公开(公告)号：US20050106702A1

公开(公告)日：2005-05-19

申请号：US10712491

申请日：2003-11-13

Applicant: Robin Brigmon ,  Sandra Story ,  Denis Altman ,  Christopher Berry

Inventor： Robin Brigmon ,  Sandra Story ,  Denis Altman ,  Christopher Berry

IPC: B09C1/10 ,  C01C1/00 ,  C12N1/20 ,  C12R1/01 ,  C12R1/05 ,  C12R1/40 ,  C12R1/41 ,  C12R1/425 ,  C12R1/44 ,  C12S1/00

CPC classification number: B09C1/10 ,  C12R1/01 ,  C12R1/05 ,  C12R1/40 ,  C12R1/41 ,  C12R1/425 ,  C12R1/44 ,  G21F9/18 ,  G21F9/28 ,  Y10S435/829 ,  Y10S435/874 ,  Y10S435/877 ,  Y10S435/878 ,  Y10S435/88 ,  Y10S435/882

Abstract: Novel strains of isolated and purified bacteria have been identified which have the ability to degrade petroleum hydrocarbons including a variety of PAHs. Several isolates also exhibit the ability to produce a biosurfactant. The combination of the biosurfactant-producing ability along with the ability to degrade PAHs enhances the efficiency with which PAHs may be degraded. Additionally, the biosurfactant also provides an additional ability to bind heavy metal ions for removal from a soil or aquatic environment.

Abstract translation: 已经鉴定了具有分解和纯化的细菌的新菌株，其具有降解包括各种多环芳烃在内的石油烃的能力。 几种分离物也表现出生产生物表面活性剂的能力。 生物表面活性剂生产能力的组合以及降解PAHs的能力提高了多环芳烃降解的效率。 此外，生物表面活性剂还提供了结合重金属离子以从土壤或水生环境中去除的附加能力。

公开(公告)号：US06869783B1

公开(公告)日：2005-03-22

申请号：US09806876

申请日：1999-10-13

Applicant: Marion Ress-Löschke ,  Thomas Friedrich ,  Bernhard Hauer ,  Ralf Mattes ,  Dirk Engels

Inventor： Marion Ress-Löschke ,  Thomas Friedrich ,  Bernhard Hauer ,  Ralf Mattes ,  Dirk Engels

IPC: C12N15/09 ,  C07H21/00 ,  C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05 ,  C12P7/62

CPC classification number: C07H21/00 ,  C12N9/78 ,  C12P7/42 ,  C12P41/006

Abstract: The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs.The invention additionally relates to a process for preparing chiral carboxylic acids from the racemic nitriles.

Abstract translation: 本发明涉及编码具有腈水解酶活性的多肽的核酸序列，包含核酸序列的核酸构建体以及包含核酸序列或核酸构建体的载体。 本发明还涉及由核酸序列编码的氨基酸序列，以及包含核酸序列，核酸构建体或包含核酸序列或核酸构建体的载体的微生物。本发明还涉及一种方法 用于从外消旋腈制备手性羧酸。

公开(公告)号：US6087152A

公开(公告)日：2000-07-11

申请号：US660645

申请日：1996-06-07

Applicant: Hans-Peter Hohmann ,  Luis Pasamontes ,  Michel Tessier ,  Adolphus van Loon

Inventor： Hans-Peter Hohmann ,  Luis Pasamontes ,  Michel Tessier ,  Adolphus van Loon

IPC: A23K1/16 ,  A23L1/30 ,  C07H21/04 ,  C07K14/195 ,  C12N1/21 ,  C12N15/09 ,  C12N15/52 ,  C12N15/53 ,  C12P7/04 ,  C12P23/00 ,  C12R1/05 ,  C12R1/125 ,  C12R1/19 ,  C12R1/20 ,  C12N1/20

CPC classification number: C12N15/52 ,  C07K14/195 ,  C12P23/00

Abstract: Novel proteins of Flavobacterium sp. R1534 and the DNA sequences which encode these proteins are disclosed which provide an improved biosynthetic pathway from farnesyl pyrophosphate and isopentyl pyrophosphate to various carotenoid precursors and carotenoids, especially .beta.-carotene, lycopene, zeaxanthin and cantaxanthin.

