公开(公告)号：US20210198162A1

公开(公告)日：2021-07-01

申请号：US17058131

申请日：2019-06-21

Applicant: James Weifu Lee

Inventor： James Weifu Lee

IPC: C05G3/40 ,  C05B17/00 ,  B01J19/10 ,  C09C1/44 ,  C05G3/80

Abstract: The present invention discloses a systematic method for phosphorus sustainability, sand soilization and odor removal utilizing the phosphorus-unlocking and odor removing green chemistry of a surface-oxygenated biochar composition made through ozonization in combination with sonication. Surface-oxygenated biochar compositions and sonication-ozonization methods create advanced hydrophilic biochar materials having higher cation exchange capacity, optimized pH, improved wettability, and toxin free components. These sonicated and ozonized biochar compositions are used as filtration materials for clean water and air, as phosphorus solubilizing reagents to mix with phosphate rock materials to make a slow-releasing phosphate fertilizer, as biochar soil additives to help solubilize phosphorus and reduce phosphorus fertilizer additions required to achieve desired soil phosphorus activity, crop uptake, and yield goals, as sand soilization reagents by utilizing their liquid gel-forming activity in the spaces among sand particles to retain water and nutrients and hold the sand particles together, as plant growth stimulants by using the humic acids-like surface-oxygenated biochar substances at a proper ppm concentration and as carbon sequestration agents to help control climate change for energy and environmental sustainability on Earth.

公开(公告)号：US20170009357A1

公开(公告)日：2017-01-12

申请号：US15202214

申请日：2016-07-05

Applicant: James Weifu Lee

Inventor： James Weifu Lee

IPC: C25B1/06 ,  C23F1/14 ,  C12P19/40 ,  C25B1/10

CPC classification number: C25B1/06 ,  C12P19/32 ,  C12Y306/03014 ,  C23F1/14 ,  C25B1/10 ,  Y02E60/366 ,  Y02P20/129

Abstract: Localized excess protons are created with an open-circuit water electrolysis process using a pair of anode and cathode electrodes for a special excess proton production and proton-utilization system to treat a substrate material plate/film by forming and using an excess protons-substrate-hydroxyl anions capacitor-like system. The technology enables protonation and/or proton-driven oxidation of plate/film and/or membrane materials in a pure water environment. The present invention represents a remarkable clean “green chemistry” technology that does not require the use of any conventional acid chemicals including nitric and sulfuric acids for the said industrial applications. The application of localized excess protons provides a special energy recycling and renewing technology function to extract latent heat including molecular thermal motion energy at ambient temperature for generating local proton motive force (equivalent to Gibbs free energy) to do useful work such as driving ATP synthesis and proton-driven oxidation of certain substrate metal atoms.

Abstract translation: 使用一对阳极和阴极用于特殊的过量质子生成和质子利用系统的开放水电解方法产生局部过量质子，以通过形成和使用过量的质子 - 衬底 - 羟基阴离子电容器系统。 该技术可在纯水环境中使板/膜和/或膜材料进行质子化和/或质子驱动氧化。 本发明代表了非常清洁的“绿色化学”技术，其不需要使用任何常规的酸性化学品，包括用于所述工业应用的硝酸和硫酸。 局部过量质子的应用提供了一种特殊的能量回收和更新技术功能，用于提取包括分子热运动能在环境温度下的潜热，以产生局部质子动力（相当于吉布斯自由能）来进行有用的工作，如驱动ATP合成和 某些底物金属原子的质子驱动氧化。

公开(公告)号：US20140212941A1

公开(公告)日：2014-07-31

申请号：US14245848

申请日：2014-04-04

Applicant: James Weifu Lee

Inventor： James Weifu Lee

IPC: C12P7/16

CPC classification number: C12P7/16 ,  A01H5/10 ,  C10G2300/1014 ,  C10L1/02 ,  Y02E50/10 ,  Y02P30/20 ,  Y02P60/247

Abstract: The present invention provides a biosafety-guarded photobiological butanol production technology based on designer transgenic plants, designer algae, designer blue-green algae (cyanobacteria and oxychlorobacteria), or designer plant cells. The designer photosynthetic organisms are created such that the endogenous photobiological regulation mechanism is tamed, and the reducing power (NADPH) and energy (ATP) acquired from the photosynthetic process are used for synthesis of butanol (CH3CH2CH2CH2OH) directly from carbon dioxide (CO2) and water (H2O). The butanol production methods of the present invention completely eliminate the problem of recalcitrant lignocellulosics by bypassing the bottleneck problem of the biomass technology. The photobiological butanol-production technology of the present invention is expected to have a much higher solar-to-butanol energy-conversion efficiency than the current technology and could also help protect the Earth's environment from the dangerous accumulation of CO2 in the atmosphere.

