Organisms for Photobiological Butanol Production from Carbon Dioxide and Water

    公开(公告)号:US20170268025A1

    公开(公告)日:2017-09-21

    申请号:US15613166

    申请日:2017-06-03

    Inventor: James Weifu Lee

    Abstract: The present invention provides a biosafety-guarded photobiological butanol production technology based on designer transgenic plants, designer algae, designer blue-green algae (cyanobacteria and oxychlorobacteria), or designer plant cells. The designer photosynthetic organisms are created such that the endogenous photobiological regulation mechanism is tamed, and the reducing power (NADPH) and energy (ATP) acquired from the photosynthetic process are used for synthesis of butanol (CH3CH2CH2CH2OH) directly from carbon dioxide (CO2) and water (H2O). The butanol production methods of the present invention completely eliminate the problem of recalcitrant lignocellulosics by bypassing the bottleneck problem of the biomass technology. The photobiological butanol-production technology of the present invention is expected to have a much higher solar-to-butanol energy-conversion efficiency than the current technology and could also help protect the Earth's environment from the dangerous accumulation of CO2 in the atmosphere.

    Photovoltaic panel-interfaced solar-greenhouse distillation systems
    3.
    发明授权
    Photovoltaic panel-interfaced solar-greenhouse distillation systems 有权
    光伏面板接口太阳能 - 温室蒸馏系统

    公开(公告)号:US09259662B2

    公开(公告)日:2016-02-16

    申请号:US13569261

    申请日:2012-08-08

    Inventor: James Weifu Lee

    Abstract: A hybrid photovoltaic panel-interfaced solar-greenhouse distillation technology is disclosed that is capable of utilizing solar waste heat to perform liquid distillation while co-generating solar electricity. Solar waste heat co-generated at a photovoltaic panel is effectively utilized by in situ distillation liquid as an immediate heat sink in thermal contact with the photovoltaic panel, thus providing beneficial cooling of the photovoltaic panel and co-making of distillation products while generating electricity with significant improvement on total-process solar energy utilization efficiency. Use of this invention can provide a series of distillation-related products such as freshwater, distilled water, hot water, hot steam, sea salts, saline/brine products, and/or harvest biofuels and bioproducts such as ethanol from renewable resources while co-generating solar electricity.

    Abstract translation: 公开了一种混合光伏面板接口的太阳能 - 温室蒸馏技术,其能够利用太阳能废热进行液体蒸馏,同时共同产生太阳能。 在光伏面板上共同产生的太阳能废热通过原位蒸馏液体被有效地利用,作为与光伏面板热接触的立即散热器,从而提供有益的光伏面板冷却和蒸馏产物的共同制造,同时发电 总体太阳能利用效率显着提高。 本发明的使用可以提供一系列蒸馏相关产品,例如淡水,蒸馏水,热水,热蒸汽,海盐,盐水/盐水产品,和/或从可再生资源收获生物燃料和生物产品如乙醇, 产生太阳能发电。

    PHOTOVOLTAIC PANEL-INTERFACED SOLAR-GREENHOUSE DISTILLATION SYSTEMS
    4.
    发明申请
    PHOTOVOLTAIC PANEL-INTERFACED SOLAR-GREENHOUSE DISTILLATION SYSTEMS 审中-公开
    光伏面板接口太阳能温室蒸发系统

    公开(公告)号:US20150353379A1

    公开(公告)日:2015-12-10

    申请号:US14832455

    申请日:2015-08-21

    Inventor: James Weifu Lee

    Abstract: A hybrid photovoltaic panel-interfaced distillation with and without a hydrophobic microporous membrane distillation process is provided that is capable of utilizing solar waste heat to perform liquid distillation while co-generating solar electricity. Solar waste heat co-generated at a photovoltaic panel is effectively utilized by in situ distillation liquid as an immediate heat sink in thermo contact with the photovoltaic panel, thus providing beneficial cooling of the photovoltaic panel and co-making of distillation products while generating electricity with significant improvement on total-process solar energy utilization efficiency. Its enabled beneficial utilization of waste heat can provide a series of distillation-related products such as: freshwater, sea salts, distilled water, distilled ethanol, hot water, hot steam, saline/brine products, and brine photobiological cultures for production of advanced biofuels and bioproducts, in addition to solar electricity.