Abstract translation: 黄杆菌新蛋白 公开了编码这些蛋白质的DNA序列，其提供了从法呢基焦磷酸盐和异戊基焦磷酸盐到各种类胡萝卜素前体和类胡萝卜素，特别是β-胡萝卜素，番茄红素，玉米黄质和角质黄素的改进的生物合成途径。

公开(公告)号：US5981257A

公开(公告)日：1999-11-09

申请号：US910856

申请日：1997-08-13

Applicant: Toshiaki Fukui ,  Yoshiharu Doi

Inventor： Toshiaki Fukui ,  Yoshiharu Doi

IPC: C12N15/09 ,  C07H21/04 ,  C08G63/06 ,  C08L101/16 ,  C12N1/21 ,  C12N9/00 ,  C12N9/16 ,  C12N9/88 ,  C12P7/62 ,  C12R1/05 ,  C12N1/20 ,  C12N15/00

CPC classification number: C12N9/93 ,  C12N9/88 ,  C12P7/625

Abstract: The present invention relates to a polyester synthase gene coding for a polypeptide containing the amino acid sequence of SEQ ID NO:2 or a sequence where in the amino acid sequence of SEQ ID NO:2, one or more amino acids are deleted, replaced or added, the polypeptide bringing about polyester synthase activity; a gene expression cassette comprising the polyester synthase gene and either of open reading frames located upstream and downstream of the gene; a recombinant vector comprising the gene expression cassette; a transformant transformed with the recombinant vector; and a process for producing polyester by culturing the transformant in a medium and recovering polyester from the resulting culture.

Abstract translation: 本发明涉及编码含有SEQ ID NO：2的氨基酸序列的多肽的聚酯合成酶基因，或其中在SEQ ID NO：2的氨基酸序列中缺失一个或多个氨基酸，替换或 多肽引起聚酯合酶活性; 一种基因表达盒，其包含聚合酶合酶基因和位于该基因上游和下游的开放阅读框; 包含该基因表达盒的重组载体; 用重组载体转化的转化体; 以及通过在培养基中培养转化体并从所得培养物中回收聚酯来生产聚酯的方法。

公开(公告)号：US5962281A

公开(公告)日：1999-10-05

申请号：US466696

申请日：1995-06-06

Applicant: Johann Then ,  Klaus Bartsch ,  Hans-Matthias Deger ,  Susanne Grabley ,  Rudiger Marquardt

Inventor： Johann Then ,  Klaus Bartsch ,  Hans-Matthias Deger ,  Susanne Grabley ,  Rudiger Marquardt

IPC: C12N15/09 ,  C12N1/20 ,  C12N1/21 ,  C12N9/10 ,  C12N15/00 ,  C12N15/54 ,  C12P13/04 ,  C12P13/06 ,  C12R1/01 ,  C12R1/05 ,  C12R1/185 ,  C12R1/19 ,  C12R1/38 ,  C12R1/425 ,  C12R1/465

CPC classification number: C12P13/06 ,  C12N9/1096 ,  C12P13/04

Abstract: L-tertiary-Leucine and L-phosphinothricine are obtainable by transamination of the corresponding keto acids as a precursor in the presence of amino acids as amino group donors. The reaction is preferably carried out with microorganisms or their transaminases.

Abstract translation: 通过在作为氨基供体的氨基酸存在下作为前体的相应酮酸的氨基转移可获得L-叔亮氨酸和L-膦酰四氢叶酸。 该反应优选用微生物或其转氨酶进行。

