公开(公告)号：US09134269B2

公开(公告)日：2015-09-15

申请号：US13794637

申请日：2013-03-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo

IPC: G01N27/414 ,  C12Q1/68 ,  H01L23/00 ,  H01L29/78 ,  H01L21/306

CPC classification number: C12Q1/6869 ,  C12Q1/6874 ,  G01N27/414 ,  G01N27/4145 ,  G01N27/4148 ,  H01L21/306 ,  H01L24/18 ,  H01L24/20 ,  H01L24/82 ,  H01L27/088 ,  H01L29/78 ,  H01L2224/04105 ,  H01L2224/16 ,  H01L2224/76155 ,  H01L2224/82102 ,  H01L2924/01005 ,  H01L2924/01006 ,  H01L2924/01011 ,  H01L2924/01012 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01019 ,  H01L2924/0102 ,  H01L2924/01023 ,  H01L2924/01024 ,  H01L2924/01027 ,  H01L2924/01029 ,  H01L2924/0103 ,  H01L2924/01033 ,  H01L2924/01046 ,  H01L2924/01047 ,  H01L2924/01052 ,  H01L2924/01056 ,  H01L2924/01059 ,  H01L2924/01073 ,  H01L2924/01074 ,  H01L2924/01075 ,  H01L2924/01082 ,  H01L2924/1306 ,  H01L2924/13091 ,  H01L2924/14 ,  H01L2924/1433 ,  H01L2924/19041 ,  H01L2924/19042 ,  H01L2924/19043 ,  H01L2924/30105 ,  H01L2924/3011 ,  H01L2924/3025 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2533/101 ,  H01L2924/00

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

公开(公告)号：US09005427B2

公开(公告)日：2015-04-14

申请号：US14151270

申请日：2014-01-09

Applicant: Life Technologies Corporation

Inventor： Jonathan Rothberg ,  Wolfgang Hinz

IPC: G01N27/414 ,  G01N33/50 ,  G01N33/543 ,  C12Q1/68 ,  H01L29/78 ,  G01N27/26 ,  G01N33/68

CPC classification number: C12Q1/6825 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/26 ,  G01N27/4145 ,  G01N27/4148 ,  G01N33/54373 ,  G01N33/6818 ,  H01L29/78 ,  H01L29/788 ,  H01L2924/01006

Abstract: Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes.

公开(公告)号：US08890216B2

公开(公告)日：2014-11-18

申请号：US13909290

申请日：2013-06-04

Applicant: Life Technologies Corporation

Inventor： Jonathan M. Rothberg ,  Wolfgang Hinz ,  Kim L. Johnson

IPC: H01L27/00 ,  H01L51/00 ,  G01N27/00 ,  C12Q1/68 ,  G01N27/414 ,  H01L23/00 ,  H01L21/306 ,  H01L29/78

CPC classification number: C12Q1/6869 ,  C12Q1/6874 ,  G01N27/414 ,  G01N27/4145 ,  G01N27/4148 ,  H01L21/306 ,  H01L24/18 ,  H01L24/20 ,  H01L24/82 ,  H01L27/088 ,  H01L29/78 ,  H01L2224/04105 ,  H01L2224/16 ,  H01L2224/76155 ,  H01L2224/82102 ,  H01L2924/01005 ,  H01L2924/01006 ,  H01L2924/01011 ,  H01L2924/01012 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01019 ,  H01L2924/0102 ,  H01L2924/01023 ,  H01L2924/01024 ,  H01L2924/01027 ,  H01L2924/01029 ,  H01L2924/0103 ,  H01L2924/01033 ,  H01L2924/01046 ,  H01L2924/01047 ,  H01L2924/01052 ,  H01L2924/01056 ,  H01L2924/01059 ,  H01L2924/01073 ,  H01L2924/01074 ,  H01L2924/01075 ,  H01L2924/01082 ,  H01L2924/1306 ,  H01L2924/13091 ,  H01L2924/14 ,  H01L2924/1433 ,  H01L2924/19041 ,  H01L2924/19042 ,  H01L2924/19043 ,  H01L2924/30105 ,  H01L2924/3011 ,  H01L2924/3025 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2533/101 ,  H01L2924/00

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

Abstract translation: 与用于分析物测量的非常大规模的FET阵列相关的方法和装置。 可以使用基于提高测量灵敏度和精度的改进的FET像素和阵列设计的传统CMOS处理技术来制造ChemFET（例如，ISFET）阵列，并且同时促进显着小的像素尺寸和致密阵列。 改进的阵列控制技术提供了从大型和密集阵列的快速数据采集。 可以使用这样的阵列来检测各种化学和/或生物过程中各种分析物类型的存在和/或浓度变化。 在一个实例中，chemFET阵列基于监测氢离子浓度（pH），其他分析物浓度变化和/或与DNA合成相关的化学过程相关联的结合事件的变化来促进DNA测序技术。

公开(公告)号：US12146853B2

公开(公告)日：2024-11-19

申请号：US18536131

申请日：2023-12-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James Bustillo ,  Mark J. Milgrew ,  Wolfgang Hinz ,  John Leamon ,  John Davidson ,  Martin Huber ,  Antoine M. van Oijen ,  Jonathan Rothberg

