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    DROPLET-BASED AFFINITY ASSAYS
    31.
    发明申请
    DROPLET-BASED AFFINITY ASSAYS 有权
    基于DROPLET的亲和力测定

    公开(公告)号：US20130252262A1

    公开(公告)日：2013-09-26

    申请号：US13890715

    申请日：2013-05-09

    Applicant: ADVANCED LIQUID LOGIC INC.

    Inventor： Vijay Srinivasan ,  Vamsee K. Pamula ,  Michael G. Pollack ,  Richard B. Fair

    IPC: G01N27/72

    CPC classification number: G01N27/72 ,  G01N27/745 ,  G01N33/54326

    Abstract: A method of detecting a target analyte in a sample, including executing electrowetting-mediated droplet operations and thereby: combining one or more immunoassay reagent droplets comprising magnetically-responsive beads having affinity for the target analyte with one or more sample droplets potentially comprising the target analyte to yield a first combined droplet; beginning with the combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a first washed droplet comprising the washed magnetically responsive beads; and combining the droplet comprising the washed magnetically responsive beads with a droplet comprising a reporter molecule having affinity for target analyte captured on the magnetically-responsive beads to yield a second combined droplet; beginning with the second combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a second washed droplet comprising the washed magnetically responsive beads; detecting a signal from the second washed droplet which corresponds to the presence, absence and/or quantity of the analyte in the sample.

    Abstract translation: 一种检测样品中的目标分析物的方法，包括执行电润湿介导的液滴操作，从而：将包含对靶分析物具有亲和性的磁响应珠粒的一种或多种免疫测定试剂液滴与潜在地包含靶分析物的一个或多个样品液 以产生第一组合液滴; 从组合的液滴开始，实现基于液滴的洗涤方案以洗涤磁响应珠，以产生包含洗涤的磁响应珠的第一洗涤液滴; 以及将包含洗涤的磁响应珠粒的液滴与包含对在磁响应珠上捕获的靶分析物具有亲和力的报道分子的液滴组合以产生第二组合液滴; 从第二组合液滴开始，实现基于液滴的洗涤方案以洗涤磁响应珠，产生包含洗涤的磁响应珠的第二洗涤液滴; 检测来自第二洗涤液滴的信号，其对应于样品中分析物的存在，不存在和/或数量。

    Method of Manipulating a Droplet
    32.
    发明申请
    Method of Manipulating a Droplet 审中-公开
    操纵水滴的方法

    公开(公告)号：US20130233712A1

    公开(公告)日：2013-09-12

    申请号：US13870709

    申请日：2013-04-25

    Applicant: Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan ,  Philip Paik

    Inventor： Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan ,  Philip Paik

    IPC: B01L3/02

    CPC classification number: B01L3/502792 ,  B01L3/0241 ,  B01L3/50273 ,  B01L2200/06 ,  B01L2300/06 ,  B01L2400/0427 ,  B41J2/14

    Abstract: A method of manipulating a droplet comprising providing a substrate comprising a surface; an elongated transport electrode disposed on the substrate surface, the elongated transport electrode having a first and a second end and configured to impart a gradient force to the droplet; and one or more wires for providing power to the transport electrode; and providing power to the one or more wires to effect the gradient force and thereby transport the droplet along the length of the elongated transport electrode from the first end to the second end.

    Abstract translation: 一种操纵液滴的方法，包括提供包括表面的基底; 设置在基板表面上的细长输送电极，细长输送电极具有第一和第二端并且被配置为向液滴施加梯度力; 以及用于向输送电极供电的一根或多根线; 并且向所述一个或多个电线提供电力以实现所述梯度力，从而将所述液滴沿着所述细长输送电极的长度从所述第一端输送到所述第二端。

    Enhancing and/or Maintaining Oil Film Stability in a Droplet Actuator
    33.
    发明申请
    Enhancing and/or Maintaining Oil Film Stability in a Droplet Actuator 审中-公开
    在液滴致动器中增强和/或维持油膜稳定性

    公开(公告)号：US20130233425A1

    公开(公告)日：2013-09-12

    申请号：US13866105

    申请日：2013-04-19

    Applicant: Vijay Srinivasan ,  Vamsee K. Pamula ,  Ramakrishna Sista ,  Prasanna Thwar