公开(公告)号：US5871980A

公开(公告)日：1999-02-16

申请号：US894347

申请日：1997-12-01

Applicant: Linda Anne Naylor ,  John Christopher Wood

Inventor： Linda Anne Naylor ,  John Christopher Wood

IPC: C08G63/06 ,  C12P7/62 ,  C12R1/05 ,  C12P7/64

CPC classification number: C12P7/625 ,  Y10S435/829

Abstract: A process for producing poly-3-hydroxyalkanoate (PHA) by culturing Alcaligenes on a low water-solubility aliphatic carboxylic acid and, or hydrolysable derivative of low solubility in pure water, by fermenting the organism in a growth step on a nutrient medium containing inter alia phosphorus in a quantity corresponding to the intended quantity of bacterial cells to be grown until cell growth stops or slows substantially, then in a PHA accumulation stage fermenting the grown cells by feeding said low-solubility carbon source while monitoring pH and adjusting it by addition of ammonia and/or alkaline alkali metal compound until a design quantity of PHA has been produced, and recovering PHA from the product. Further phosphorus may be fed to the accumulation stage at a rate sufficient to permit some bacterial growth but insufficient to permit growth to the exclusion of PHA accumulation. The over-all carbon (as C) to phosphorus (as P) weight ratio is typically in the range 40-100 in the growth stage and 300 to 600 in the accumulation stage.

Abstract translation: PCT No.PCT / GB96 / 00293 Sec。 371 1997年12月1日第 102（e）1997年12月1日PCT PCT 1996年2月9日PCT公布。 公开号WO96 / 25509 日期1996年8月22日通过在低水溶性脂肪族羧酸和/或纯水中溶解度低的可水解衍生物，通过在生长步骤中发酵生物体来培养产生嗜碱性杆菌的方法，生产聚-3-羟基链烷酸酯（PHA） 一种营养培养基，其特别是磷，其量相当于要生长直到细胞生长的细菌细胞的预期量的数量，直到细胞生长停止或显着减慢，然后在PHA积累阶段，通过在监测pH值的同时喂养所述低溶解度碳源来发酵生长的细胞 并通过加入氨和/或碱性碱金属化合物进行调整，直到产生PHA的设计量，并从产品中回收PHA。 进一步的磷可以以足以允许一些细菌生长但不足以允许生长排除PHA积累的速率进料到积聚阶段。 所有碳（如C）至磷（P）重量比通常在生长阶段的40-100范围内，在积累阶段通常为300至600。

公开(公告)号：US5733754A

公开(公告)日：1998-03-31

申请号：US615193

申请日：1996-04-08

Applicant: Richard Anthony Wisdom ,  Caroline Susan Lee ,  Paul Maurice Ricaud

Inventor： Richard Anthony Wisdom ,  Caroline Susan Lee ,  Paul Maurice Ricaud

IPC: C12N1/21 ,  C12N9/80 ,  C12P17/12 ,  C12P41/00 ,  C12R1/05 ,  C12P13/04 ,  C12N1/20 ,  C12N9/78 ,  C12N11/00

CPC classification number: C12R1/05 ,  C12N9/80 ,  C12P17/12 ,  C12P41/007

Abstract: An enzyme is provided which has acylase activity capable of hydrolyzing N-acetyl-(R,S)-pipecolic acid stereoselectively to give (S)-pipecolic acid, wherein this activity is greater than that on N-acetyl-S-proline, at pH 7.5, 25.degree. C., and substrate concentration 20 g/l, in 75 mM Tris buffer. The enzyme is obtainable from the species of Alcaligenes denitrificans deposited as NCIMB 40587. A microorganism having therein the enzyme also is provided, as is a process for preparing (S)-pipecolic acid comprising contacting a mixture of enantiomers of an N-acyl-pipe colic acid with the enzyme or microorganism.

Abstract translation: PCT No.PCT / GB94 / 02259 Sec。 371日期：1996年4月8日 102（e）日期1996年4月8日PCT 1994年10月14日PCT PCT。 公开号WO95 / 10604 日期1995年4月20日提供具有能够立体选择性水解N-乙酰基 - （R，S） - 十二酸的酰基转移酶活性的酶，得到（S） - 十二酸，其中该活性大于N-乙酰基 - S-脯氨酸，pH 7.5,25℃，底物浓度20g / l，在75mM Tris缓冲液中。 该酶可从作为NCIMB 40587沉积的嗜碱性杆菌属物种获得。还提供了其中还含有该酶的微生物，制备（S） - 癸二酸的方法包括使N-酰基管的对映异构体的混合物 肠酸与酶或微生物。