    Abstract translation: 提供具有和不具有疏水性微孔膜蒸馏方法的混合光伏面板接口蒸馏,其能够利用太阳能废热进行液体蒸馏,同时共同产生太阳能。 在光伏面板上共同产生的太阳能废热通过原位蒸馏液体被有效地用作与光伏面板热接触的直接散热器,从而提供有益的光伏面板冷却和蒸馏产物的共同制造,同时发电 总体太阳能利用效率显着提高。 能够有效利用废热可以提供一系列蒸馏相关产品,如:淡水,海盐,蒸馏水,蒸馏乙醇,热水,热蒸汽,盐水/盐水产品和盐水光生物培养物,用于生产先进的生物燃料 和生物产品,除了太阳能发电。

    Photobiological hydrogen production with switchable photosystem-II designer algae
    5.
    发明申请
    Photobiological hydrogen production with switchable photosystem-II designer algae 有权
    光生物氢生产与可切换光系统II设计师藻类

    公开(公告)号:US20100239923A1

    公开(公告)日:2010-09-23

    申请号:US12648567

    申请日:2009-12-29

    Inventor: James Weifu Lee

    CPC classification number: C12N15/8257 C07K14/405

    Abstract: A process for enhanced photobiological H2 production using transgenic alga. The process includes inducing exogenous genes in a transgenic alga by manipulating selected environmental factors. In one embodiment inducing production of an exogenous gene uncouples H2 production from existing mechanisms that would downregulate H2 production in the absence of the exogenous gene. In other embodiments inducing an exogenous gene triggers a cascade of metabolic changes that increase H2 production. In some embodiments the transgenic alga are rendered non-regenerative by inducing exogenous transgenes for proton channel polypeptides that are targeted to specific algal membranes.

    Abstract translation: 使用转基因藻类增强光生物H2生产的方法。 该方法包括通过操纵选择的环境因子来诱导转基因藻类中的外源基因。 在诱导外源基因的产生的一个实施方案中,在不存在外源基因的情况下,将会下调H2生成的现有机制使H2产生脱离。 诱导外源基因的其它实施方案触发增加H2产生的代谢变化的级联。 在一些实施方案中,转基因藻通过诱导针对特定藻类膜的质子通道多肽的外源转基因而变得不再生。

    Organisms for photobiological butanol production from carbon dioxide and water

    公开(公告)号:US10519471B2

    公开(公告)日:2019-12-31

    申请号:US15613166

    申请日:2017-06-03

    Inventor: James Weifu Lee

    Abstract: The present invention provides a biosafety-guarded photobiological butanol production technology based on designer transgenic plants, designer algae, designer blue-green algae (cyanobacteria and oxychlorobacteria), or designer plant cells. The designer photosynthetic organisms are created such that the endogenous photobiological regulation mechanism is tamed, and the reducing power (NADPH) and energy (ATP) acquired from the photosynthetic process are used for synthesis of butanol (CH3CH2CH2CH2OH) directly from carbon dioxide (CO2) and water (H2O). The butanol production methods of the present invention completely eliminate the problem of recalcitrant lignocellulosics by bypassing the bottleneck problem of the biomass technology. The photobiological butanol-production technology of the present invention is expected to have a much higher solar-to-butanol energy-conversion efficiency than the current technology and could also help protect the Earth's environment from the dangerous accumulation of CO2 in the atmosphere.

    OZONIZED BIOCHAR: PHOSPHORUS SUSTAINABILITY AND SAND SOILIZATION

    公开(公告)号:US20190002764A1

    公开(公告)日:2019-01-03

    申请号:US16104892

    申请日:2018-08-18

    Inventor: James Weifu Lee

    Abstract: Surface-oxygenated biochar compositions and sonication-ozonization methods create advanced hydrophilic biochar materials having higher cation exchange capacity, optimized pH, improved wettability, and toxin free components. These sonicated and ozonized biochar compositions are used as filtration materials for clean water and air, as phosphorus solubilizing reagents to mix with phosphate rock materials to make a slow-releasing phosphate fertilizer, as biochar soil additives to help solubilize phosphorus and reduce phosphorus fertilizer additions required to achieve desired soil phosphorus activity, crop uptake, and yield goals, as sand soilization reagents by utilizing their liquid gel-forming activity in the spaces among sand particles to retain water and nutrients and hold the sand particles together, as plant growth stimulants by using the humic acids-like surface-oxygenated biochar substances at a proper ppm concentration and as carbon sequestration agents to help control climate change for energy and environmental sustainability on Earth.

    Photovoltaic panel-interfaced solar-greenhouse distillation systems

    公开(公告)号:US10093552B2

    公开(公告)日:2018-10-09

    申请号:US14832455

    申请日:2015-08-21

    Inventor: James Weifu Lee

    Abstract: A hybrid photovoltaic panel-interfaced distillation with and without a hydrophobic microporous membrane distillation process is provided that is capable of utilizing solar waste heat to perform liquid distillation while co-generating solar electricity. Solar waste heat co-generated at a photovoltaic panel is effectively utilized by in situ distillation liquid as an immediate heat sink in thermo contact with the photovoltaic panel, thus providing beneficial cooling of the photovoltaic panel and co-making of distillation products while generating electricity with significant improvement on total-process solar energy utilization efficiency. Its enabled beneficial utilization of waste heat can provide a series of distillation-related products such as: freshwater, sea salts, distilled water, distilled ethanol, hot water, hot steam, saline/brine products, and brine photobiological cultures for production of advanced biofuels and bioproducts, in addition to solar electricity.

