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    Polymerase compositions, methods of making and using same
    12.
    发明授权
    Polymerase compositions, methods of making and using same 有权

    公开(公告)号：US09976128B2

    公开(公告)日：2018-05-22

    申请号：US14754203

    申请日：2015-06-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter Vander Horn ,  Theo Nikiforov ,  Guobin Luo ,  Mindy Landes ,  Daniel Mazur ,  Eileen Tozer ,  Tommie Lincecum

    IPC: C12N9/12 ,  C12Q1/68

    CPC classification number: C12N9/1252 ,  C12Q1/6846 ,  C12Q1/686 ,  C12Y207/07007

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

    LINKING METHODS, COMPOSITIONS, SYSTEMS, KITS AND APPARATUSES
    13.
    发明申请
    LINKING METHODS, COMPOSITIONS, SYSTEMS, KITS AND APPARATUSES 审中-公开

    公开(公告)号：US20180105805A1

    公开(公告)日：2018-04-19

    申请号：US15786223

    申请日：2017-10-17

    Applicant: Life Technologies Corporation

    Inventor： John DAVIDSON ,  Theo Nikiforov ,  Guobin Luo

    IPC: C12N11/10 ,  C12N9/12

    CPC classification number: C12N11/10 ,  C12N9/1241 ,  C12Q1/68 ,  G01N2333/9126

    Abstract: In some embodiments, the disclosure relates generally to methods as well as related compositions, systems, kits and apparatus comprising linking proteins to target compounds and/or to locations of interest using tethers. For example, the tether can be used to link the protein to a target compound, for example, to link an enzyme to a substrate. Similarly, the tether can be used to link the protein at or near a desired location on a surface. In one group of embodiments, the tether includes a polynucleotide and the target compound or location on the surface includes another polynucleotide that is capable of hybridizing to the tether. In such embodiments, the tether can be used to link the protein to the target compound or location using nucleic acid hybridization.

    Polymerase compositions, methods of making and using same
    15.
    发明授权
    Polymerase compositions, methods of making and using same 有权

    公开(公告)号：US09657281B2

    公开(公告)日：2017-05-23

    申请号：US14502201

    申请日：2014-09-30

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter Vander Horn ,  Daniel Mazur ,  Theo Nikiforov ,  Mindy Landes ,  Eileen Tozer

    IPC: C12N9/12 ,  C12Q1/68

    CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Q1/6869

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

    Labeled enzyme compositions, methods and systems
    16.
    发明授权
    Labeled enzyme compositions, methods and systems 有权
    标记的酶组合物，方法和系统

    公开(公告)号：US09567629B2

    公开(公告)日：2017-02-14

    申请号：US14284187

    申请日：2014-05-21

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Theo Nikiforov

    IPC: C12P19/34 ,  C12Q1/68 ,  C07H19/20 ,  C12N9/12 ,  C12N9/96 ,  G01N33/58 ,  G01N21/64

    CPC classification number: C12Q1/6869 ,  C07H19/20 ,  C12N9/1241 ,  C12N9/1252 ,  C12N9/96 ,  C12Q1/6818 ,  C12Y207/07 ,  C12Y207/07007 ,  G01N21/6428 ,  G01N33/582 ,  G01N2021/6432

    Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are labeled polymerase conjugates including a polymerase linked to one or more labels, wherein the conjugate has polymerase activity. Such conjugates can exhibit enhanced biological activity and/or superior detectability as compared to conventional labeled polymerases. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.

    Abstract translation: 本文公开了包含连接到具有生物活性并可用于多种生物应用的标签的生物分子的缀合物。 例如，本文提供了标记的聚合酶缀合物，包括与一个或多个标记物连接的聚合酶，其中缀合物具有聚合酶活性。 与常规标记的聚合酶相比，这种缀合物可以表现出增强的生物学活性和/或优异的可检测性。 本文还公开了制备这种缀合物的改进方法，以及在生物应用中使用这种缀合物的方法和系统，例如核苷酸掺入，引物延伸和单分子测序。

    Polymerase compositions, methods of making and using same
    18.
    发明授权
    Polymerase compositions, methods of making and using same 有权

    公开(公告)号：US11208636B2

    公开(公告)日：2021-12-28

    申请号：US16850629

    申请日：2020-04-16

    Applicant: Life Technologies Corporation

    Inventor： Peter Vander Horn ,  Theo Nikiforov ,  Guobin Luo ,  Mindy Landes ,  Daniel Mazur ,  Eileen Tozer ,  Tommie Lincecum

    IPC: C12N9/12 ,  C12Q1/6844 ,  C12Q1/686

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

    Polymerase compositions, methods of making and using same
    19.
    发明授权
    Polymerase compositions, methods of making and using same 有权

    公开(公告)号：US11091746B2

    公开(公告)日：2021-08-17

    申请号：US16894469

    申请日：2020-06-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter Vander Horn ,  Daniel Mazur ,  Theo Nikiforov ,  Mindy Landes ,  Eileen Tozer

    IPC: C12N9/12 ,  C12Q1/6869 ,  C12Q1/686

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

    Thiolated nucleotide analogues for nucleic acid synthesis
    20.
    发明授权
    Thiolated nucleotide analogues for nucleic acid synthesis 有权

    公开(公告)号：US11047004B2

    公开(公告)日：2021-06-29

    申请号：US16421284

    申请日：2019-05-23

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Steven Menchen ,  Theo Nikiforov ,  Barnett Rosenblum

    IPC: C12Q1/6869 ,  G01N27/414

    Abstract: The present disclosure provide systems, compositions, methods, reagents, kits and products for extending a nucleic acid that includes incorporating a nucleotide residue at a terminus of a nucleic acid using a polymerase enzyme and at least one nucleotide, wherein the at least one nucleotide includes a thiophosphate moiety, and wherein the at least one nucleotide is resistant to hydrolysis by phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of a phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of at least on chelation moiety that is configured to bind an orthophosphate moiety.

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