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公开(公告)号：US09050606B2
公开(公告)日：2015-06-09
申请号：US14491231
申请日：2014-09-19
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Vamsee K. Pamula , Arjun Sudarsan , Ramakrishna Sista
CPC classification number: B03C7/026 , B01L3/50273 , B01L3/502761 , B01L2200/0647 , B01L2300/0887 , B01L2400/0424 , B01L2400/0427 , B03C1/01 , B03C1/288 , B03C5/00 , B03C5/005 , B03C5/026 , B03C2201/26 , G01N33/54326
Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.
Abstract translation: 本发明提供了一种在液滴中重新分布磁响应珠的方法。 该方法可以包括在液滴操作表面上使用液滴进行一个或多个液滴操作，而不从磁场的区域移除磁响应珠。 在某些情况下，液滴操作可能是电极介导的。 液滴操作可以在液滴内重新分布和/或循环磁响应珠。 在一些情况下，液滴可以包括样品液滴可以包括靶分析物。 磁响应珠的重新分布可能导致目标分析物结合磁响应珠。 在一些情况下，液滴可以包括洗涤缓冲液中的未结合物质。 磁响应珠子的重新分布导致未结合物质从磁性响应珠粒的聚集或子集的空隙中释放出来。
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公开(公告)号：US20150075985A1
公开(公告)日：2015-03-19
申请号：US14541825
申请日：2014-11-14
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Michael G. Pollack , Vamsee K. Pamula , Vijay Srinivasan
IPC: B01L3/00 , G01N27/447
CPC classification number: B01L3/502761 , B01F13/0071 , B01F13/0076 , B01L3/502792 , B01L2200/0605 , B01L2200/0647 , B01L2200/0668 , B01L2200/16 , B01L2300/0819 , B01L2300/0864 , B01L2300/0867 , B01L2300/088 , B01L2300/089 , B01L2400/0427 , B01L2400/043 , G01N27/447
Abstract: The invention provides nonlimiting examples of structures for and methods of dispensing droplets in a droplet actuator. The droplet actuator structures and methods of the invention exhibit numerous advantages over droplet actuators of the prior art. In various embodiments, the structures and methods of the invention provide, among other things, improved efficiency, throughput, scalability, and/or droplet uniformity, as compared with existing droplet actuators. Further, in some embodiments, the droplet actuators provide configurations for improved methods of loading and/or unloading fluid and/or droplets. In yet other embodiments, the droplet actuators provide fluid loading configurations for loading numerous fluid reservoirs in a substantially simultaneous and/or substantially sequential manner.
Abstract translation: 本发明提供了用于在液滴致动器中分配液滴的结构的方法和方法的非限制性实例。 本发明的液滴致动器结构和方法与现有技术的液滴致动器相比表现出许多优点。 在各种实施例中，与现有的液滴致动器相比，本发明的结构和方法尤其提供了改进的效率，吞吐量，可扩展性和/或液滴均匀性。 此外，在一些实施例中，液滴致动器提供用于加载和/或卸载流体和/或液滴的改进方法的构造。 在其他实施例中，液滴致动器提供用于以基本上同时和/或基本顺序的方式装载多个流体储存器的流体加载构造。
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公开(公告)号：US08980198B2
公开(公告)日：2015-03-17
申请号：US11639594
申请日：2006-12-15
Applicant: Vijay Srinivasan , Michael G. Pollack , Philip Y. Paik , Vamsee K. Pamula , Richard B. Fair
Inventor： Vijay Srinivasan , Michael G. Pollack , Philip Y. Paik , Vamsee K. Pamula , Richard B. Fair
IPC: B01L3/00 , G01N27/447 , B01F13/00 , B01L7/00 , G01N35/10
CPC classification number: B01L3/502792 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L7/52 , B01L7/525 , B01L7/54 , B01L2200/0647 , B01L2200/0673 , B01L2200/10 , B01L2200/147 , B01L2300/0816 , B01L2300/0861 , B01L2300/089 , B01L2300/1827 , B01L2400/0427 , B01L2400/0448 , B03C5/02 , B81B1/00 , G01N27/447 , G01N2035/1046
Abstract: The present invention relates to filler fluids for droplet operations. According to one embodiment of this aspect, a droplet microactuator is provided and includes: (a) a first substrate comprising electrodes configured for conducting droplet operations on a surface of the substrate; (b) a second substrate spaced from the surface of the substrate by a distance sufficient to define an interior volume between the first substrate and second substrate, wherein the distance is sufficient to contain a droplet disposed in the space on the first substrate; and (c) a droplet arranged in the interior volume and arranged with respect to the electrodes in a manner which permits droplet operations to be effected on the droplet using the electrodes.