Abstract translation: 本发明提供了基于设计者转基因植物，设计者藻类，设计者蓝绿藻（蓝细菌和氧氯菌）或设计者植物细胞的生物安全保护的光生物丁醇生产技术。 创建设计者光合生物，使得内源性光生物调节机制被驯化，并且使用从光合过程获得的还原能力（NADPH）和能量（ATP）直接从二氧化碳（CO 2）合成丁醇（CH 3 CH 2 CH 2 CH 2 OH）和 水（H2O）。 本发明的丁醇生产方法通过绕过生物质技术的瓶颈问题完全消除了顽固性木质纤维素的问题。 本发明的光生物丁醇生产技术预期具有比现有技术高得多的太阳能 - 丁醇能量转换效率，并且还可以帮助保护地球环境免受大气中二氧化碳的危险的积累。

公开(公告)号：US08653331B2

公开(公告)日：2014-02-18

申请号：US12648567

申请日：2009-12-29

Applicant: James Weifu Lee

Inventor： James Weifu Lee

IPC: C12N15/63 ,  C12N15/82

CPC classification number: C12N15/8257 ,  C07K14/405

Abstract: A process for enhanced photobiological H2 production using transgenic alga. The process includes inducing exogenous genes in a transgenic alga by manipulating selected environmental factors. In one embodiment inducing production of an exogenous gene uncouples H2 production from existing mechanisms that would downregulate H2 production in the absence of the exogenous gene. In other embodiments inducing an exogenous gene triggers a cascade of metabolic changes that increase H2 production. In some embodiments the transgenic alga are rendered non-regenerative by inducing exogenous transgenes for proton channel polypeptides that are targeted to specific algal membranes.

Abstract translation: 使用转基因藻类增强光生物H2生产的方法。 该方法包括通过操纵选择的环境因子来诱导转基因藻类中的外源基因。 在诱导外源基因的产生的一个实施方案中，在不存在外源基因的情况下，将会下调H2生成的现有机制使H2产生脱离。 诱导外源基因的其它实施方案触发增加H2产生的代谢变化的级联。 在一些实施方案中，转基因藻通过诱导针对特定藻类膜的质子通道多肽的外源转基因而变得不再生。

公开(公告)号：US20110256613A1

公开(公告)日：2011-10-20

申请号：US13093413

申请日：2011-04-25

Applicant: James Weifu Lee

Inventor： James Weifu Lee

IPC: C12N1/13

CPC classification number: C12P3/00 ,  C12N15/79 ,  C12N15/8243 ,  C12N15/8269 ,  C12P39/00

Abstract: A designer proton-channel transgenic alga for photobiological hydrogen production that is specifically designed for production of molecular hydrogen (H2) through photosynthetic water splitting. The designer transgenic alga includes proton-conductive channels that are expressed to produce such uncoupler proteins in an amount sufficient to increase the algal H2 productivity. In one embodiment the designer proton-channel transgene is a nucleic acid construct (300) including a PCR forward primer (302), an externally inducible promoter (304), a transit targeting sequence (306), a designer proton-channel encoding sequence (308), a transcription and translation terminator (310), and a PCR reverse primer (312). In various embodiments, the designer proton-channel transgenic algae are used with a gas-separation system (500) and a gas-products-separation and utilization system (600) for photobiological H2 production.

公开(公告)号：US20110177571A1

公开(公告)日：2011-07-21

申请号：US13075153

申请日：2011-03-29

Applicant: James Weifu Lee

Inventor： James Weifu Lee

IPC: C12P7/16

CPC classification number: C12P7/16 ,  C12N15/1137 ,  C12N2310/11 ,  C12N2310/14 ,  C12P7/04 ,  C12Q1/00 ,  C12Y204/01021 ,  Y02E50/10

Abstract: Designer Calvin-cycle-channeled and photosynthetic NADPH-enhanced pathways, the associated designer genes and designer transgenic photosynthetic organisms for photobiological production of butanol and related higher alcohols from carbon dioxide and water are provided. The butanol and related higher alcohols include 1-butanol, 2-methyl-1-butanol, isobutanol, 3-methyl-1-butanol, 1-hexanol, 1-octanol, 1-pentanol, 1-heptanol, 3-methyl-1-pentanol, 4-methyl-1-hexanol, 5-methyl-1-heptanol, 4-methyl-1-pentanol, 5-methyl-1-hexanol, and 6-methyl-1-heptanol. The designer photosynthetic organisms such as designer transgenic oxyphotobacteria and algae comprise designer Calvin-cycle-channeled and photosynthetic NADPH-enhanced pathway gene(s) and biosafety-guarding technology for enhanced photobiological production of butanol and related higher alcohols from carbon dioxide and water.

