公开(公告)号：US09778262B2

公开(公告)日：2017-10-03

申请号：US14693084

申请日：2015-04-22

Applicant: DAKO DENMARK A/S

Inventor： Zhiming Liao ,  Jeanette Musser

IPC: G01N33/574

CPC classification number: G01N33/57434 ,  G01N2333/47 ,  G01N2333/4742 ,  G01N2333/99 ,  G01N2800/52

Abstract: The present invention relates to a composition comprising at least three primary antibodies or fragments thereof, wherein the at least three antibodies or fragments thereof binds specifically to at least three different proteins, and wherein the at least three different proteins are AMCAR, CK 5/6, and HMWC. Methods for using the composition in diagnosis, prognosis, and assessing efficacy of treatment is further included as well as kits comprising said composition, and optionally, instructions of its use.

公开(公告)号：US20170175178A1

公开(公告)日：2017-06-22

申请号：US15383996

申请日：2016-12-19

Applicant: Dako Denmark A/S

Inventor： Jesper Lohse ,  Mike Paw Maischnack Hansen

IPC: C12Q1/68 ,  C12N9/96 ,  C07D493/10 ,  C07D311/82 ,  C07D311/90 ,  C12N9/08 ,  C12Q1/28

CPC classification number: C12Q1/6841 ,  C07D311/82 ,  C07D311/90 ,  C07D493/10 ,  C12N9/0065 ,  C12N9/96 ,  C12Q1/28 ,  G01N33/581

Abstract: Chromogenic conjugates for color-based detection of targets are described. The conjugates comprise a chromogenic moiety such as rhodamine, rhodol or fluorescein. The chromogenic moiety is linked to a peroxidase substrate. The chromogenic conjugates can be used in immunohistochemical analysis and in situ hybridization. The conjugates can be used to detect 1, 2, 3 or more targets in a sample by color.

公开(公告)号：US09229016B2

公开(公告)日：2016-01-05

申请号：US14306606

申请日：2014-06-17

Applicant: DAKO DENMARK A/S

Inventor： Gordon Feingold ,  Marc Key ,  John Favuzzi ,  Rosanne Welcher

IPC: G01N35/00 ,  G01N1/30 ,  G01N1/31

CPC classification number: G01N35/00732 ,  G01N1/30 ,  G01N1/31 ,  G01N1/312 ,  G01N35/00712 ,  G01N35/00871 ,  G01N35/0092 ,  G01N35/0099 ,  G01N2035/00039 ,  G01N2035/00089 ,  G01N2035/00306 ,  G01N2035/00326 ,  G01N2035/00366 ,  G01N2035/00376 ,  G01N2035/00435 ,  G01N2035/00881 ,  G01N2035/00891 ,  G16H50/20 ,  Y02A90/22 ,  Y02A90/26 ,  Y10S901/02 ,  Y10S901/41 ,  Y10T436/11 ,  Y10T436/112499 ,  Y10T436/113332 ,  Y10T436/114165 ,  Y10T436/114998 ,  Y10T436/25 ,  Y10T436/2575

Abstract: A sample processing system that may be automated and methods are disclosed where samples are arranged on a carrier element and a process operation control system automatically processes the samples perhaps robotically with an operationally-influential exteriorly-consequential information monitor or a data capture element. Significant process details as well as operationally-influential exteriorly-consequential information may be monitored and an automatic notice element may cause notification of a person at some display that may be remote. Various people may be notified, such as an administrator, a supplier, or a manufacturer of an opportunity for some action such as reagent reordering or the like. A simulated motion display may be included to “watch” simulated operation in real time or long after completion of the actual processing.

