公开(公告)号：US08265883B2

公开(公告)日：2012-09-11

申请号：US13070273

申请日：2011-03-23

Applicant: Stephen J. Gunstream

Inventor： Stephen J. Gunstream

IPC: G06F19/00

CPC classification number: C12Q1/6851 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2201/12 ,  G01N2201/12746 ,  G06F19/22 ,  C12Q2545/113 ,  C12Q2545/101

Abstract: Systems and methods are provided for calibrating emission data or other information signals collected during a polymerase chain reaction (PCR), amplification reaction, assay, process, or other reaction. Calibration of multiple detectable materials can be achieved during a single cycle or run, or during a plurality of runs of the reaction. A reading from every well, container, or other support region of a sample support does not have to be taken. Interpolation can be used to determine values for emission data or other information signals that were not taken, or are unknown, using detected emission data, or other detected information signals. By calibrating the detected emission data and the interpolated data, a more accurate reading of emission data or information signal can be obtained.

Abstract translation: 提供了系统和方法，用于校准在聚合酶链式反应（PCR），扩增反应，测定，过程或其他反应期间收集的排放数据或其他信息信号。 可以在单个循环或运行期间或在多次反应运行期间实现多个可检测材料的校准。 来自样品支架的每个孔，容器或其他支撑区域的读数不必采取。 内插可用于确定未使用或未知的使用检测到的发射数据或其他检测到的信息信号的发射数据或其他信息信号的值。 通过校准检测到的发射数据和内插数据，可以获得更准确的发射数据或信息信号读数。

公开(公告)号：US20100268504A1

公开(公告)日：2010-10-21

申请号：US12798290

申请日：2010-04-01

Applicant: Garland Christian Misener

Inventor： Garland Christian Misener

IPC: G06F19/00

CPC classification number: G01N21/55 ,  G01N21/274 ,  G01N21/59 ,  G01N2201/062 ,  G01N2201/12746

Abstract: A method of deriving correction for instrument-to-instrument variations in the illumination band centroid wavelengths and wavelength band shapes of the optical systems of clinical chemistry instruments includes the steps of determining the centroid wavelength and wavelength band shape of a light source used in the optical system of a clinical chemistry instrument to provide a determined wavelength band shape and centroid wavelength, comparing the determined wavelength band shape and centroid wavelength with a known reflection density or absorbance wavelength spectrum of a specific type of chemical reagent test to provide a correction value, and calculating the correction value, which is to be used to modify a reflection density or absorbance measurement taken by the instrument of a reagent test of the a specific type of chemical reagent test.

Abstract translation: 导出临床化学仪器的光学系统的照明带质心波长和波长带形状中的仪器与仪器变化的校正方法包括以下步骤：确定在光学器件中使用的光源的质心波长和波长带形状 系统，以提供确定的波长带形状和质心波长，将确定的波长带形状和质心波长与特定类型化学试剂测试的已知反射密度或吸光度波长光谱进行比较以提供校正值，以及 计算校正值，该校正值用于修改由特定类型的化学试剂试剂的试剂测试所采用的反射浓度或吸光度测量值。

公开(公告)号：US07523637B2

公开(公告)日：2009-04-28

申请号：US11947116

申请日：2007-11-29

Applicant: Wayne D. Roth ,  Douglas E. Moore

Inventor： Wayne D. Roth ,  Douglas E. Moore

IPC: G01N21/00

CPC classification number: G01N15/1404 ,  G01N15/1012 ,  G01N21/645 ,  G01N2015/1415 ,  G01N2015/1477 ,  G01N2201/12746

Abstract: Various methods for controlling one or more parameters of a flow cytometer type measurement system are provided. One embodiment includes monitoring parameter(s) of the measurement system during measurements of sample microspheres. The method also includes altering the parameter(s) in real time based on the monitoring. Another method includes monitoring a temperature proximate to the measurement system. One such method includes altering a bias voltage of an avalanche photo diode in response to the temperature using empirically derived data. A different such method includes altering output signals of a photomultiplier tube in response to the temperature using a characteristic curve. Some methods include monitoring a temperature of a fluid, in which sample microspheres are disposed, that will flow through the flow cytometer type measurement system. This method also includes determining a velocity of the sample microspheres in the measurement system from a viscosity of the fluid at the temperature.