Abstract translation: 提供了卡尔文循环通道和光合NADPH增强途径的设计，相关的设计者基因和设计者转基因光合生物用于从二氧化碳和水中光生物生产丁醇和相关的高级醇。 丁醇和相关的高级醇包括1-丁醇，2-甲基-1-丁醇，异丁醇，3-甲基-1-丁醇，1-己醇，1-辛醇，1-戊醇，1-庚醇，3-甲基-1- 戊醇，4-甲基-1-己醇，5-甲基-1-庚醇，4-甲基-1-戊醇，5-甲基-1-己醇和6-甲基-1-庚醇。 设计者光合生物如设计者转基因生物光合细菌和藻类包括设计者卡尔文 - 循环通道和光合NADPH增强的途径基因和用于从二氧化碳和水增强丁醇和相关高级醇的光生物生产的生物安全保护技术。

公开(公告)号：US20110120854A1

公开(公告)日：2011-05-26

申请号：US12975307

申请日：2010-12-21

Applicant: James Weifu Lee

Inventor： James Weifu Lee

IPC: B01D3/00

CPC classification number: B01D1/0035 ,  Y02A20/212 ,  Y02B10/20 ,  Y02E10/36

Abstract: A hybrid photovoltaic panel-interfaced solar-greenhouse distillation technology is provided that is capable of utilizing solar waste heat to perform liquid distillation while co-generating solar electricity. Solar waste heat co-generated at a photovoltaic panel is effectively utilized by in situ distillation liquid as an immediate heat sink in thermo contact with the photovoltaic panel front surface, thus providing beneficial cooling of the photovoltaic panel and co-making of distillation products while generating electricity with significant improvement on total-process solar energy utilization efficiency. Its enabled beneficial utilization of waste heat can provide a series of distillation-related products such as: freshwater, sea salts, distilled water, hot water, hot steam, saline/brine products, and brine photobiological cultures for production of advanced biofuels and bioproducts, in addition to solar electricity.

Abstract translation: 提供了一种混合光伏面板接口的太阳能 - 温室蒸馏技术，其能够利用太阳能废热进行液体蒸馏，同时共同生成太阳能。 在光伏面板上共同生成的太阳能废热通过原位蒸馏液体被有效地利用，作为与光伏面板前表面热接触的直接散热器，从而提供对光伏板的有益冷却和蒸馏产物的共同制造，同时产生 电力总体太阳能利用效率显着提高。 它能够有效利用废热可以提供一系列蒸馏相关产品，如：淡水，海盐，蒸馏水，热水，热蒸汽，盐水/盐水产品和盐水光生物培养物，用于生产先进的生物燃料和生物产品， 除了太阳能电力。

公开(公告)号：US20100330637A1

公开(公告)日：2010-12-30

申请号：US12918784

申请日：2009-02-21

Applicant: James Weifu Lee

Inventor： James Weifu Lee

IPC: C12P7/16 ,  A01H13/00

CPC classification number: C12P7/16 ,  A01H5/10 ,  C10G2300/1014 ,  C10L1/02 ,  Y02E50/10 ,  Y02P30/20 ,  Y02P60/247

Abstract: The present invention provides a biosafety-guarded photobiological butanol production technology based on designer transgenic plants, designer algae, designer blue-green algae (cyanobacteria and oxychlorobacteria), or designer plant cells. The designer photosynthetic organisms are created such that the endogenous photobiological regulation mechanism is tamed, and the reducing power (NADPH) and energy (ATP) acquired from the photosynthetic process are used for synthesis of butanol (CH3CH2CH2CH2OH) directly from carbon dioxide (CO2) and water (H2O). The butanol production methods of the present invention completely eliminate the problem of recalcitrant lignocellulosics by bypassing the bottleneck problem of the biomass technology. The photobiological butanol-production technology of the present invention is expected to have a much higher solar-to-butanol energy-conversion efficiency than the current technology and could also help protect the Earth's environment from the dangerous accumulation of CO2 in the atmosphere.