    Inventor： Vijay Srinivasan ,  Vamsee K. Pamula ,  Ramakrishna Sista ,  Prasanna Thwar

    IPC: F16L55/00

    CPC classification number: F16L55/00 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502784 ,  B01L2200/143 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2400/0427 ,  B01L2400/086 ,  Y10T137/206

    Abstract: The present invention is directed to modified droplet actuators, fluids and methods for enhancing and/or maintaining oil film stability in a droplet actuator. In an exemplary embodiment, the invention provides a droplet actuator with one or more substrates arranged to form a droplet operations gap comprising gap-facing surfaces; droplet operations electrodes configured to conduct droplet operations in the droplet operations gap; at least one barrier included on at least one of the substrate surfaces and having dimensions selected to: permit droplet transport from atop a first droplet operations electrode to a second droplet operations electrode when the second droplet operations electrode is activated; and prevent movement of a droplet from atop a first droplet operations electrode when the first and second droplet operations electrodes are inactive.

    Abstract translation: 本发明涉及用于在液滴致动器中增强和/或维持油膜稳定性的改进的液滴致动器，流体和方法。 在示例性实施例中，本发明提供了具有一个或多个基板的液滴致动器，其被布置成形成包括间隙面的表面的液滴操作间隙; 液滴操作电极，被配置为在液滴操作间隙中进行液滴操作; 至少一个屏障包括在至少一个基板表面上，并且具有选定的尺寸：当第二液滴操作电极被激活时允许液滴从第一液滴操作电极顶部传输到第二液滴操作电极; 并且当第一和第二液滴操作电极不活动时，防止液滴从第一液滴操作电极的顶部移动。

    Droplet Actuator Assemblies and Methods of Making Same
    34.
    发明申请
    Droplet Actuator Assemblies and Methods of Making Same 有权
    液滴致动器组件及其制作方法

    公开(公告)号：US20130206597A1

    公开(公告)日：2013-08-15

    申请号：US13807812

    申请日：2011-06-24

    Applicant: Tih-Hong Wang ,  George Brackett ,  David Clevenger ,  Donovan E. Bort

    Inventor： Tih-Hong Wang ,  George Brackett ,  David Clevenger ,  Donovan E. Bort

    IPC: C07K1/26

    CPC classification number: B01L3/0241 ,  B01L3/502784 ,  B41J2/14024

    Abstract: The invention provides droplet actuator assemblies and systems and methods of manufacturing the droplet actuator assemblies. In certain embodiments, two-piece enclosures are used to form a droplet actuator assembly that houses a droplet operations substrate. In certain other embodiments, one-piece enclosures are used to form a droplet actuator assembly that houses a droplet operations substrate. In the plastic injection molding process for forming substrates of the droplet actuator assemblies of the present invention may utilize insert molding (or overmolding) processes for forming a gasket in at least one substrate, thereby avoiding the need for providing and installing a separate gasket component. Further, the droplet actuator assemblies may include features that allow ultrasonic welding processes to be used for bonding substrates together. The manufacturing systems of the present invention for fabricating the droplet actuator assemblies may utilize continuous flow reel-to-reel manufacturing processes.

    Abstract translation: 本发明提供了液滴致动器组件以及制造液滴致动器组件的系统和方法。 在某些实施例中，使用两件式外壳来形成容纳液滴操作基板的液滴致动器组件。 在某些其他实施例中，一体式外壳用于形成容纳液滴操作基板的液滴致动器组件。 在用于形成本发明的液滴致动器组件的基材的塑料注射成型工艺中，可利用嵌入成型（或二次成型）方法在至少一个基板中形成垫圈，从而避免提供和安装单独的垫圈部件。 此外，液滴致动器组件可以包括允许超声波焊接工艺用于将衬底粘合在一起的特征。 用于制造液滴致动器组件的本发明的制造系统可以利用连续流动卷对卷制造过程。

    Method of conducting a droplet based enzymatic assay
    38.
    发明授权
    Method of conducting a droplet based enzymatic assay 有权
    进行基于液滴的酶法测定的方法

    公开(公告)号：US08440392B2

    公开(公告)日：2013-05-14

    申请号：US12531844

    申请日：2008-03-23

    Applicant: Vamsee K. Pamula ,  Allen Eckhardt ,  Jeremy Rouse ,  Vijay Srinivasan