Abstract translation: 本发明涉及用于液滴操作的填充流体。 根据该方面的一个实施例，提供了液滴微致动器，其包括：（a）第一基板，其包括被配置用于在所述基板的表面上进行液滴操作的电极; （b）第二衬底，其与衬底的表面间隔足以限定第一衬底和第二衬底之间的内部空间的距离，其中所述距离足以容纳设置在第一衬底上的空间中的液滴; 和（c）布置在内部体积中并且以允许使用电极对液滴进行液滴操作的方式相对于电极布置的液滴。
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公开(公告)号：US20150027889A1
公开(公告)日：2015-01-29
申请号：US14506265
申请日：2014-10-03
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Michael G. Pollack , Prasanna Thwar , Vamsee K. Pamula , Vijay Srinivasan , Allen E. Eckhardt , Alexander Shenderov , Dwayne J. Allen
IPC: B01L3/00 , G01N27/447
CPC classification number: B01L3/502784 , B01L3/502715 , B01L3/502761 , B01L7/52 , B01L2200/027 , B01L2200/0673 , B01L2300/022 , B01L2300/023 , B01L2300/024 , B01L2300/0672 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0478 , B01L2400/0481 , B01L2400/0487 , B01L2400/049 , B01L2400/0683 , G01N27/44704 , G01N35/0098 , G01N2035/1046 , Y10T436/25 , Y10T436/2575
Abstract: The invention provides droplet actuators and droplet actuator cassettes including reagent storage capabilities, as well as methods of making and using the droplet actuators and cassettes. The invention also provides continuous flow channel elements and techniques for using electrodes to manipulate droplets in flowing streams. The invention also discloses methods of separating compounds on a droplet actuator. Various other aspects of the invention are also disclosed.
Abstract translation: 本发明提供了包括试剂储存能力的液滴致动器和液滴致动器盒，以及制造和使用液滴致动器和盒的方法。 本发明还提供了用于使用电极在流动流中操纵液滴的连续流动通道元件和技术。 本发明还公开了在液滴致动器上分离化合物的方法。 还公开了本发明的各种其它方面。
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公开(公告)号：US20140216932A1
公开(公告)日：2014-08-07
申请号：US14248884
申请日：2014-04-09
Applicant: Advanced Liquid Logic, Inc.
Inventor： Vijay Srinivasan , Michael G. Pollack , Alexander Shenderov , Zhishan Hua , Arjun Sudarsan
IPC: B01L3/00
CPC classification number: B01L3/502792 , B01L2200/12 , B01L2300/0819 , B01L2300/089 , B01L2400/0415 , B01L2400/0421 , B01L2400/0427 , Y10T29/49826
Abstract: The invention provides a droplet actuator. The droplet actuator may include a base substrate and a top substrate separated to form a gap. The base substrate may include electrodes configured for conducting droplet operations in the gap; and the top substrate may include a glass substrate portion coupled to a non-glass portion, where the non-glass portion may include one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap. The invention also provides related methods of manufacturing the droplet actuator, methods of using the droplet actuator, and methods of loading the droplet actuator.
