公开(公告)号：US08115918B2

公开(公告)日：2012-02-14

申请号：US11788920

申请日：2007-04-23

Applicant: James M. Zavislan ,  Roger J. Greenwald

Inventor： James M. Zavislan ,  Roger J. Greenwald

IPC: G01N21/01

CPC classification number: G02B21/0024 ,  A61B10/0096 ,  G01N1/36 ,  G02B21/34

Abstract: Encapsulated tissue is contained in an optically transparent cassette (34). The cassette (34) or an endcap (38) enclosing the cassette is marked with a fiducial (40) indicating and corresponding to the location of the excision on the patient's body. An image, which is preferably a representation of a surface of the tissue specimen and the vertical section(s) area of the tissue internal of the specimen and adjacent to a surface thereof, is obtained by an imaging system (10). The cassette is moved, preferably in a stage (22) which rotates the cassette while translating it, so that the head (12) of the imaging system provides a linear scan in a direction perpendicular to the wall of the cassette (also perpendicular to the surface of the tissue encapsulated in the cassette). The imaging system's display (28) indicates the morphology at and in proximity to the surface of the specimen as well as the location thereof.

Abstract translation: 包封的组织被包含在光学透明的盒（34）中。 包围盒的盒（34）或端盖（38）标记有基准（40），指示并对应于患者身体上的切除位置。 通过成像系统（10）获得优选地是组织样本的表面的表示和样本的内部并且与其表面相邻的垂直部分区域的图像。 盒子优选地移动到使得盒子平移的阶段（22）中，使得成像系统的头部（12）在垂直于盒子的壁的方向（也垂直于 包裹在盒中的组织的表面）。 成像系统的显示器（28）表示在样品表面处和附近的形态及其位置。

公开(公告)号：US07711410B2

公开(公告)日：2010-05-04

申请号：US10836691

申请日：2004-04-30

Applicant: James M. Zavislan ,  Roger J. Greenwald

Inventor： James M. Zavislan ,  Roger J. Greenwald

IPC: A61B6/00

CPC classification number: G02B21/34 ,  A61B5/0066 ,  A61B5/0068 ,  A61B5/0088 ,  A61B5/445 ,  A61B5/6842 ,  A61B5/6843 ,  A61B5/704 ,  G01N2001/282 ,  G02B21/002

Abstract: A system is provided for marking on a recording medium (36), such as a label, the location of imaged tissue with respect to an exposed surface of the tissue (34). Tissue is imaged by a microscope (11) capable of imaging sections of the tissue below the exposed tissue surface through optics. A ring (32) applied to the surface of the tissue stabilizes the tissue to the optics and localizes a portion of the tissue surface through an aperture in the ring. An actuator (38) is connected to both the ring and the microscope for moving the ring to adjust the position of the tissue with respect to the optics, thereby allowing an operator of the system to survey different images of tissue sections with the microscope.

Abstract translation: 提供了一种用于在诸如标签的记录介质（36）上标记相对于组织（34）的暴露表面的成像组织的位置的系统。 通过显微镜（11）对组织进行成像，该显微镜能够通过光学元件在组织表面下方的组织成像。 应用于组织表面的环（32）将组织稳定到光学元件，并通过环中的孔定位组织表面的一部分。 致动器（38）连接到环和显微镜两者以移动环以调节组织相对于光学器件的位置，从而允许系统的操作者用显微镜检测组织切片的不同图像。

公开(公告)号：US07227630B1

公开(公告)日：2007-06-05

申请号：US09786901

申请日：1999-09-13

Applicant: James M. Zavislan ,  Roger J. Greenwald

Inventor： James M. Zavislan ,  Roger J. Greenwald

IPC: G01N21/01

CPC classification number: G02B21/0028

Abstract: In order to determine, rapidly and without the delay required by conventional tissue preparation techniques for pathological examination (freezing, sectioning, staining, etc.), whether, an excision, which may be a biopsy sample, is representative of the morphology of interest of whether an excisional biopsy in which the tissue taken completely removes the abnormality is, in either case, the tissue which is desired to be excisioned, the tissue specimen (18) in encapsulated, preferably as part of the biopsy procedure. The encapsulated tissue is contained in an optically transparent cassette (34). The cassette (34) or an endcap (38) enclosing the cassette is marked with a fiducial (40) indicating and corresponding to the location of the excision on the patient's body. An image, which is preferably a representation of a surface of the tissue specimen and the vertical section(s) area of the tissue internal of the specimen and adjacent to a surface thereof, is obtained by means of an electro-optical imaging system (10), preferably a confocal laser scanning microscope. The cassette is moved, preferably in a stage (22) which rotates the cassette while translating it, so that the head (12) of the confocal microscope (its objective lens) provides a linear scan in a direction perpendicular to the wall of the cassette (also perpendicular to the surface of the tissue encapsulated in the cassette). The display (28) from the microscope indicates the morphology at and in proximity to the surface of the specimen as well as the location thereof. The pathologist and the surgeon is thereby provided with information concerning the specimen and whether the entire abnormality desired to be removed has indeed been removed and/or whether the biopsy is representative of the body region of interest in the case of a biopsy sample.

