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    METHODS FOR HEAT-ASSISTED ENZYME DIGESTION
    11.
    发明申请
    METHODS FOR HEAT-ASSISTED ENZYME DIGESTION 有权

    公开(公告)号：US20210347814A1

    公开(公告)日：2021-11-11

    申请号：US17314541

    申请日：2021-05-07

    Applicant: Waters Technologies Corporation

    Inventor： Wenjing Li ,  Beatrice Muriithi ,  Anna Boardman ,  Matthew A. Lauber

    IPC: C07K1/12 ,  G01N33/68

    Abstract: The present disclosure relates to a method of digesting a sample comprising protein, the method comprising: adding the sample to an apparatus containing a buffer and a solid support surface comprising a surface coating, wherein the surface coating immobilizes enzyme while reducing undesired interactions between the sample and the solid support surface; immobilizing enzymes on the surface coating for digestion of the protein of the sample; and heating the sample to complete a heat-assisted digestion of the protein.

    LOW BINDING SURFACES FOR PEPTIDE MAPPING
    12.
    发明申请
    LOW BINDING SURFACES FOR PEPTIDE MAPPING 有权

    公开(公告)号：US20210255196A1

    公开(公告)日：2021-08-19

    申请号：US17151036

    申请日：2021-01-15

    Applicant: Waters Technologies Corporation

    Inventor： Robert Birdsall ,  Jacob Kellett ,  Nilini Ranbaduge ,  Ying Qing Yu ,  Jennifer M. Nguyen ,  Matthew A. Lauber

    IPC: G01N33/68 ,  G01N30/72 ,  B01J20/281

    Abstract: The present disclosure discusses a method of separating a sample (e.g., peptide compound) including coating a flow path of a chromatographic system; injecting the sample into the chromatographic system; flowing the sample through the chromatographic system; separating the sample; and analyzing the separated sample. In some examples, the coating applied to the surfaces defining the flow path is non-binding with respect to the sample—and the separated sample. Consequently, the sample does not bind to the low-binding surface of the coating (e.g., organosilica coating) of the flow path. The applied coating can reduce peak tailing and increase analyte recovery for the sample of the chromatographic system.

    METHODS FOR THE RAPID PREPARATION OF LABELED GLYCOSYLAMINES AND FOR THE ANALYSIS OF GLYCOSYLATED BIOMOLECULES PRODUCING THE SAME
    17.
    发明申请
    METHODS FOR THE RAPID PREPARATION OF LABELED GLYCOSYLAMINES AND FOR THE ANALYSIS OF GLYCOSYLATED BIOMOLECULES PRODUCING THE SAME 审中-公开

    公开(公告)号：US20180188259A1

    公开(公告)日：2018-07-05

    申请号：US15522908

    申请日：2015-10-28

    Applicant: Waters Technologies Corporation

    Inventor： Matthew A. Lauber ,  Darryl W. Brousmiche ,  Steven M. Koza

    IPC: G01N33/58 ,  G01N33/68

    CPC classification number: G01N33/58 ,  G01N33/582 ,  G01N33/6842 ,  G01N2333/924 ,  G01N2333/98 ,  G01N2440/38

    Abstract: Methods of analyzing glycosylated biomolecules include the steps of producing a deglycosylation mixture of biomolecules deglycosylated by natural or synthetic enzymatic or chemical techniques; providing a reagent solution having a labeling reagent in a polar aprotic, non-nucleophilic organic solvent; and mixing the deglycosylation mixture with the reagent solution in an excess of labeling reagent to produce derivatized glycosylamines. The method steps can be carried out purposefully without depletion of protein matter. A quenching solution can be added to the reaction mixture so that the pH of the reaction mixture is shifted to above 10. The yield of derivatized glycosylamines can be in an amount of about 80 to about 100 mole percent of the reaction mixture with minimal overlabeling, less than 0.2 mole percent. The derivizated glycosylamines can be separated from the reaction mixture and detected by chromatographic detection, fluorescence detection, mass spectrometry (“MS”), or Ultra Violet (“UV”) detection and/or a combination thereof.

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