Abstract translation: 本发明提供一种液滴致动器。 液滴致动器可以包括分离以形成间隙的基底基板和顶基板。 基底基板可以包括被配置用于在间隙中进行液滴操作的电极; 并且顶部衬底可以包括联接到非玻璃部分的玻璃衬底部分，其中非玻璃部分可以包括建立从液滴致动器的外部延伸到间隙中的流体路径的一个或多个开口。 本发明还提供了制造液滴致动器的相关方法，使用液滴致动器的方法以及装载液滴致动器的方法。
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Patent Agency Ranking
公开(公告)号：US20140057363A9
公开(公告)日：2014-02-27
申请号：US12992939
申请日：2009-05-18
Applicant: Ramakrishna Sista , Vamsee Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar
Inventor： Ramakrishna Sista , Vamsee Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar
IPC: G01N1/40
CPC classification number: B01L3/502792 , B01L3/502761 , B01L2200/0673 , B01L2300/0816 , B01L2300/0819 , B01L2300/0829 , B01L2300/089 , B01L2400/0406 , B01L2400/0415 , B01L2400/0427 , B01L2400/043 , B01L2400/0487 , G01N1/4077 , G01N35/0098 , G01N2035/00564 , Y10T436/25
Abstract: The invention provides droplet actuators and droplet actuator techniques. Among other things, the droplet actuators and methods are useful for manipulating beads on a droplet actuator, such as conducting droplet operations using bead-containing droplets on a droplet actuator. For example, beads may be manipulated on a droplet actuator in the context of executing a sample preparation protocol and/or an assay protocol. An output of the methods of the invention may be beads prepared for execution of an assay protocol. Another output of the methods of the invention may be results of an assay protocol executed using beads. Among the methods described herein are methods of concentrating beads in droplets, methods of washing beads, methods of suspending beads, methods of separating beads, methods of localizing beads within a droplet, methods of forming emulsions in which droplets include beads, methods of loading beads into a droplet operations gap of a droplet actuator, methods of organizing beads in a monolayer, and methods of capturing, trapping or restraining beads.
Abstract translation: 本发明提供了液滴致动器和液滴致动器技术。 除其他之外，液滴致动器和方法可用于操纵液滴致动器上的珠，例如使用液滴致动器上的含珠液滴进行液滴操作。 例如，在执行样品制备方案和/或测定方案的上下文中，珠可以在液滴致动器上操作。 本发明方法的输出可以是准备用于执行测定方案的珠粒。 本发明方法的另一个输出可以是使用珠子执行的测定方案的结果。 在本文描述的方法中，将珠粒集中在液滴中的方法，洗涤珠粒的方法，悬浮珠粒的方法，分离珠粒的方法，在液滴内定位珠粒的方法，形成液滴包括珠粒的乳液的方法，加载珠粒的方法 液滴致动器的液滴操作间隙，单层组织珠粒的方法，以及捕获，捕获或抑制珠粒的方法。
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公开(公告)号：US20140014517A1
公开(公告)日：2014-01-16
申请号：US14027941
申请日：2013-09-16
Applicant: Advanced Liquid Logic, Inc.
Inventor： Vijay Srinivasan , Michael G. Pollack , Zhishan Hua , Arjun Sudarsan , Allen E. Eckhardt
IPC: G01N27/447
CPC classification number: G01N27/44743 , B01L3/0268 , B01L3/502761 , B01L3/502792 , B01L2200/0605 , B01L2200/061 , B01L2200/0647 , B01L2300/0645 , B01L2300/16 , B01L2400/0415 , B01L2400/0427
Abstract: Droplet actuator for conducting droplet operations, such as droplet transport and droplet dispensing, is provided. In one embodiment, the droplet actuator may include an electrode that is rotationally but not reflectively symmetrical.
Abstract translation: 提供用于进行液滴操作的液滴致动器，例如液滴传输和液滴分配。 在一个实施例中，液滴致动器可以包括旋转但不反射对称的电极。
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28.发明申请公开(公告)号：US20140005066A1
公开(公告)日：2014-01-02
申请号：US13932719
申请日：2013-07-01
Applicant: ADVANCED LIQUID LOGIC INC.
Inventor： Deborah Boles , Lisa Perkins , Allen E. Eckhardt , Jennifer Foley
CPC classification number: C12Q1/703 , C12Q1/686 , C12Q1/701 , C12Q2537/143 , C12Q2561/113 , C12Q2563/107 , C12Q2563/159 , C12Q2565/102
Abstract: The present invention provides a droplet actuator device and methods for multiplexed PCR amplification and detection of target amplicons within a single droplet. The methods of the invention combine quantitative real-time PCR (qPCR) amplification with fluorescence-based sequence specific detection technologies for amplified DNA. In one embodiment, fluorescently-labeled oligonucleotide probes may be used for hybridization-based multiplexed detection of target amplicons. The methods of the invention generally involve combining the necessary reactants to form a PCR-ready droplet and thermal cycling the droplet at temperatures sufficient to result in amplification of one or more target nucleic acids. Fluorescence-based detection techniques may be used for end-point or real-time analysis of DNA amplification. For end-point analysis, the accumulation of a signal, e.g., a fluorescence signal, is measured after the amplification of the target sequence is complete. For real-time analysis, the signal is measured while the amplification reaction is in progress.
