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    Polymerase compositions, methods of making and using same
    22.
    发明授权
    Polymerase compositions, methods of making and using same 有权

    公开(公告)号：US10676724B2

    公开(公告)日：2020-06-09

    申请号：US16270129

    申请日：2019-02-07

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter Vander Horn ,  Daniel Mazur ,  Theo Nikiforov ,  Mindy Landes ,  Eileen Tozer

    IPC: C12N9/12 ,  C12Q1/6869 ,  C12Q1/686

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME
    23.
    发明申请
    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME 审中-公开

    公开(公告)号：US20200002688A1

    公开(公告)日：2020-01-02

    申请号：US16270129

    申请日：2019-02-07

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter VANDER HORN ,  Daniel Mazur ,  Theo Nikiforov ,  Mindy Landes ,  Eileen Tozer

    IPC: C12N9/12 ,  C12Q1/686 ,  C12Q1/6869

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

    Bi-directional sequencing compositions and methods
    24.
    发明授权
    Bi-directional sequencing compositions and methods 有权

    公开(公告)号：US10508306B2

    公开(公告)日：2019-12-17

    申请号：US16123823

    申请日：2018-09-06

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jason Myers ,  Zhoutao Chen ,  Devin Dressman ,  Theo Nikiforov

    IPC: C12P19/34 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869

    Abstract: In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.

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