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公开(公告)号：US20220082489A1
公开(公告)日：2022-03-17
申请号：US17304844
申请日：2021-06-26
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： Maria Jaimes , Zhenyu Zhang , Jian Zhou , Huiming Gu , Shao Qing , Ming Yan , David Vrane
IPC: G01N15/14
Abstract: In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a full spectrum flow cytometer with five excitation lasers and five corresponding detection modules. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.
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公开(公告)号：US20220082488A1
公开(公告)日：2022-03-17
申请号：US17304843
申请日：2021-06-26
Applicant: Cytek Biosciences, Inc.
Inventor： Maria Jaimes , Zhenyu Zhang , Jian Zhou , Huiming Gu , Shao Qing , Ming Yan , David Vrane
IPC: G01N15/14
Abstract: In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a full spectrum flow cytometer with five excitation lasers and five corresponding detection modules. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.
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公开(公告)号：US20220034783A1
公开(公告)日：2022-02-03
申请号：US17390984
申请日：2021-08-01
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： David Vrane
IPC: G01N15/14
Abstract: A system, method, and apparatus are provided for cytometer fluidics. In one example, a pressure regulation system is provided for a cytometer fluidics system. The pressure regulation system includes a pressure regulator and a transducer. The pressure regulator pressurizes sheath fluid in a fluid path. The transducer senses a measured pressure in the fluid path independently of the sheath tank. The transducer converts the measured pressure into a voltage. The transducer communicates the voltage to the pressure regulator. The pressure regulator translates the voltage to a regulated pressure that substantially matches the measured pressure. The pressure regulator is the only pressure regulation source in the cytometry fluidics system.
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公开(公告)号：US20200333237A1
公开(公告)日：2020-10-22
申请号：US16920618
申请日：2020-07-03
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： David Vrane , Ming Yan , Eric Chase , Wenbin Jiang
Abstract: A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a grating system including a dispersive element and a receiver assembly, wherein the dispersive element is positioned to receive the fluorescent light from the fiber assembly and to direct spectrally dispersed light toward the receiver assembly.
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公开(公告)号：US10739245B2
公开(公告)日：2020-08-11
申请号：US15498397
申请日：2017-04-26
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： David Vrane , Ming Yan , Eric Chase , Wenbin Jiang
Abstract: A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a grating system including a dispersive element and a receiver assembly, wherein the dispersive element is positioned to receive the fluorescent light from the fiber assembly and to direct spectrally dispersed light toward the receiver assembly.
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Patent Agency Ranking
公开(公告)号：US20200049614A1
公开(公告)日：2020-02-13
申请号：US16101385
申请日：2018-08-10
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： Janine Jiang , David Vrane
IPC: G01N15/14
Abstract: In some embodiments, a smart flow cytometer includes a monitoring system to monitor differing operational parameters of the smart flow cytometer to detect an advanced failure of components and an advanced need for maintenance. In others, a smart flow cytometer includes a quality control system including a reservoir of quality control beads to periodically run a validation test with the quality control beads in order to determine the ability of the flow cytometer to generate quality data output from a plurality of light detectors. In some embodiments, a plurality of smart flow cytometers are coupled into communication with a computer communication network; a central repair server system is coupled into communication with the computer communication network and the plurality of smart flow cytometers; wherein each of the plurality of smart flow cytometers includes a monitoring system coupled to monitor differing operational parameters of the smart flow cytometer for possible failure.
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公开(公告)号：US20180156710A1
公开(公告)日：2018-06-07
申请号：US15817237
申请日：2017-11-19
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： David Vrane
IPC: G01N15/14
CPC classification number: G01N15/1404 , G01N15/1434 , G01N15/1436 , G01N15/147 , G01N21/49 , G01N2015/1006 , G01N2015/1075 , G01N2015/1409 , G01N2015/1438
Abstract: A system, method, and apparatus are provided for flow cytometry. In one example, the flow cytometry system includes dual laser devices and dual scatter channels to measure velocity of particles in a core stream of sample fluid. The total flow rate of the sample fluid and the sheath fluid around the sample fluid is controlled, and thus held constant, by a feedback control system controlling a vacuum pump based on differential pressure across ends of a flow channel in the flow cell.
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公开(公告)号：US12209948B2
公开(公告)日：2025-01-28
申请号：US17304843
申请日：2021-06-26
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： Maria Jaimes , Zhenyu Zhang , Jian Zhou , Huimin Gu , Qing Shao , Ming Yan , David Vrane
IPC: G01N15/14 , G01N15/00 , G01N15/1434 , G01N15/01
Abstract: In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a full spectrum flow cytometer with five excitation lasers and five corresponding detection modules. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.
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公开(公告)号：US11965813B2
公开(公告)日：2024-04-23
申请号：US17390984
申请日：2021-08-01
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： David Vrane
IPC: G01N15/14 , G01N15/1404 , G01N15/10
CPC classification number: G01N15/1404 , G01N2015/1006 , G01N2015/1409
Abstract: A system, method, and apparatus are provided for cytometer fluidics. In one example, a pressure regulation system is provided for a cytometer fluidics system. The pressure regulation system includes a pressure regulator and a transducer. The pressure regulator pressurizes sheath fluid in a fluid path. The transducer senses a measured pressure in the fluid path independently of the sheath tank. The transducer converts the measured pressure into a voltage. The transducer communicates the voltage to the pressure regulator. The pressure regulator translates the voltage to a regulated pressure that substantially matches the measured pressure. The pressure regulator is the only pressure regulation source in the cytometry fluidics system.
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公开(公告)号：US11913868B2
公开(公告)日：2024-02-27
申请号：US17302651
申请日：2021-05-10
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： Ming Yan , Yung-Chieh Hsieh , David Vrane , Eric Chase
IPC: G01N15/00 , G01J3/00 , G01J1/08 , G01N21/47 , G01N15/14 , G01J3/02 , G01J3/36 , G01N15/10 , G01J3/12
CPC classification number: G01N15/1436 , G01J1/08 , G01J3/021 , G01J3/0208 , G01J3/0218 , G01J3/0256 , G01J3/0291 , G01J3/36 , G01N15/1434 , G01N15/1459 , G01N21/47 , G01J2003/1213 , G01N2015/1006 , G01N2015/145
Abstract: In one embodiment, a flow cytometer is disclosed having a compact light detection module. The compact light detection module includes an image array with a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side. Each of the plurality of filters reflects light to one of the plurality of micro-mirrors and passes light of a differing wavelength range and each of the plurality of micro-mirrors reflects light to one of the plurality of filters, such that incident light into the image array zigzags back and forth between consecutive filters of the plurality of filters and consecutive micro-mirrors of the plurality of micro-mirrors. A radius of curvature of each of the plurality of micro-mirrors images the fiber aperture onto the odd filters and collimates the light beam on the even filters.
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