Abstract translation: 公开了一种可以是自动化的样品处理系统和方法，其中样品被布置在载体元件上，并且过程操作控制系统可以机器人地自动处理样品，其具有操作上有影响力的外部信息监视器或数据捕获元件。 可以监视重要的过程细节以及对操作有影响的外部信息的信息，并且自动通知单元可能导致人们在可能是远程的某些显示器处的通知。 可以通知各种人员，例如管理员，供应商或具有诸如试剂重新排序等的某些动作的机会的制造商。 模拟运动显示可以包括在实际处理完成之后实时或长时间“监视”模拟操作。

公开(公告)号：US20150339818A1

公开(公告)日：2015-11-26

申请号：US14817508

申请日：2015-08-04

Applicant: DAKO DENMARK A/S

Inventor： Rohit Jain ,  Joachim Helmut Schmid ,  Mark Varardo ,  Jeffery Caron

IPC: G06T7/00 ,  G06T7/60

CPC classification number: G06T7/0012 ,  G06K9/0014 ,  G06T7/11 ,  G06T7/136 ,  G06T7/194 ,  G06T7/60 ,  G06T2207/10024 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06T2207/30068

Abstract: Methods and systems for image scoring and analysis are provided. Scored and analyzed images may include digital pathology images. Image scoring and analysis methods may include techniques to identify nuclei and determine membrane staining extent through the use of a priori models. Image scoring and analysis methods may include techniques for membrane intensity determination. Images may be scored based on an extent of membrane staining and membrane intensity.

Abstract translation: 提供了图像评分和分析的方法和系统。 得分和分析的图像可以包括数字病理图像。 图像评分和分析方法可以包括通过使用先验模型识别细胞核并确定膜染色程度的技术。 图像评分和分析方法可以包括用于膜强度测定的技术。 可以根据膜染色和膜强度的程度对图像进行评分。

公开(公告)号：US20150079592A1

公开(公告)日：2015-03-19

申请号：US14504898

申请日：2014-10-02

Applicant: DAKO DENMARK A/S

Inventor： Søren Damgaard Larsen ,  Peter Axel Valbjørn

IPC: G01N1/31 ,  G01N35/00

CPC classification number: G01N1/312 ,  G01N1/2813 ,  G01N35/00029 ,  G01N2035/00079 ,  G01N2035/00138

Abstract: A method and an automated apparatus for processing at least one biological sample arranged on a slide. At least one capillary staining module has a slide rack holder configured to detachably hold a slide rack configured to hold slides, and a capillary lid rack holder configured to detachably hold a capillary lid rack configured to hold capillary lids, wherein the slide rack can be removed independently of removing the capillary lid rack. A first fluid container has a first fluid. The apparatus being configured to automatically rotate the one or more slides, and to move the lids towards the slides to automatically form a capillary gap between each slide and each capillary lid, said capillary gap functioning as a capillary chamber; and to supply an amount of the first fluid to the slide.

Abstract translation: 一种用于处理布置在载玻片上的至少一个生物样品的方法和自动化装置。 至少一个毛细管染色模块具有滑动架保持器，其被配置为可拆卸地保持构造成保持滑块的滑动架，以及毛细管盖架架保持器，其构造成可拆卸地保持构造成保持毛细管盖的毛细管盖架，其中可移除滑架 独立于去除毛细管盖架。 第一流体容器具有第一流体。 所述设备被配置为自动旋转所述一个或多个滑块，并且将所述盖子朝向所述滑块移动，以在每个滑块和每个毛细管盖之间自动形成毛细管间隙，所述毛细管间隙用作毛细管腔室; 并且将一定量的第一流体供应到载玻片。

公开(公告)号：US20140286838A1

公开(公告)日：2014-09-25

申请号：US14299178

申请日：2014-06-09

Applicant: DAKO DENMARK A/S

Inventor： Lars Winther ,  Marc Key ,  Kristopher Buchanan ,  John Favuzzi ,  Benno Guggenheimer

IPC: G01N1/31

CPC classification number: G01N35/00732 ,  G01N1/30 ,  G01N1/31 ,  G01N1/312 ,  G01N35/00712 ,  G01N35/00871 ,  G01N35/0092 ,  G01N35/0099 ,  G01N2035/00039 ,  G01N2035/00089 ,  G01N2035/00306 ,  G01N2035/00326 ,  G01N2035/00366 ,  G01N2035/00376 ,  G01N2035/00435 ,  G01N2035/00881 ,  G01N2035/00891 ,  G16H50/20 ,  Y02A90/22 ,  Y02A90/26 ,  Y10S901/02 ,  Y10S901/41 ,  Y10T436/11 ,  Y10T436/112499 ,  Y10T436/113332 ,  Y10T436/114165 ,  Y10T436/114998 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The present invention concerns an apparatus for automatic processing at least one biological sample accommodated on a carrier member, such as slide by applying a predetermined amount of reagents in a predetermined sequence according to a processing protocol, said apparatus comprising; a housing frame; at least one processing section for accommodating at least one slide, the at least one processing section is provided within the housing; a hood cover protecting the at least one processing section in said housing; wherein the hood cover completely encloses the processing section defining an interior space; and wherein the apparatus further comprises climate control device provided to control the environment within the interior space.