IPC: G01N27/414 ,  C12Q1/6869 ,  C12Q1/6874 ,  H01L21/82

Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

公开(公告)号：US12122859B2

公开(公告)日：2024-10-22

申请号：US18101785

申请日：2023-01-26

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Grete Irene Modahl ,  Nini Hofsloekken Kjus ,  Astrid Evenroed Molteberg ,  Diem Thuy Thi Tran ,  Jo Aaserud ,  M. Talha Gokmen ,  Steven M. Menchen ,  Carl Fuller ,  Luisa Andruzzi ,  Wolfgang Hinz

IPC: C08F230/08 ,  C07F7/18 ,  C08F2/20 ,  C08F2/26 ,  C08F6/24 ,  C08F8/12 ,  C08F8/32 ,  C08F8/34 ,  C08F130/08 ,  C08F220/58 ,  C08F222/38 ,  C08J9/20 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N27/414 ,  C08F220/60

CPC classification number: C08F130/08 ,  C07F7/1804 ,  C07F7/188 ,  C08F2/20 ,  C08F2/26 ,  C08F6/24 ,  C08F8/12 ,  C08F8/32 ,  C08F8/34 ,  C08F222/385 ,  C08F230/085 ,  C08J9/20 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N27/4145 ,  C08F220/603 ,  C08F2800/20 ,  C08J2343/04 ,  Y10T428/2982 ,  C08F8/12 ,  C08F257/02 ,  C08F8/34 ,  C08F8/12 ,  C08F257/02 ,  C12Q1/686 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/543 ,  C08F257/02 ,  C08F230/085 ,  C08F8/12 ,  C08F230/085 ,  C08F8/34 ,  C08F8/12 ,  C08F230/085 ,  C08F230/085 ,  C08F230/085 ,  C08F230/085 ,  C08F230/085 ,  C08F220/603 ,  C08F230/085 ,  C08F212/36 ,  C08F230/085 ,  C08F222/102

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US11572427B2

公开(公告)日：2023-02-07

申请号：US17165039

申请日：2021-02-02

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Grete Irene Modahl ,  Nini Hofsloekken Kjus ,  Astrid Evenroed Molteberg ,  Diem Thuy Thi Tran ,  Jo Aaserud ,  M. Talha Gokmen ,  Steven M. Menchen ,  Carl Fuller ,  Luisa Andruzzi ,  Wolfgang Hinz

IPC: C08F230/08 ,  C08F220/58 ,  C08F8/34 ,  C08F8/32 ,  C08F8/12 ,  C08F2/20 ,  C08F130/08 ,  C08F6/24 ,  C07F7/18 ,  C12Q1/686 ,  C08F2/26 ,  C08F222/38 ,  C12P19/34 ,  C12Q1/6874 ,  G01N27/414 ,  C08J9/20 ,  C08F220/60

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US20210047686A1

公开(公告)日：2021-02-18

申请号：US16882414

申请日：2020-05-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. Rothberg ,  Wolfgang Hinz ,  Kim L. Johnson ,  James Bustillo ,  John Leamon ,  Jonathan Schultz

IPC: C12Q1/6874 ,  G01N27/414 ,  B01L3/00 ,  C12Q1/6869

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.

公开(公告)号：US10605770B2

公开(公告)日：2020-03-31

申请号：US15831141

申请日：2017-12-04

Applicant: Life Technologies Corporation

Inventor： Wolfgang Hinz ,  John Matthew Mauro ,  Shifeng Li ,  James Bustillo

IPC: G01N1/00 ,  G01N27/414 ,  G01N27/26 ,  C12Q1/6874 ,  H01L29/78

Abstract: The described embodiments may provide a method of fabricating a chemical detection device. The method may comprise forming a microwell above a CMOS device. The microwell may comprise a bottom surface and sidewalls. The method may further comprise applying a first chemical to be selectively attached to the bottom surface of the microwell, forming a metal oxide layer on the sidewalls of the microwell, and applying a second chemical to be selectively attached to the sidewalls of the microwell. The second chemical may lack an affinity to the first chemical.

公开(公告)号：US10202473B2

公开(公告)日：2019-02-12

申请号：US15005420

申请日：2016-01-25

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Grete Irene Modahl ,  Nini Hofsloekken Kjus ,  Astrid Evenroed Molteberg ,  Diem Thuy Thi Tran ,  Jo Aaserud ,  M. Talha Gokmen ,  Steven M. Menchen ,  Carl Fuller ,  Luisa Andruzzi ,  Wolfgang Hinz

IPC: C08F130/08 ,  C08F6/24 ,  C07F7/18 ,  C08F2/20 ,  C08F2/26 ,  C08F220/58 ,  C08F230/08 ,  C08F222/38 ,  C08F8/32 ,  C08F8/34 ,  C12Q1/686 ,  C12P19/34 ,  C12Q1/6874 ,  G01N27/414 ,  C08J9/20 ,  C08F8/12

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US09989489B2

公开(公告)日：2018-06-05

申请号：US15427047

申请日：2017-02-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo

IPC: G01N27/403 ,  G01N27/414 ,  C12Q1/68 ,  G01N33/543 ,  H01L29/423

CPC classification number: G01N27/4148 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N33/54373 ,  G01N33/5438 ,  H01L27/088 ,  H01L29/42324 ,  H01L29/78 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01073 ,  H01L2924/14 ,  H01L2924/1433 ,  Y10T436/22 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2533/101

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.