公开(公告)号：US5480787A

公开(公告)日：1996-01-02

申请号：US296921

申请日：1994-08-31

Applicant: Satoshi Negishi ,  Seiichi Shirasawa ,  Junko Suzuki ,  Tateo Murui

Inventor： Satoshi Negishi ,  Seiichi Shirasawa ,  Junko Suzuki ,  Tateo Murui

IPC: C12N9/20 ,  C12N11/14 ,  C12P7/62 ,  C12P7/64 ,  C12R1/05

CPC classification number: C12P7/6454 ,  C12N11/14

Abstract: The transesterification method is conducted with a powdery lipase wherein the powdery lipase is dispersed in ester-containing starting materials in the presence or absence of an inert organic solvent, and the diameter of at least 90% of the dispersed lipase particles is kept in the range of 1 to 100 .mu.m in the course of the trans-esterification reaction. A reactivity higher than that realized by the conventional transesterification method with a powdery lipase is obtained and the lipase can be easily recovered and reused, without causing a loss of the lipase activity, lowering of the conversion due to the residence of the substrate on the immobilizing carrier and side reactions caused by water introduced into the reaction system by the immobilizing carrier.

Abstract translation: 酯交换方法用粉状脂肪酶进行，其中在存在或不存在惰性有机溶剂的情况下将粉状脂肪酶分散在含酯原料中，并且至少90％的分散的脂肪酶颗粒的直径保持在 在反式酯化反应过程中为1〜100微米。 获得比通过常规酯交换法用粉末脂肪酶实现的更高的反应性，并且可以容易地回收和再利用脂肪酶，而不会引起脂肪酶活性的丧失，由于底物停留在固定化上的转化率降低 由固定载体引入反应体系的水引起的载体和副反应。

公开(公告)号：US5273895A

公开(公告)日：1993-12-28

申请号：US966705

申请日：1992-10-26

Applicant: Richard F. Rossi ,  Donald L. Heefner ,  Charles M. Zepp

Inventor： Richard F. Rossi ,  Donald L. Heefner ,  Charles M. Zepp

IPC: C12P7/40 ,  C12P41/00 ,  C12R1/01 ,  C12R1/02 ,  C12R1/025 ,  C12R1/05 ,  C12R1/06 ,  C12R1/07 ,  C12R1/13 ,  C12R1/185 ,  C12R1/38 ,  C12R1/425 ,  C12R1/66 ,  C12R1/72 ,  C12R1/85 ,  C12N9/02

CPC classification number: C12P41/002 ,  C12P7/40

Abstract: A process for enantioselectively converting an aldehyde, a bisulfite adduct of an aldehyde or a glycidate to a chiral carboxylic acid is disclosed. The process utilizes a microorganism or an enzyme preparation from a microorganism and is particularly useful for producing NSAIDs of the profen class from readily available precursors. Preferred microorganisms are Gram-negative rod bacteria.

Abstract translation: 公开了将醛，醛或缩水甘油酸的亚硫酸氢盐加合物对映选择性转化成手性羧酸的方法。 该方法利用来自微生物的微生物或酶制剂，并且特别可用于从容易获得的前体产生等级的NSAID。 优选的微生物是革兰氏阴性杆菌。

公开(公告)号：US5138029A

公开(公告)日：1992-08-11

申请号：US725099

申请日：1991-07-03

Applicant: Masaaki Nishioka ,  Hidemitsu Takinishi ,  Shiro Fukuyama ,  Atsuo Nishimura ,  Hiromi Niitsu

Inventor： Masaaki Nishioka ,  Hidemitsu Takinishi ,  Shiro Fukuyama ,  Atsuo Nishimura ,  Hiromi Niitsu

IPC: C12P7/42 ,  C08G63/06 ,  C08G63/08 ,  C12P7/62 ,  C12R1/05

CPC classification number: C12P7/625 ,  C08G63/06 ,  Y10S435/822 ,  Y10S435/829

Abstract: A random copolymer comprising, as repeating units,(i) 20 to 90 mol % of 3-hydroxybutyrate unit (3HB) having the formula (I): ##STR1## (ii) 5 to 70 mol % of 3-hydroxyvalerate unit (3HV) having the formula (II): ##STR2## and (iii) 1 to 15 mol % of 4-hydroxyvalerate unit (4HV) having the formula (III): ##STR3## wherein the total of the units 3HB, 3HV, and 4HV is 100 mol %, and having a weight average molecular weight within the range of from 10,000 to 2,500,000 and a production process thereof.