  B01L3/502 ,  B01L3/505 ,  B01L3/527 ,  B01L3/563 ,  B01L2200/04 ,  B01L2200/16 ,  B01L2300/022 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  B01L2400/0683 ,  C12Q1/6806 ,  G01N27/4145 ,  G01N35/1002 ,  G01N35/1095 ,  Y10T436/11 ,  Y10T436/143333

Abstract: A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.