公开(公告)号：US12226745B2

公开(公告)日：2025-02-18

申请号：US16403339

申请日：2019-05-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Abraham Rosenbaum ,  Collyn Seeger ,  Jeremy Gray ,  Hua Yu

IPC: C12Q1/6853 ,  B01J19/00 ,  C12Q1/6844 ,  C40B50/14

Abstract: The present disclosure provides methods, compositions and kits as well as systems for manipulating nucleic acids, including implementing isothermal amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using a pre-seeded solid support. Provided are rapid and efficient methods for generating template nucleic acid molecules comprising specific nucleotide sequence bound to solid support. Such methods can be used, for example, in manipulating nucleic acids in preparation for analysis methods that utilize monoclonal populations of nucleic acids.

公开(公告)号：US20230037531A1

公开(公告)日：2023-02-09

申请号：US17818869

申请日：2022-08-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Phil Waggoner ,  James A. Ball ,  Wolfgang Hinz ,  Michael L. Minto ,  Scott Parker ,  David M. Cox ,  Alexander Mastroianni ,  Jeremy Gray ,  Marc Glazer ,  Kimberly Gorrell

IPC: C12Q1/6869 ,  G01N27/414

Abstract: A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

公开(公告)号：US11293056B2

公开(公告)日：2022-04-05

申请号：US16183722

申请日：2018-11-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Abraham Rosenbaum ,  Collyn Seeger ,  Jeremy Gray ,  Hua Yu

IPC: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6853 ,  C12Q1/6876 ,  C12Q1/6874

Abstract: The present disclosure provides methods, compositions and kits as well as systems for manipulating nucleic acids, including implementing isothermal amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using a pre-seeded solid support. Provided are rapid and efficient methods for generating template nucleic acid molecules comprising specific nucleotide sequence bound to solid support. Such methods can be used, for example, in manipulating nucleic acids in preparation for analysis methods that utilize monoclonal populations of nucleic acids.

公开(公告)号：US20190255505A1

公开(公告)日：2019-08-22

申请号：US16403339

申请日：2019-05-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Abraham Rosenbaum ,  Collyn Seeger ,  Jeremy Gray ,  Hua Yu

IPC: B01J19/00 ,  C12Q1/6853

Abstract: The present disclosure provides methods, compositions and kits as well as systems for manipulating nucleic acids, including implementing isothermal amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using a pre-seeded solid support. Provided are rapid and efficient methods for generating template nucleic acid molecules comprising specific nucleotide sequence bound to solid support. Such methods can be used, for example, in manipulating nucleic acids in preparation for analysis methods that utilize monoclonal populations of nucleic acids.

公开(公告)号：US20250161901A1

公开(公告)日：2025-05-22

申请号：US19029066

申请日：2025-01-17

Applicant: Life Technologies Corporation

Inventor： David Light ,  Ronald L. Cicero ,  Yufang Wang ,  Alexander Mastroianni ,  Prasanna Krishnan Thwar ,  Jeremy Gray ,  Marc Glazer ,  Tommie Lloyd Lincecum, JR. ,  Mohammad Alanjary ,  Joseph Koscinski

IPC: B01J19/00 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: A method of loading beads on a sensor substrate includes applying a suspension including beads to a flow cell defined over a sensor substrate. The sensor substrate includes a plurality of wells. The beads at least partially deposit into the plurality of wells. The method also includes removing liquid from the flow cell, evaporating liquid from the flow cell, for example, by drawing air through the flow cell; and applying a hydrating solution to the flow cell.

公开(公告)号：US20250145982A1

公开(公告)日：2025-05-08

申请号：US19020341

申请日：2025-01-14

Applicant: Life Technologies Corporation

Inventor： Hua Yu ,  Xiaoling Yang ,  Rui Zheng ,  Chiu Tai Andrew Wong ,  Jeremy Gray

IPC: C12N15/10 ,  C12Q1/6844 ,  G01N33/543

Abstract: The disclosure generally relates to systems, methods, and apparatuses for magnetic bead loading. An example embodiment of the disclosure relates to an apparatus including a vertically oriented plate having a first major surface and a second major surface opposite the first major surface; a magnet holder securing a magnet in proximity to the first major surface of the vertically oriented plate; a drive mechanism coupled to the magnet holder and operable to move the magnet holder and magnet in parallel to the first major surface of the vertically oriented plate; and a substrate holder to receive a substrate and hold the substrate in a vertical orientation against the surface of the vertically oriented plate.

公开(公告)号：US11782019B2

公开(公告)日：2023-10-10

申请号：US17060680

申请日：2020-10-01

Applicant: Life Technologies Corporation

Inventor： Ronald L. Cicero ,  Marc Glazer ,  Yufang Wang ,  Christina E Inman ,  Jeremy Gray ,  Joseph Koscinski ,  James A. Ball ,  Phil Waggoner ,  Alexander Mastroianni

IPC: G01N27/414

CPC classification number: G01N27/4145

Abstract: A sensor component includes a sensor including a sensor surface and a reaction site in cooperation with the sensor and exposing the sensor surface. The reaction site including a reaction site surface. A surface agent is bound to the reaction site surface or the sensor surface. The surface agent includes a surface active functional group reactive with Bronsted base or Lewis acid functionality on the reaction site surface or the sensor surface and including distal functionality that does not have a donor electron pair.