Abstract translation: 本发明涉及一种用于根据处理方案以预定顺序施加预定量的试剂来自动处理容纳在载体构件（例如载玻片）上的至少一种生物样品的装置，所述装置包括： 一个框架; 至少一个处理部分，用于容纳至少一个滑块，所述至少一个处理部分设置在所述壳体内; 保护所述壳体中的至少一个处理部分的罩盖; 其中所述罩盖完全包围限定内部空间的所述处理部分; 并且其中所述装置还包括设置用于控制所述内部空间内的环境的气候控制装置。

公开(公告)号：US08645167B2

公开(公告)日：2014-02-04

申请号：US13690032

申请日：2012-11-30

Applicant: DAKO Denmark A/S

Inventor： Laura Chirica ,  Mats Grahn ,  Stephen Jones

IPC: G06Q50/00

CPC classification number: G06Q10/0633 ,  G06F19/00 ,  G06Q10/06 ,  G16H10/40 ,  G16H40/20 ,  G16H40/67

Abstract: A tangible computer-readable storage device storing computer-executable program instructions that generate a user interface for displaying workflow information associated with a tissue specimen in a pathology laboratory. The program instructions may be configured to perform a method including displaying a virtual laboratory component representing a physical pathology laboratory having one or more laboratory stations for processing the tissue specimen, wherein the tissue specimen is processed by the one or more laboratory stations according to a workflow, and displaying a specimen indicator that indicates a current specimen state based on a current relationship of the tissue specimen to the workflow. The method may further include enabling a first active component associated with the virtual laboratory component, wherein the first active component is configured to receive a user selection of a laboratory station and generating a supplemental view component of the selected laboratory station in response to the user selection, wherein the supplemental view provides supplemental information on processing by the selected laboratory station of the tissue specimen.

Abstract translation: 存储计算机可执行程序指令的有形计算机可读存储设备，其生成用于在病理学实验室中显示与组织标本相关联的工作流信息的用户界面。 程序指令可以被配置为执行一种方法，包括显示代表具有用于处理组织样本的一个或多个实验室的物理病理学实验室的虚拟实验室部件，其中根据工作流程由所述一个或多个实验室站处理所述组织标本 并且基于组织样本与工作流的当前关系，显示指示当前样本状态的样本指示符。 该方法还可以包括启用与虚拟实验室组件相关联的第一活动组件，其中第一活动组件被配置为接收实验室的用户选择并响应于用户选择生成所选实验室的补充视图组件 其中补充视图提供了所选实验室对组织标本进行加工的补充信息。

公开(公告)号：US20140004552A1

公开(公告)日：2014-01-02

申请号：US13919959

申请日：2013-06-17

Applicant: DAKO Denmark A/S

Inventor： John Favuzzi ,  Jonathan Sweda ,  Lauren Bland ,  Merrit Martin ,  Robert Lathrop ,  John Van Bosch ,  Kristopher Buchanan

IPC: G01N1/30

CPC classification number: G01N1/30 ,  B01L3/508 ,  G01N1/312 ,  G01N35/1002 ,  G01N35/109 ,  Y10T436/2575

Abstract: The invention concerns reagent delivery system for an apparatus for processing of biological samples arranged on microscope slides, comprising a reagent section having one or more reagent containers; a slide section in which at least one microscope slide is arranged; a probe for dispensing a portion of reagent onto a predetermined microscope slide, and means for handling the probe. The probe comprises a continuous prove tubing element extending through a rigid probe member and connecting the probe tip to a pneumatic pressure regulation device. The reagent containers are adapted for cooperation with the probe tip. In this manner a high though-put and a very low carry over of fluid residues is achieved since there is no assembled parts making up the inside volume of the probe in which the fluid may be retained.