    Inventor： Vamsee K. Pamula ,  Allen Eckhardt ,  Jeremy Rouse ,  Vijay Srinivasan

    IPC: C12Q1/00

    CPC classification number: G01N21/6486 ,  B01F13/0809 ,  C07H15/04 ,  C12Q1/34 ,  G01N2333/928 ,  G01N2800/042

    Abstract: A method of conducting a droplet-based enzymatic assay is provided. The method generally makes use of a droplet actuator. A droplet comprising an enzyme of interest is provided on the droplet actuator along with a droplet comprising a substrate which is potentially modified in the presence of the enzyme. The method involves executing droplet operations on the droplet actuator to combine the droplets, thereby yielding an assay droplet, and detecting modification of the substrate by the enzyme in the assay droplet on the droplet actuator. The enzyme of interest may, for example, be a potentially mutated or improperly folded enzyme exhibiting altered enzyme activity as compared to a corresponding normal enzyme.

    Abstract translation: 提供了一种进行基于液滴的酶测定的方法。 该方法通常使用液滴致动器。 包含感兴趣的酶的液滴与包含底物的液滴一起提供在液滴致动器上，所述液滴在酶的存在下可能被修饰。 该方法涉及对液滴致动器执行液滴操作以组合液滴，从而产生测定液滴，并且通过液滴致动器上的测定液滴中的酶检测底物的修饰。 感兴趣的酶可以是例如与相应的正常酶相比显示改变的酶活性的潜在突变或不正确折叠的酶。

    Integrated Droplet Actuator for Gel; Electrophoresis and Molecular Analysis
    39.
    发明申请
    Integrated Droplet Actuator for Gel; Electrophoresis and Molecular Analysis 有权
    集成液滴执行器凝胶; 电泳和分子分析

    公开(公告)号：US20130059366A1

    公开(公告)日：2013-03-07

    申请号：US13508300

    申请日：2010-11-08

    Applicant: Michael G. Pollack ,  Vijay Srinivasan ,  Zhishan Hua ,  Hon Lung Chu ,  Michael Hauser ,  Allen Eckhardt

    Inventor： Michael G. Pollack ,  Vijay Srinivasan ,  Zhishan Hua ,  Hon Lung Chu ,  Michael Hauser ,  Allen Eckhardt

    IPC: C12M1/40

    CPC classification number: G01N27/44743 ,  B01L3/502715 ,  B01L3/502792 ,  B01L7/525 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2400/0406 ,  B01L2400/043 ,  B01L2400/0457 ,  B01L2400/046 ,  G01N27/44791

    Abstract: The invention is directed to a droplet actuator device and methods for integrating gel electrophoresis analysis with pre or post-analytical sample handling as well as other molecular analysis processes. Using digital microfluidics technology, the droplet actuator device and methods of the invention provide the ability to perform gel electrophoresis and liquid handling operations on a single integrated device. The integrated liquid handling operations may be used to prepare and deliver samples to the electrophoresis gel, capture and subsequently process products of the electrophoresis gel or perform additional assays on the same sample materials which are analyzed by gel electrophoresis. In one embodiment, one or more molecular assays, such as nucleic acid (e.g., DNA) quantification by real-time PCR, and one or more sample processing operations such as sample dilution is performed on a droplet actuator integrated with an electrophoresis gel. In one embodiment, an electrophoresis gel may be integrated on the top substrate of the droplet actuator.

    Abstract translation: 本发明涉及液滴致动器装置以及用于将凝胶电泳分析与分析前样品或分析后处理以及其它分子分析方法相集成的方法。 使用数字微流体技术，液滴致动器装置和本发明的方法提供了在单个集成装置上执行凝胶电泳和液体处理操作的能力。 集成的液体处理操作可以用于制备并将样品递送到电泳凝胶，捕获并随后处理电泳凝胶的产物，或在通过凝胶电泳分析的相同样品材料上进行附加测定。 在一个实施方案中，在与电泳凝胶结合的液滴致动器上进行一个或多个分子测定，例如通过实时PCR的核酸（例如DNA）定量和一个或多个样品处理操作，例如样品稀释。 在一个实施方案中，电泳凝胶可以集成在液滴致动器的顶部基底上。

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