公开(公告)号：US20240229132A1

公开(公告)日：2024-07-11

申请号：US18480850

申请日：2023-10-04

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Jia Guo ,  Joshua LaBaer

IPC: C12Q1/6874 ,  C12Q1/6876

CPC classification number: C12Q1/6874 ,  C12Q1/6876 ,  C12Q2563/131

Abstract: Provided herein are methods and systems for sensitive and multiplexed in situ analysis of samples such as biological samples using cleavable fluorescent streptavidin. In particular, provided herein are methods for multiplexed single-cell in situ biomolecule profiling in samples, including fixed or fresh tissues, and also allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

公开(公告)号：US11814677B2

公开(公告)日：2023-11-14

申请号：US17138287

申请日：2020-12-30

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Jia Guo ,  Joshua LaBaer

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6876

CPC classification number: C12Q1/6874 ,  C12Q1/6876 ,  C12Q2563/131

Abstract: Provided herein are methods and systems for sensitive and multiplexed in situ analysis of samples such as biological samples using cleavable hapten linked targeting agents and cleavable detectably-labeled hapten-binding agents. In particular, provided herein are methods for multiplexed single-cell in situ biomolecule profiling in samples, including fixed or fresh tissues, and also allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

公开(公告)号：US20220373548A1

公开(公告)日：2022-11-24

申请号：US17771595

申请日：2020-10-23

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Joshua LaBaer ,  Lusheng Song ,  Ji Qiu ,  Yunro Chung

IPC: G01N33/569 ,  G01N33/574 ,  A61P35/00

Abstract: Provided herein are methods, compositions, kits, and systems for detecting Epstein Barr virus infection (EBV) in gastric cancer (GC) patients. In particular, provided herein are methods, composition, kits, and systems for diagnosing and treating EBV-positive gastric cancer (EBV+ GC) in a biological sample of an individual based on the presence and level of antibodies against particular Epstein Barr virus proteins. EBV+ GC is a distinct subtype of gastric cancer and is associated with unique molecular profiles. Also provided herein are methods for providing more personalized therapy for each of these distinct cancer subtypes and methods for determining an Epstein Barr virus-positive gastric cancer antibody signature, and kits comprising components and protocols for performing the methods of this disclosure.

公开(公告)号：US11060133B2

公开(公告)日：2021-07-13

申请号：US16172379

申请日：2018-10-26

Applicant: Arizona Board of Regents on Behalf of Arizona State University ,  Mayo Foundation for Medical Education and Research

Inventor： Joshua LaBaer ,  Vel Murugan ,  Merica Vorachitti ,  Rahul Pannala ,  Douglas Faigel

IPC: C12Q1/6806 ,  C12Q1/6851 ,  C12Q1/689

Abstract: Provided herein are qPCR-based methods for quantitatively detecting and differentiating between live and dead pathogenic bacteria such as carbapenem-resistant Enterobacteriaceae (CRE). Also provided herein are simple, fast, and reliable genomic DNA extraction methods with improved efficiency.

公开(公告)号：US20210198735A1

公开(公告)日：2021-07-01

申请号：US17138287

申请日：2020-12-30

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Jia Guo ,  Joshua LaBaer

IPC: C12Q1/6874 ,  C12Q1/6876

Abstract: Provided herein are methods and systems for sensitive and multiplexed in situ analysis of samples such as biological samples using cleavable hapten linked targeting agents and cleavable detectably-labeled hapten-binding agents. In particular, provided herein are methods for multiplexed single-cell in situ biomolecule profiling in samples, including fixed or fresh tissues, and also allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

公开(公告)号：US10717977B2

公开(公告)日：2020-07-21

申请号：US15904905

申请日：2018-02-26

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Joshua LaBaer

IPC: C12N15/10 ,  G01N33/68 ,  G01N33/53

Abstract: Methods and compositions for multiplexed protein-protein interaction profiling (e.g., immunoprofiling), based on nucleic acid tagging of polypeptides (e.g., by RNA display) are described. In some embodiments the described compositions and methods utilize a library of prey polypeptide targets linked to prey RNAs encoding them, and a population of bait polypeptides, e.g., a mixture of antibodies, that bind to one or more of the prey polypeptide targets and are used to isolate and identify the bound prey polypeptide targets by amplification of their associated prey RNAs and sequencing of the corresponding cDNAs. In other embodiments the prey polypeptide targets are linked to DNA Bar Codes, which serve as unique identifiers of the tagged polypeptide.

公开(公告)号：US10648978B2

公开(公告)日：2020-05-12

申请号：US15892981

申请日：2018-02-09

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY ,  MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH

Inventor： Haoyu Wang ,  Ji Qiu ,  Joshua LaBaer ,  Jonathan Leighton ,  Shabana Pasha

IPC: G01N33/564 ,  G01N33/50 ,  G01N33/52 ,  G01N33/543 ,  C12N15/10 ,  C07K16/18 ,  C07K16/30 ,  C07K16/40

Abstract: Identification of autoantibodies associated with Crohn's disease useful in diagnosis and management using an innovative protein array technology, namely nucleic acid programmable protein arrays (NAPPA) and applications relating thereto. Overall, reactivity of IgG autoantibodies was stronger than that of IgA autoantibodies; however, IgA autoantibodies showed greater differential reactivity between cases and controls. Four IgA autoantibodies against SNRPB, PRPH, PTTG1 and SNAI1 were newly identified with sensitivities above 15% at 95% specificity, among which anti-SNRPB-IgA had the highest sensitivity of 24.0%. Autoantibodies associated with specific disease subtypes were also found.

公开(公告)号：US20200010899A1

公开(公告)日：2020-01-09

申请号：US16532138

申请日：2019-08-05

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Joshua LaBaer ,  Kristin Gillis ,  Garrick Wallstrom ,  Jin Park ,  Vel Murugan ,  Mitch Magee

IPC: C12Q1/6883

Abstract: Disclosed herein are compositions and methods for accurately estimating the absorbed dose of radiation indicated by a subject based on the expression pattern of a panel of radiation-modulated (RM) genes at various time points following exposure of the subject to ionizing radiation.

公开(公告)号：US10351842B2

公开(公告)日：2019-07-16

申请号：US15310868

申请日：2015-05-14

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Joshua LaBaer

IPC: G01N33/53 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C12N11/06 ,  C07K19/00 ,  C12N9/96 ,  C12N15/10 ,  C12N9/10 ,  C12N9/14 ,  C12N9/42 ,  C12P19/02 ,  C12P19/14

Abstract: Nucleic acid-guided ordered protein assembly (NOPA) arrays and methods for their generation and related applications are disclosed herein.

公开(公告)号：US20180320230A1

公开(公告)日：2018-11-08

申请号：US15770323

申请日：2016-10-27

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Joshua LaBaer ,  Karen Anderson ,  Ji Qiu ,  Sri Krishna

IPC: C12Q1/6881 ,  G01N33/569 ,  G01N33/554

CPC classification number: C12Q1/6881 ,  G01N33/554 ,  G01N33/56972

Abstract: Provided herein are methods of classifying antigens and epitopes as being recognized by an individual's cellular immune response. More particularly, provided herein are methods for unbiased determination of which antigens are recognized by a population of T cells.