Abstract translation: 本发明提供了一种液滴致动器装置和用于在单个液滴内多重PCR扩增和检测靶扩增子的方法。 本发明的方法将定量实时PCR（qPCR）扩增与扩增DNA的基于荧光的序列特异性检测技术相结合。 在一个实施方案中，荧光标记的寡核苷酸探针可用于靶扩增子的杂交多重检测。 本发明的方法通常包括组合必需的反应物以形成PCR准备的液滴并在足以导致一种或多种靶核酸扩增的温度下热循环该液滴。 基于荧光的检测技术可用于DNA扩增的终点或实时分析。 对于终点分析，在靶序列的扩增完成之后测量信号的累积，例如荧光信号。 对于实时分析，在扩增反应进行期间测量信号。
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公开(公告)号：US08592217B2
公开(公告)日：2013-11-26
申请号：US13738259
申请日：2013-01-10
Applicant: Advanced Liquid Logic, Inc.
Inventor： Allen E. Eckhardt
IPC: G01N33/00
CPC classification number: C12Q1/40 , B01L3/502784 , B01L2300/0654 , B01L2300/0816 , B01L2300/18 , B01L2400/0427 , B01L2400/043 , C07D311/16 , C07D311/18 , C07D311/20 , C07D405/12 , C07D413/12 , C07H15/203 , C07H17/075 , C12Q1/25 , C12Q1/34 , C12Q1/44 , C12Q1/54 , C12Y302/01023 , G01N21/6452 , G01N33/5438 , G01N2333/916 , G01N2333/926 , G01N2333/938 , G01N2800/04
Abstract: The invention is directed to droplet actuator devices and assay methods. The invention includes assay methods of conducting an assay comprising combining a sample with an umbelliferyl derivative, wherein the sample potentially comprises an enzyme capable of cleaving the umbelliferyl derivative and where the umbelliferyl derivative comprises an umbelliferyl core modified with one or more modifying moieties.
Abstract translation: 本发明涉及液滴致动器装置和测定方法。 本发明包括进行测定的测定方法，其包括将样品与伞形酮基衍生物组合，其中所述样品潜在地包含能够切割伞形变体基衍生物的酶，并且其中伞形载体衍生物包含用一个或多个修饰部分修饰的伞形酮核心。
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公开(公告)号：US08591830B2
公开(公告)日：2013-11-26
申请号：US12673893
申请日：2008-08-25
Applicant: Arjun Sudarsan , Michael G. Pollack , Prasanna Thwar , Dwayne Allen
Inventor： Arjun Sudarsan , Michael G. Pollack , Prasanna Thwar , Dwayne Allen
IPC: B01L3/00
CPC classification number: B01L3/52 , B01L3/502761 , B01L3/502792 , B01L2200/0668 , B01L2300/0864 , B01L2300/089 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427
Abstract: A droplet actuator comprising: (a) a base substrate comprising electrodes configured for conducting droplet operations on a droplet operations surface thereof; (b) a droplet comprising one or more beads situated on the droplet operations surface; (c) a barrier arranged in relation to the droplet and the electrodes such that a droplet may be transported away from the beads using one or more droplet operations mediated by one or more of the electrodes while transport of the beads is restrained by a barrier. Related methods and kits are also provided.
Abstract translation: 一种液滴致动器，包括：（a）基底基底，包括构造成用于在其液滴操作表面上进行液滴操作的电极; （b）包含位于所述液滴操作表面上的一个或多个珠粒的液滴; （c）相对于液滴和电极布置的屏障，使得可以使用由一个或多个电极介导的一个或多个液滴操作将液滴从珠流出，同时珠的传送被屏障限制。 还提供了相关方法和试剂盒。
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