公开(公告)号：US12209948B2

公开(公告)日：2025-01-28

申请号：US17304843

申请日：2021-06-26

Applicant: CYTEK BIOSCIENCES, INC.

Inventor： Maria Jaimes ,  Zhenyu Zhang ,  Jian Zhou ,  Huimin Gu ,  Qing Shao ,  Ming Yan ,  David Vrane

IPC: G01N15/14 ,  G01N15/00 ,  G01N15/1434 ,  G01N15/01

Abstract: In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a full spectrum flow cytometer with five excitation lasers and five corresponding detection modules. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.

公开(公告)号：US20230405592A1

公开(公告)日：2023-12-21

申请号：US18297615

申请日：2023-04-08

Applicant: Cytek Biosciences, Inc.

Inventor： Kuncheng Wang ,  Huimin Gu ,  Andrew Klyce-Mariscal ,  Wing Lee ,  Ming Yan ,  Glen Krueger ,  David Vrane

IPC: B01L3/00 ,  G01N15/14

CPC classification number: B01L3/502746 ,  G01N15/1434 ,  G01N2015/149 ,  G01N2015/1006

Abstract: A flow cell subassembly includes a carriage assembly coupled to a flow cell body to selectively engage a nozzle with a base of a cuvette. The carriage assembly includes a linear bearing slidingly engaged with the flow cell body, a tiltable carriage plate coupled to the linear bearing, and a nozzle mount coupled to the tiltable carriage plate. The nozzle mount receives a nozzle assembly with the nozzle. Set screws can adjust pitch angle of the tiltable carriage plate with the linear bearing to adjust engagement between the nozzle and cuvette in a first dimension. To adjust engagement between the nozzle and cuvette in a second dimension, axial play in bolts/screws through the tiltable carriage plate into threaded holes of the linear bearing allow for yaw angle adjustments.