Abstract translation: 提供了用于控制流式细胞仪型测量系统的一个或多个参数的各种方法。 一个实施例包括在样品微球的测量期间监测测量系统的参数。 该方法还包括基于监视实时更改参数。 另一种方法包括监测靠近测量系统的温度。 一种这样的方法包括使用经验导出的数据来响应于温度来改变雪崩光电二极管的偏置电压。 不同的这种方法包括使用特性曲线响应于温度改变光电倍增管的输出信号。 一些方法包括监测将流过流式细胞仪类型测量系统的流体样品微球的设置温度。 该方法还包括从该温度下的流体的粘度确定测量系统中的样品微球的速度。

公开(公告)号：US20080209978A1

公开(公告)日：2008-09-04

申请号：US12022098

申请日：2008-01-29

Applicant: Stephen J. Gunstream

Inventor： Stephen J. Gunstream

IPC: G12B13/00

CPC classification number: C12Q1/6851 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2201/12 ,  G01N2201/12746 ,  G16B30/00 ,  C12Q2545/113 ,  C12Q2545/101

Abstract: Methods for generating a calibration factor and for calibrating are provided whereby interstitial spacing between support locations of a platform is used to embed a detectable material, emissions from which are used to generate the calibration factor. In some embodiments, the external space, outer perimeter, and other areas of a platform are used to embed detection materials for the normalization, calibration, correction, compensation, or other method of adjustment for detected emission data. The emission data can be taken from an assay, amplification, reaction, analysis, or other process, for example, from a PCR run or other reaction. By calibrating or adjusting the sample support with the detected emission data, a more efficient detection of the assay, amplification, reaction, analysis, or other process, can be achieved.

Abstract translation: 提供用于产生校准因子和校准的方法，由此使用平台的支撑位置之间的间隙来嵌入可检测材料，用于产生校准因子的发射。 在一些实施例中，平台的外部空间，外部周边和其他区域用于嵌入检测材料，用于检测发射数据的归一化，校准，校正，补偿或其他调整方法。 发射数据可以从测定，扩增，反应，分析或其它过程中获得，例如，从PCR运行或其他反应。 通过使用检测到的排放数据校准或调整样品支架，可以实现更有效的检测，扩增，反应，分析或其他过程。

公开(公告)号：US20080182264A1

公开(公告)日：2008-07-31

申请号：US12022087

申请日：2008-01-29

Applicant: Stephen J. Gunstream

Inventor： Stephen J. Gunstream

IPC: C12Q1/68 ,  C12M1/00 ,  G06G7/00

CPC classification number: C12Q1/6851 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2201/12 ,  G01N2201/12746 ,  G06F19/22 ,  C12Q2545/113 ,  C12Q2545/101

Abstract: Systems and methods for normalizing detected emission data collected in real-time polymerase chain reaction (RT-PCR) and other reactions, are provided. In some embodiments, a sample plate can be loaded with a fluorescent dye and subjected to a real-time PCR reaction. During the initial cycles, detected emissions that correspond to the background signal contributed by the plate, buffer, and other non-reactant pieces of the reaction system and chemistry can be identified. The raw emission data can be normalized by dividing the emission data by the identified baseline signal. According to various embodiments, the normalized amplification profile can normalize to an initial value of 1, because the actual signal emerges from the baseline at the point exponential growth begins. A normalized amplification profile based on a ratio to the baseline can create a more uniformly scaled amplification curve across different samples, filters, wells, dyes, or machines.

Abstract translation: 提供了在实时聚合酶链反应（RT-PCR）和其他反应中收集的检测到的排放数据归一化的系统和方法。 在一些实施方案中，样品板可以装载荧光染料并进行实时PCR反应。 在初始循环期间，可以鉴定出与反应体系和化学物质的板，缓冲液和其它非反应物片段所贡献的背景信号相对应的检测到的排放物。 可以通过将发射数据除以所识别的基线信号来对原始发射数据进行归一化。 根据各种实施例，归一化放大曲线可以归一化为初始值1，因为实际信号在指数增长点开始从基线出现。 基于与基线的比率的归一化扩增曲线可以在不同样品，滤器，孔，染料或机器上产生更均匀的缩放扩增曲线。

公开(公告)号：US20040263837A1

公开(公告)日：2004-12-30

申请号：US10608248

申请日：2003-06-27

Applicant: Meyer Tool, Inc.

Inventor： Dwight Evans Kimberlin

IPC: G01N021/00

CPC classification number: G01N21/954 ,  G01N21/8806 ,  G01N21/894 ,  G01N21/95692 ,  G01N2201/12746

Abstract: A hole inspection system having a light source emitting light over its length and a multi-axes machine having a camera mounted thereon. After the light source is inserted into a cavity intersecting the complex holes, a control commands the multi-axes machine to move the camera to an inspection position associated with one of the complex holes. The control processes substantially only light intensity values received from the camera that represent light shining through the one of the complex holes. Next, a maximum intensity value of light received by the camera from the one of the complex holes is determined. The maximum intensity value is compared to a threshold value, and error data is created that identifies the one of the complex holes in response to the maximum intensity value being less than the threshold value.