Abstract translation: 本发明涉及用于处理布置在显微镜载片上的生物样品的装置的试剂递送系统，包括具有一个或多个试剂容器的试剂部分; 其中布置有至少一个显微镜载玻片的滑动部分; 用于将一部分试剂分配到预定显微镜载玻片上的探针和用于处理探针的装置。 探针包括连续证明管道元件，其延伸穿过刚性探针构件并将探针尖端连接到气动调压装置。 试剂容器适于与探针尖端配合。 以这种方式，由于没有组装部件构成可保留流体的探针的内部体积，所以实现了高的保持和非常低的流体残余的携带。

公开(公告)号：US20130217108A1

公开(公告)日：2013-08-22

申请号：US13758810

申请日：2013-02-04

Applicant: DAKO DENMARK A/S

Inventor： Marc Key ,  Gordon Feingold ,  Rosanne Welcher

IPC: G01N1/31

CPC classification number: G01N35/00732 ,  G01N1/30 ,  G01N1/31 ,  G01N1/312 ,  G01N35/00712 ,  G01N35/00871 ,  G01N35/0092 ,  G01N35/0099 ,  G01N2035/00039 ,  G01N2035/00089 ,  G01N2035/00306 ,  G01N2035/00326 ,  G01N2035/00366 ,  G01N2035/00376 ,  G01N2035/00435 ,  G01N2035/00881 ,  G01N2035/00891 ,  G16H50/20 ,  Y02A90/22 ,  Y02A90/26 ,  Y10S901/02 ,  Y10S901/41 ,  Y10T436/11 ,  Y10T436/112499 ,  Y10T436/113332 ,  Y10T436/114165 ,  Y10T436/114998 ,  Y10T436/25 ,  Y10T436/2575

Abstract: A sample processing system 101 that may be automated and methods are disclosed where sample(s) 198 are arranged on a carrier element 197 and a process operation control system 171 automatically processes the sample(s) perhaps robotically according to an desired aggregation of event dictated by an input 173. Alteration of an initial aggregated event topology may be accepted while the system is processing an initial aggregation and varied-parameter robotic control simulation functionalities 606 may be accomplished to determine an enhanced sequence for processing. Suggested operator actions may be displayed that might further enhance the scheduling of the altered aggregated event topology together with an automatic operator need prompt 608 that may inform an operator of a need for a particular action in order to accomplish the desired tasks. Reversibility to proposed changes may be made available so that an operator may avoid having to activate proposed changes if they cause a processing result that is not acceptable.

Abstract translation: 公开了可以是自动化的样本处理系统101和方法，其中样本198布置在载体元件197上，并且过程操作控制系统171可以根据事件规定的所需聚合自动地处理样本 可以通过输入173.当系统正在处理初始聚合并且可变参数机器人控制时可以接受初始聚合事件拓扑的改变，可以实现功能606以确定用于处理的增强的序列。 可以显示建议的操作者动作，其可以进一步增强改变的聚合事件拓扑的调度以及可以通知操作者需要特定动作以实现所需任务的自动操作者需求提示608。 提出的改变的可逆性可以提供，以便操作者可以避免在导致不可接受的处理结果时激活所提出的改变。

公开(公告)号：US20190314471A1

公开(公告)日：2019-10-17

申请号：US16455224

申请日：2019-06-27

Applicant: DAKO DENMARK A/S

Inventor： Jørgen Schøller ,  Henrik Pedersen ,  Liselotte Brix

IPC: A61K39/00 ,  C07K14/74 ,  A61K39/12 ,  A61K39/21 ,  A61K47/61 ,  A61K47/64

Abstract: The present invention relates to methods for construction of pharmamers i.e. vaccine components characterized by their multimerization domain and the attached biologically active molecules, and their use in preparation of vaccines that contains the pharmamers alone or in combination with other molecules. The individual molecules of the construct can be bound to each other or the multimerization domain(s) by covalent or non-covalent bonds, directly or via linkers. The invention further relates to the use of such preparations in vaccine settings aimed to function as preventive/prophylactic or therapeutic vaccines in humans and animals.