公开(公告)号：US09134300B2

公开(公告)日：2015-09-15

申请号：US14306898

申请日：2014-06-17

Applicant: Research Foundation of the City University of New York

Inventor： Ioannis Danias ,  Hyungsik Lim

IPC: G01N33/50 ,  G01J9/00 ,  G01J4/00 ,  G01N33/483 ,  G01N21/63 ,  G01N21/17 ,  G01N21/21 ,  A61B5/00 ,  A61B3/10 ,  G02B21/00

CPC classification number: G01N33/5044 ,  A61B3/10 ,  A61B5/4848 ,  G01J4/00 ,  G01J9/00 ,  G01J2009/002 ,  G01J2009/004 ,  G01N21/1717 ,  G01N21/21 ,  G01N21/63 ,  G01N21/636 ,  G01N33/4833 ,  G01N33/5008 ,  G01N2021/1734 ,  G02B21/002

Abstract: The subject matter disclosed herein pertains to a method for screening drugs using second-harmonic generation microscopy. The tissue is scanned with a pulsed laser light which has an excitation wavelength. At least some of microtubules within the tissue produce generated light with a second-harmonic wavelength that is half the excitation wavelength. A microtubule pattern within the tissue is determined based on an analysis of the generated second-harmonic wavelength.

Abstract translation: 本文公开的主题涉及使用二次谐波生成显微术来筛选药物的方法。 组织用具有激发波长的脉冲激光扫描。 组织内的至少一些微管产生产生的光，其二次谐波波长是激发波长的一半。 基于生成的二次谐波波长的分析来确定组织内的微管图案。

公开(公告)号：US5078491A

公开(公告)日：1992-01-07

申请号：US515729

申请日：1990-04-26

Applicant: Thomas F. Johnston, Jr.

Inventor： Thomas F. Johnston, Jr.

IPC: G01J1/42 ,  G01J9/00

CPC classification number: G01J1/4257 ,  G01J2001/4261 ,  G01J2009/004

Abstract: A method and apparatus for determining the quality of a multimode laser beam (10). In the preferred embodiment, the apparatus includes a lens (32) for creating a transformed or imaged waist from the input beam. The location of the transformed beam waist and its diameter are then determined. These steps can be carried out by chopping the beam using a rotating hub (34) having apertures (36,38) for selectively passing the beam. Preferably, one of the apertures has a pair of 45.degree. knife edges (40,42). The transmission of the beam past the knife edge is monitored by a detector (30). In operation, the lens focal position is varied while the transmission of the beam past the knife edge is monitored in order to locate and measure the diameter of the transformed waist. The diameter of the beam at one other known location is measured. A processor then calculates beam quality by fitting these measurements to a mathematical model. Once the quality of the beam is calculated, the location of the original beam waist and its diameter can be derived. Once all of the beam parameters are derived, the propagation characteristics of the beam can be predicted. In one embodiment of the subject invention, the device includes a blocking member (602) having a knife edge (604) disposed perpendicularly to one of the knife edges of the apertures carried on the hub. The blocking member is translated in discrete steps for each revolution of the hub. The measurement of the power transmitted past the knife edge and blocking member can be used to generate a two-dimensional pixel map of beam intensity.

Abstract translation: 一种用于确定多模激光束（10）的质量的方法和装置。 在优选实施例中，该装置包括用于从输入光束产生变形或成像的腰部的透镜（32）。 然后确定变形的束腰的位置及其直径。 这些步骤可以通过使用具有用于选择性地通过光束的孔（36,38）的旋转毂（34）斩波来进行。 优选地，一个孔具有一对45°刀刃（40,42）。 通过检测器（30）监测光束通过刀刃的透射。 在操作中，透镜焦点位置变化，同时监测光束穿过刀刃的透射，以定位和测量变形腰部的直径。 测量另一个已知位置处的梁的直径。 然后，处理器通过将这些测量拟合到数学模型来计算光束质量。 一旦计算了光束的质量，就可以得出原始光束腰部的位置及其直径。 一旦导出了所有的光束参数，就可以预测光束的传播特性。 在本发明的一个实施例中，该装置包括阻挡构件（602），其具有垂直于轮毂上承载的孔的刀刃之一设置的刀刃（604）。 对于轮毂的每一转，阻挡构件以离散的步骤平移。 通过刀刃和阻挡构件传递的功率的测量可用于产生束强度的二维像素图。

公开(公告)号：US5069527A

公开(公告)日：1991-12-03

申请号：US511817

申请日：1990-04-26

Applicant: Thomas F. Johnston, Jr. ,  David A. Dewey

Inventor： Thomas F. Johnston, Jr. ,  David A. Dewey

IPC: B23K26/02 ,  B23K26/42 ,  G01J1/42 ,  G01J9/00 ,  G02B26/04 ,  H01S3/00

CPC classification number: H01S3/0014 ,  B23K26/02 ,  B23K26/042 ,  B23K26/705 ,  G01J1/4257 ,  G02B26/04 ,  G01J2009/004

Abstract: A method and apparatus for determining the quality of a multimode laser beam (10). In the preferred embodiment, the apparatus includes a lens (32) for creating a transformed or imaged waist from the input beam. The location of the transformed beam waist and its diameter are then determined. These steps can be carried out by chopping the beam using a rotating hub (34) having apertures (36, 38) for seletively passing the beam. Preferably, one of the appertures has a pair of 45.degree. knife edges (40, 42). The transmission of the beam past the knife edge is monitored by a detector (30). In operation, the lens focal position is varied while the transmission of the beam past the knife edge is monitored in order to locate and measure the diameter of the transformed waist. The diameter of the beam at one other known location is measured. A processor then calculates beam quality by fitting these measurements to a mathematical model. Once the quality of the beam is calculated, the location of the original beam waist and its diameter can be derived. Once all of the beam parameters are derived, the propagation characteristics of the beam can be predicted. In the preferred embodiment, the lens is defined by a doublet (533, 535) designed to minimize spherical aberration. In addition, the spacing between the lens pair is varied to minimize the net aberration at the operating wavelength of the laser.

Abstract translation: 一种用于确定多模激光束（10）的质量的方法和装置。 在优选实施例中，该装置包括用于从输入光束产生变形或成像的腰部的透镜（32）。 然后确定变形的束腰的位置及其直径。 这些步骤可以通过使用具有孔（36,38）的旋转轮毂（34）来切割梁来进行，用于使梁完全通过。 优选地，一个孔径具有一对45°刀刃（40,42）。 通过检测器（30）监测光束通过刀刃的透射。 在操作中，透镜焦点位置变化，同时监测光束穿过刀刃的透射，以定位和测量变形腰部的直径。 测量另一个已知位置处的梁的直径。 然后，处理器通过将这些测量拟合到数学模型来计算光束质量。 一旦计算了光束的质量，就可以得出原始光束腰部的位置及其直径。 一旦导出了所有的光束参数，就可以预测光束的传播特性。 在优选实施例中，透镜由设计成使球面像差最小化的双峰（533,535）限定。 另外，改变透镜对之间的间距以使激光器的工作波长处的净像差最小化。