COMPOSITIONS AND METHODS FOR NUCLEIC ACID DETECTION BY LATERAL FLOW ASSAYS

    公开(公告)号:US20240124917A1

    公开(公告)日:2024-04-18

    申请号:US18546515

    申请日:2022-02-15

    CPC classification number: C12Q1/6816 C12N9/22 C07K2319/80

    Abstract: Rapid, sensitive, and specific point-of-care testing for pathogens is crucial for disease control. Lateral flow assays (LFAs) have been employed for nucleic acid detection, but they have limited sensitivity and specificity. A fusion of catalytically inactive Cas9 endonuclease and a relaxase for example, VirD2 are used for sensitive, specific nucleic acid detection by LFA. VirD2-dCas9 specifically binds the target nucleic acid sequence via dCas9 and covalently binds to a FAM-tagged oligonucleotide via VirD2. The biotin label and FAM tag are detected using a LFA. This system, termed Vigilant (VirD2-dCas9 guided and LFA-coupled nucleic acid test) is coupled to reverse transcription-recombinase polymerase amplification to detect pathogenic nucleic acid of interest in a sample, it exhibits an impressive limit of detection and shows no cross-reactivity, thus reducing incidents of false positives. Vigilant offers an easy-to-use, rapid, cost-effective, and robust detection platform for SARS-CoV2.

    Fast probability measure module for engineering systems and method

    公开(公告)号:US11886537B2

    公开(公告)日:2024-01-30

    申请号:US17670601

    申请日:2022-02-14

    CPC classification number: G06F17/18 G06F11/3452

    Abstract: A method for estimating a status of a system includes performing independent measurements on the system, associating a value P(γ) with a Monte Carlo calculated probability measure P(γ), which depends on (1) a preset threshold Nu of unfavorable events, and (2) a total number Ñtot(γ) of the independent measurements for collecting the preset threshold Nu, setting a threshold level η requiring that a critical value γc of a power related metric γ makes a value P(γc) of the probability measure P to be substantially equal to the threshold level η, comparing the total number Ñtot(γ) to a ratio of (1) a product of a control parameter ϵ the preset threshold Nu, and (2) the threshold level η, and interrupting the independent measurements if Ñtot(γ) is larger than the ratio, or increasing an output of the system if Ñtot(γ) is smaller than or equal to the ratio.

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