Abstract translation: 一种具有在其长度上发射光的光源的孔检查系统和具有安装在其上的照相机的多轴机器。 在将光源插入到与复合孔相交的空腔中之后，控制命令多轴机器将相机移动到与复合孔中的一个相关联的检查位置。 该控制基本上只处理从照相机接收的光强度值，其表示通过复合孔之一照射的光。 接下来，确定相机从复合孔之一接收的光的最大强度值。 将最大强度值与阈值进行比较，并且创建误差数据，其响应于最大强度值小于阈值来识别复数个孔中的一个。

公开(公告)号：US20240085322A1

公开(公告)日：2024-03-14

申请号：US17940068

申请日：2022-09-08

Applicant: Taiwan RedEye Biomedical Inc.

Inventor： Shuo-Ting Yan ,  I-Hua Wang ,  Chen-Chung Chang

IPC: G01N21/53 ,  A61B5/00 ,  A61B5/145 ,  A61B5/1455 ,  A61B5/1495 ,  E03D11/00

CPC classification number: G01N21/53 ,  A61B5/0004 ,  A61B5/14546 ,  A61B5/1455 ,  A61B5/1495 ,  A61B5/6891 ,  E03D11/00 ,  A61B2562/0238 ,  G01N2201/12746

Abstract: The present invention relates to an optical system for triglyceride inspection partially integrated into a toilet seat and comprising a plurality of optical sensor modules and a controlling and processing module, wherein each said optical sensor module comprises a first light source, a second light source and an optical sensor. The optical sensor receives light signals generated by the first and second light sources respectively on the skin of the person (especially the skin of the thighs) to be tested and thereby generates a sensing signal of an adaptive calibration function. The sensing signal is then converted by the controlling and processing module into an inspection value of triglyceride, which is transmitted to a display unit. With the above optical system for triglyceride inspection, triglycerides can be inspected automatically without invasive blood sampling, making the system a convenient home health monitoring device.

公开(公告)号：US20230333014A1

公开(公告)日：2023-10-19

申请号：US18182010

申请日：2023-03-10

Applicant: Melexis Technologies NV

Inventor： Jos RENNIES ,  Luc BUYDENS

IPC: G01N21/55

CPC classification number: G01N21/55 ,  G01N2201/123 ,  G01N2201/12746

Abstract: In a heating appliance comprising a substrate for receiving an item of cookware, a method of measuring reflectivity comprises emitting a time-varying electromagnetic signal from a first side of the substrate, a portion of the time-varying electromagnetic signal propagating through the substrate. Electromagnetic radiation is then received at the first side of the substrate, the received electromagnetic radiation comprising a background ambient component received and a component reflected by the substrate. A gain factor is applied to translate the received electromagnetic radiation to a receive electrical signal. An offset signal component is then identified from the receive electrical signal, the offset signal component arising from the background ambient component of the received electromagnetic radiation. The gain factor from the offset signal component is then estimated using a characterisation of the offset signal component, and the reflectivity is calculated using the receive electrical signal and the estimated gain factor.

公开(公告)号：US11768160B2

公开(公告)日：2023-09-26

申请号：US17100859

申请日：2020-11-21

Applicant: UNITED STATES GEOLOGICAL SURVEY

Inventor： Brian Anthony Pellerin ,  Angela Merleen Hansen

IPC: G01N21/00 ,  G01N21/93 ,  G01N33/18

CPC classification number: G01N21/93 ,  G01N33/18 ,  G01N2201/12715 ,  G01N2201/12746 ,  G01N2201/13

Abstract: A multiparameter standard solution for verifying and calibrating water quality sensors containing an aqueous pH buffer, a xanthene dye, and distyryl biphenyl (DSBP) is provided. The standard solution provides a safe, quick, easy, and stable field standard to simultaneously conduct calibration analysis for several sensors at once. The standard solution is stable when stored and can be safely disposed of in the field. Methods of calibrating sensors used in water quality analysis using the multiparameter standard solution are also provided.

公开(公告)号：US20230273120A1

公开(公告)日：2023-08-31

申请号：US17682243

申请日：2022-02-28

Applicant: Saudi Arabian Oil Company

Inventor： Mohammad Alharthy ,  Osama Alzahrani ,  Nada Alghamdi ,  NagoorPitchal MeeranPillai ,  Ali Almuhaimeed

IPC: G01N21/33 ,  G01N33/18 ,  G01N1/40

CPC classification number: G01N21/33 ,  G01N33/1833 ,  G01N1/4077 ,  G01N2201/127 ,  G01N2001/4083 ,  G01N2201/12746

Abstract: A method for quantifying a crude oil in water is provided. The method includes selecting an ultraviolet/visible (UV/Vis) wavelength to perform a measurement, preparing calibration solutions in xylene, and preparing a calibration curve from the calibration solutions. A sample is prepared including extracting the crude oil from the water in a two-phase separation with xylene. An absorbance of the sample in the xylene is measured at the UV/Vis wavelength. A concentration of the crude oil in the water is calculated from the absorbance.