公开(公告)号：US06806455B2

公开(公告)日：2004-10-19

申请号：US10240615

申请日：2002-09-30

Applicant: Carlos Zarate ,  Paul Donders ,  Ahmad Yekta ,  Zahra Masoumi ,  Peter Ramm

Inventor： Carlos Zarate ,  Paul Donders ,  Ahmad Yekta ,  Zahra Masoumi ,  Peter Ramm

IPC: H01L2700

CPC classification number: G01N21/6408 ,  G01N2201/0648

Abstract: The present invention relates to an apparatus and method for imaging time resolved fluorescence in biochemical and medical samples. In a primary aspect, the device includes a lens of large aperture, a flash lamp in the illumination path, a fast-acting solid state shutter or a gated detector in the emission path, a device for delivering homogenous monochromatic illumination to a plurality of wells distributed within a microwell plate, a digital camera of high quantum efficiency, and a computer under computer control, the lamp is pulsed at short intervals. The fast-acting emission shutter or gated detector operates to limit exposure of the camera to a period some microseconds after the extinction of each lamp pulse, during which only delayed fluorescence is transmitted to the camera. The invention achieves simultaneous time resolved imaging of a plurality of samples, with high sensitivity and high throughput.

Abstract translation: 本发明涉及用于在生物化学和医学样品中成像时间分辨荧光的装置和方法。 在主要方面，该装置包括大孔径的透镜，照明路径中的闪光灯，发射路径中的快速固态快门或门控检测器，用于将均匀单色照明传送到多个孔的装置 分布在微孔板内，高量子效率的数码相机和计算机在计算机控制下，以短时间间隔脉冲。 快速发射快门或门控检测器操作以将相机的曝光限制在每个灯脉冲消失之后几微秒的时段，在此期间仅将延迟的荧光传输到相机。 本发明实现多个样品的同时时间分辨成像，具有高灵敏度和高产量。

公开(公告)号：US20030160151A1

公开(公告)日：2003-08-28

申请号：US10240615

申请日：2002-09-30

Inventor： Carlos Zarate ,  Paul Donders ,  Ahmad Yekta ,  Zahra Masoumi ,  Peter Ramm

IPC: H01L027/00

CPC classification number: G01N21/6408 ,  G01N2201/0648

Abstract: The present invention relates to an apparatus and method for imaging time resolved fluorescence in biochemical and medical samples. In a primary aspect, the device includes a lens of large aperture, a flash lamp in the illumination path, a fast-acting solid state shutter or a gated detector in the emission path, a device for delivering homogenous monochromatic illumination to a plurality of wells distributed within a microwell plate, a digital camera of high quantum efficiency, and a computer under computer control, the lamp is pulsed at short intervals. The fast-acting emission shutter or gated detector operates to limit exposure of the camera to a period some microseconds after the extinction of each lamp pulse, during which only delayed fluorescence is transmitted to the camera. The invention achieves simultaneous time resolved imaging of a plurality of samples, with high sensitivity and high throughput.

Abstract translation: 本发明涉及用于在生物化学和医学样品中成像时间分辨荧光的装置和方法。 在主要方面，该装置包括大孔径的透镜，照明路径中的闪光灯，发射路径中的快速固态快门或门控检测器，用于将均匀单色照明传送到多个孔的装置 分布在微孔板内，高量子效率的数码相机和计算机在计算机控制下，以短时间间隔脉冲。 快速发射快门或门控检测器操作以将相机的曝光限制在每个灯脉冲消失之后几微秒的时段，在此期间仅将延迟的荧光传输到相机。 本发明实现多个样品的同时时间分辨成像，具有高灵敏度和高产量。

公开(公告)号：US6111249A

公开(公告)日：2000-08-29

申请号：US118405

申请日：1998-07-17

Applicant: William Garner, III

Inventor： William Garner, III

IPC: G01N21/15 ,  G01N21/85 ,  H01J40/14 ,  H01J5/02

CPC classification number: G01N21/8507 ,  G01N33/1886 ,  G01N21/15 ,  G01N2201/0218 ,  G01N2201/0648

Abstract: An apparatus for housing a submergible optical sensor is provided. The apparatus comprises a sensor body having a sensor opening. The sensor body is operable to protect a sensor when submerged in a fluid. A shutter is disposed above the sensor opening. A motor couples to the shutter and can rotate the shutter. A controller couples to the motor and is operable to cause the motor to rotate the shutter such that the sensor opening is exposed when the sensor takes a measurement. The controller is further operable to cause the motor to rotate the shutter such that the shutter covers the sensor opening when the sensor is not taking a measurement.

Abstract translation: 提供一种用于容纳潜水光学传感器的装置。 该装置包括具有传感器开口的传感器主体。 传感器主体可操作以在浸没在流体中时保护传感器。 在传感器开口上方设有快门。 电机耦合到快门并可旋转快门。 控制器耦合到电动机并且可操作地使电动机旋转闸门，使得当传感器进行测量时传感器开口暴露。 控制器还可操作以使电动机旋转快门，使得当传感器未进行测量时，快门覆盖传感器开口。