    Designer calvin-cycle-channeled production of butanol and related higher alcohols
    9.
    发明授权
    Designer calvin-cycle-channeled production of butanol and related higher alcohols 有权
    设计师卡尔文循环通道生产丁醇和相关高级醇

    公开(公告)号:US08986963B2

    公开(公告)日:2015-03-24

    申请号:US13075153

    申请日:2011-03-29

    Inventor: James Weifu Lee

    Abstract: Designer Calvin-cycle-channeled and photosynthetic NADPH-enhanced pathways, the associated designer genes and designer transgenic photosynthetic organisms for photobiological production of butanol and related higher alcohols from carbon dioxide and water are provided. The butanol and related higher alcohols include 1-butanol, 2-methyl-1-butanol, isobutanol, 3-methyl-1-butanol, 1-hexanol, 1-octanol, 1-pentanol, 1-heptanol, 3-methyl-1-pentanol, 4-methyl-1-hexanol, 5-methyl-1-heptanol, 4-methyl-1-pentanol, 5-methyl-1-hexanol, and 6-methyl-1-heptanol. The designer photosynthetic organisms such as designer transgenic oxyphotobacteria and algae comprise designer Calvin-cycle-channeled and photosynthetic NADPH-enhanced pathway gene(s) and biosafety-guarding technology for enhanced photobiological production of butanol and related higher alcohols from carbon dioxide and water.

    Abstract translation: 提供了卡尔文循环通道和光合NADPH增强途径的设计,相关的设计者基因和设计者转基因光合生物用于从二氧化碳和水中光生物生产丁醇和相关的高级醇。 丁醇和相关的高级醇包括1-丁醇,2-甲基-1-丁醇,异丁醇,3-甲基-1-丁醇,1-己醇,1-辛醇,1-戊醇,1-庚醇,3-甲基-1- 戊醇,4-甲基-1-己醇,5-甲基-1-庚醇,4-甲基-1-戊醇,5-甲基-1-己醇和6-甲基-1-庚醇。 设计者光合生物如设计者转基因生物光合细菌和藻类包括设计者卡尔文 - 循环通道和光合NADPH增强的途径基因和用于从二氧化碳和水增强丁醇和相关高级醇的光生物生产的生物安全保护技术。

    Designer organisms for photobiological butanol production from carbon dioxide and water
    10.
    发明授权
    Designer organisms for photobiological butanol production from carbon dioxide and water 有权
    用二氧化碳和水分制造光生物丁醇的设计生物

    公开(公告)号:US08735651B2

    公开(公告)日:2014-05-27

    申请号:US12918784

    申请日:2009-02-21

    Inventor: James Weifu Lee

    Abstract: The present invention provides a biosafety-guarded photobiological butanol production technology based on designer transgenic plants, designer algae, designer blue-green algae (cyanobacteria and oxychlorobacteria), or designer plant cells. The designer photosynthetic organisms are created such that the endogenous photobiological regulation mechanism is tamed, and the reducing power (NADPH) and energy (ATP) acquired from the photosynthetic process are used for synthesis of butanol (CH3CH2CH2CH2OH) directly from carbon dioxide (CO2) and water (H2O). The butanol production methods of the present invention completely eliminate the problem of recalcitrant lignocellulosics by bypassing the bottleneck problem of the biomass technology. The photobiological butanol-production technology of the present invention is expected to have a much higher solar-to-butanol energy-conversion efficiency than the current technology and could also help protect the Earth's environment from the dangerous accumulation of CO2 in the atmosphere.

    Abstract translation: 本发明提供了基于设计者转基因植物,设计者藻类,设计者蓝绿藻(蓝细菌和氧氯菌)或设计者植物细胞的生物安全保护的光生物丁醇生产技术。 创建设计者光合生物,使得内源性光生物调节机制被驯化,并且使用从光合过程获得的还原能力(NADPH)和能量(ATP)直接从二氧化碳(CO 2)合成丁醇(CH 3 CH 2 CH 2 CH 2 OH)和 水(H2O)。 本发明的丁醇生产方法通过绕过生物质技术的瓶颈问题完全消除了顽固性木质纤维素的问题。 本发明的光生物丁醇生产技术预期具有比现有技术高得多的太阳能 - 丁醇能量转换效率,并且还可以帮助保护地球环境免受大气中二氧化碳的危险的积累。

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