Abstract translation: 本发明提供了基于设计者转基因植物，设计者藻类，设计者蓝绿藻（蓝细菌和氧氯菌）或设计者植物细胞的生物安全保护的光生物丁醇生产技术。 创建设计者光合生物，使得内源性光生物调节机制被驯化，并且使用从光合过程获得的还原能力（NADPH）和能量（ATP）直接从二氧化碳（CO 2）合成丁醇（CH 3 CH 2 CH 2 CH 2 OH）和 水（H2O）。 本发明的丁醇生产方法通过绕过生物质技术的瓶颈问题完全消除了顽固性木质纤维素的问题。 本发明的光生物丁醇生产技术预期具有比现有技术高得多的太阳能 - 丁醇能量转换效率，并且还可以帮助保护地球环境免受大气中二氧化碳的危险的积累。

公开(公告)号：US10071335B2

公开(公告)日：2018-09-11

申请号：US15228611

申请日：2016-08-04

Applicant: James Weifu Lee

Inventor： James Weifu Lee

IPC: B01D53/02 ,  B01J39/24 ,  B01J39/02 ,  C09C3/06 ,  C09C1/44 ,  C09K17/14 ,  C05F11/00 ,  C02F1/28

CPC classification number: B01D53/02 ,  B01D2253/102 ,  B01J39/02 ,  B01J39/24 ,  C02F1/283 ,  C05F11/00 ,  C09C1/44 ,  C09C3/06 ,  C09K17/04 ,  C09K17/14 ,  Y02E50/343

Abstract: Ozonized biochar compositions and methods for creating advanced hydrophilic biochar materials are provided with higher cation exchange capacity, optimized pH, improved wettability, and free of potential toxic components. These ozonized biochar compositions are used as filtration materials, as biochar soil additives and as carbon sequestration agents to help control climate change for energy and environmental sustainability on Earth.

公开(公告)号：US08753837B2

公开(公告)日：2014-06-17

申请号：US12918811

申请日：2009-02-20

Applicant: James Weifu Lee

Inventor： James Weifu Lee

IPC: C12P1/04 ,  C12P7/06 ,  A61K38/00

CPC classification number: C12P7/065 ,  C12N1/12 ,  C12N15/52 ,  Y02E50/17

Abstract: The present invention provides a photobiological ethanol production and harvesting technology using greenhouse distillation systems with designer photosynthetic organisms, such as designer transgenic oxyphotobacteria. The designer oxyphotobacteria are created such that the endogenous photobiological regulation mechanism is tamed, and the reducing power (NADPH) and energy (ATP) acquired from the photosynthetic process are used for synthesis of ethanol (CH3CH2OH) directly from carbon dioxide (CO2) and water (H2O). The designer use of a pair of NADPH-dependent vs. NAD-dependent glyceraldehyde-3-phosphate dehydrogenases in the pathway designs offers a special cyclic “transhydrogenase” redox-shuttle function to convert NADPH to NADH for enhanced photobiological ethanol production. Through combined use of a designer photosynthetic organism with a greenhouse distillation system, the waste solar heat associated with the photobiological ethanol-production process is utilized in harvesting the produced ethanol. In addition to production and harvesting of ethanol, use of the technology can also produce intermediate metabolites and freshwater from seawater.

Abstract translation: 本发明提供了使用具有设计者光合生物的温室蒸馏系统的光生物乙醇生产和收获技术，例如设计者转基因生物光合细菌。 产生设计者产氧光合细菌，使得内源性光生物调节机制被驯化，并且从光合过程获得的还原能力（NADPH）和能量（ATP）直接用于从二氧化碳（CO 2）和水中合成乙醇（CH 3 CH 2 OH） （H 2 O）。 设计者在途径设计中使用一对NADPH依赖性NAD NAD依赖性甘油醛-3-磷酸脱氢酶，提供了一种特殊的循环“转氢酶”氧化还原穿梭功能，可以将NADPH转化为NADH以增强光生物乙醇生产。 通过将设计者光合生物与温室蒸馏系统的组合使用，与光生物乙醇生产过程相关的废太阳能热量用于收获生产的乙醇。 除了生产和收获乙醇外，该技术的使用也可以从海水中产生中等代谢物和淡水。