公开(公告)号：US5396334A

公开(公告)日：1995-03-07

申请号：US983650

申请日：1992-12-01

Applicant: Kenji Sugawara

Inventor： Kenji Sugawara

IPC: H01L21/60 ,  G01N21/88 ,  G01R31/28 ,  G01R31/311 ,  H01L21/66 ,  G01B11/00

CPC classification number: H01L24/85 ,  G01N21/8806 ,  G01R31/2853 ,  G01R31/311 ,  H01L24/48 ,  H01L24/78 ,  G01N2021/887 ,  G01N2201/0648 ,  G01N2201/067 ,  H01L2224/05554 ,  H01L2224/05624 ,  H01L2224/45144 ,  H01L2224/4809 ,  H01L2224/48091 ,  H01L2224/48245 ,  H01L2224/48247 ,  H01L2224/48465 ,  H01L2224/48624 ,  H01L2224/49171 ,  H01L2224/49175 ,  H01L2224/73265 ,  H01L2224/78 ,  H01L2224/85424 ,  H01L2224/859 ,  H01L24/45 ,  H01L2924/00014 ,  H01L2924/01004 ,  H01L2924/01005 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01019 ,  H01L2924/01033 ,  H01L2924/01039 ,  H01L2924/01079 ,  H01L2924/01082 ,  H01L2924/014 ,  H01L2924/3025

Abstract: A bonded wire inspection apparatus for inspecting wires bonded between the pads of a semiconductor chip and the leads of a lead frame including: a vertical illuminator that illuminates an object of inspection from above; an optical assembly that images particular portions of the object such as the ball at one end of a wire and the crescent at another end of the wire, by receiving the light that is emitted from the vertical illuminator and reflected by the object; a camera that photographs the image obtained by the optical assembly; and a diaphragm that is provided beneath the vertical illuminator and electrically changeable in its opening diameter. Thus, by adjusting the opening diameter of the diaphragm, clear images of the object are constantly obtained despite the differences of surface smoothness of the portions to be inspected.

Abstract translation: 一种接合线检查装置，用于检查接合在半导体芯片的焊盘和引线框的引线之间的引线，包括：从上方照射检查对象的垂直照明器; 光学组件，其通过接收从垂直照明器发射并被物体反射的光，对线的一端处的物体（例如球）的特定部分和线的另一端的新月形进行成像; 拍摄由光学组件获得的图像的照相机; 以及设置在垂直照明器下方并且其开口直径可电变化的隔膜。 因此，通过调节隔膜的开口直径，尽管要检查的部分的表面平滑度的差异，仍然始终获得物体的清晰图像。

公开(公告)号：US4863689A

公开(公告)日：1989-09-05

申请号：US126857

申请日：1987-11-30

Applicant: Merlin L. Leong ,  John S. Hayward ,  Glyn R. Fox

Inventor： Merlin L. Leong ,  John S. Hayward ,  Glyn R. Fox

IPC: C12Q1/26 ,  C12Q1/66 ,  G01N21/76 ,  G01N33/58

CPC classification number: C12Q1/66 ,  C12Q1/26 ,  G01N21/76 ,  G01N33/582 ,  G01N2021/1768 ,  G01N21/763 ,  G01N2201/0648 ,  Y10T436/206664

Abstract: Apparatus is provided for the detection of chemiluminescence, e.g., the luminescent assay of an analyte in a sample. Sample, namely the production of a permanent photographic record of such chemiluminescence, e.g., by the use of a POLAROID film. The apparatus includes three interrelated elements. The first element comprises a film holder for holding a photographic film sensitive to a chemiluminescent reaction of the sample, the film holder defining a window in registry with any photographic film which may be held in the film holder. The second element comprises a sample holder for holding the sample in registry with the window and any photographic film which may be held in the film holder, and for allowing movements of the sample relative to any photographic film which may be held in the film holder between (i) a raised position, wherein the sample holder, and thus the sample is in spaced relation to any photographic film which may be held in the film holder and (ii) a lowered position, wherein said sample holder is in sufficiently close proximity to any photogarphic film which may be held in the film holder means to allow the sample held thereby directly to contact any photographic film which may be held in the film holder. The third element comprises a shutter operatively associated with the window of the film holder and movable between a closed position, wherein the shutter obstructs the window so as to prevent the sample holder from moving into the lowered position, and an opened position, wherein the window is unobstructed by the shutter so as to permit the sample holder to move from the raised position to the lowered position. The production of the permanent photographic record begins when the test sample in the container is placed in direct contact with the photographic film. The production of the photographic record is stopped when the test sample in the container is removed out of direct contact with the photographic film and the slidig shutter is closed. Various test results can be observed by varying the time of direct contact of the test sample in the reactant container with the photographic film. This provides maximal analytical sensitivity. During the entire test, exposure to ambient light is prevented.

公开(公告)号：US4791310A

公开(公告)日：1988-12-13

申请号：US914367

申请日：1986-10-02

Applicant: Arnold Honig ,  James E. Smith

Inventor： Arnold Honig ,  James E. Smith

IPC: G01N21/64 ,  G02B21/00 ,  G01N21/31

CPC classification number: G01N21/6408 ,  G01N2021/178 ,  G01N2021/641 ,  G01N2021/6413 ,  G01N2021/6421 ,  G01N21/6458 ,  G01N2201/0648

Abstract: A multi-chronal fluorescence imaging technique for spatial differentiation and correlation of a plurality of separate sample components tagged with site specific dyes is described. The dyes have decay times that are widely separated and vary by approximately a factor of ten from one dye to the next. These are added to the sample and are excited with a short pulse of ultraviolet light. Between the sample and a detector is placed an adjustable shutter or gate which is opened and closed at predetermined intervals so that the detector can see the approximate maximum intensity output of each dye in a shown ordered sequence without significant interference from the other dyes.

Abstract translation: 描述了用于用位点特异性染料标记的多个单独的样品组分的空间分化和相关性的多次荧光成像技术。 染料具有广泛分离的衰变时间，并且从一种染料到下一种染料变化约十倍。 将它们加入到样品中并用短脉冲的紫外线激发。 在样品和检测器之间放置可调节的快门或门，其以预定间隔打开和关闭，使得检测器可以以所示有序序列看到每种染料的近似最大强度输出，而没有来自其它染料的显着干扰。