Abstract translation: 为了快速确定传统的组织制备技术用于病理检查（冷冻，切片，染色等）所需的延迟，切除可能是活组织检查样本的代表性是代表感兴趣的形态 无论是在所有情况下，组织样本（18）被封装，优选地作为活检程序的一部分，是否完全去除组织的切除活检是否在任何情况下都是希望被切除的组织。 封装的组织被包含在光学透明的盒（34）中。 包围盒的盒（34）或端盖（38）标记有基准（40），指示并对应于患者身体上的切除位置。 通过电光成像系统（10）获得优选地是组织样本的表面的图案和样本内部的组织的垂直截面区域 ），优选共焦激光扫描显微镜。 盒子最好在使盒子旋转的阶段（22）中移动，使得共聚焦显微镜（其物镜）的头部（12）在与盒子的壁垂直的方向上提供线性扫描 （也垂直于包封在盒中的组织的表面）。 来自显微镜的显示器（28）表示在样品表面上和附近的形态及其位置。 因此，病理学家和外科医生提供了关于样本的信息，以及是否确实去除了所有需要去除的整个异常和/或在活组织检查样品的情况下活检是否代表感兴趣的身体区域。

公开(公告)号：US07225010B1

公开(公告)日：2007-05-29

申请号：US09658736

申请日：2000-09-11

Applicant: James M. Zavislan

Inventor： James M. Zavislan

IPC: A61B5/00

CPC classification number: A61B5/6842 ,  A61B5/0068 ,  A61B5/061 ,  A61B5/441 ,  A61B5/444 ,  A61B5/445 ,  A61B5/6834 ,  A61B5/6835 ,  A61B5/6843 ,  A61B5/704

Abstract: An improved system for confocal imaging within dermal tissue of a patient is provided which minimizes instability in confocal images by reducing the relative motion of the tissue with respect to the confocal imaging optics of the system. The system includes a mechanism for maintaining an area of skin tissue under stress by application of force at the edges of the area, and an imaging head coupled to this mechanism for imaging the stressed skin. The mechanism includes a mechanical structure, such as a platen, brace, or attachment, which both supports the imaging head of the system and applies stress to a limited surface area of the tissue to minimize skin motion during confocal imaging.

Abstract translation: 提供了一种用于患者真皮组织内的共焦成像的改进系统，其通过减少组织相对于系统的共聚焦成像光学器件的相对运动而使共焦图像中的不稳定性最小化。 该系统包括用于通过在该区域的边缘处施加力来保持应力下的皮肤组织的面积的机构，以及耦合到该机构的用于对应力皮肤成像的成像头。 该机构包括机械结构，例如压板，支架或附件，其均支撑系统的成像头部，并将应力施加到组织的有限表面区域，以使共聚焦成像期间的皮肤运动最小化。

公开(公告)号：US07190990B2

公开(公告)日：2007-03-13

申请号：US10698294

申请日：2003-10-31

Applicant: James M. Zavislan ,  Roger J. Greenwald

Inventor： James M. Zavislan ,  Roger J. Greenwald

IPC: A61B6/00

CPC classification number: G02B26/12 ,  A61B18/20 ,  A61B18/203 ,  A61B90/20 ,  A61B2018/00452 ,  A61B2018/00636 ,  A61B2562/0242 ,  G02B21/0012 ,  G02B21/0028

Abstract: An improved system for cellular surgery which includes a laser for producing a laser beam, and confocal optics for scanning and focusing the laser beam in tissue and generating confocal images of the tissue in accordance with returned light from the tissue. The confocal images are visualized on a display. The system includes a controller for enabling the operator to select one or more cells of the tissue in the displayed confocal images for surgical treatment. The controller operates the laser and confocal optics in a first mode to treat the tissue when the confocal optics focus the laser beam at least one region associated with the selected cells in the tissue, but at all other times operates the laser and confocal optics in a second mode which does not damage the tissue. The treatment may be localized to concentrate the energy of the laser to the region including the selected cell or cells, or the treatment may be non-localized to distribute the energy of the laser to the region which includes the selected cell(s) and also the cells of the tissue surrounding such selected cell(s). In another embodiment, an apparatus is provided having a confocal imaging system, which focuses a first laser beam through confocal optics to tissue and provides confocal images of the tissue, and a treatment system which focuses a second laser beam through the confocal optics coaxial with the first laser beam for treating at one or more selected locations in the imaged tissue.

Abstract translation: 一种用于细胞手术的改进的系统，其包括用于产生激光束的激光器和用于在组织中扫描和聚焦激光束并根据来自组织的返回光产生组织的共聚焦图像的共聚焦光学器件。 共焦图像在显示器上可视化。 该系统包括用于使操作者能够选择所显示的共聚焦图像中的组织的一个或多个细胞用于外科治疗的控制器。 控制器以第一模式操作激光和共聚焦光学器件，以在共焦光学器件将激光束聚焦到与所选择的细胞相关联的至少一个区域在组织中时处理组织，但是在所有其它时间将激光和共聚焦光学器件操作在 不损伤组织的第二模式。 治疗可以被定位以将激光的能量集中到包括所选择的细胞或细胞的区域，或者治疗可以非局部化以将激光的能量分布到包括所选细胞的区域，并且还 围绕这样选择的细胞的组织的细胞。 在另一个实施例中，提供一种具有共焦成像系统的设备，该共焦成像系统将第一激光束通过共聚焦光学器件聚焦到组织并提供组织的共聚焦图像;以及处理系统，其将第二激光束通过与 第一激光束用于在成像组织中的一个或多个选定位置处理。

公开(公告)号：US07110114B2

公开(公告)日：2006-09-19

申请号：US10787473

申请日：2004-02-26

Applicant: Milind Rajadhyaksha ,  James M. Zavislan

Inventor： Milind Rajadhyaksha ,  James M. Zavislan

IPC: G01J4/00

CPC classification number: G01N21/21 ,  G01N21/47 ,  G01N21/49 ,  G01N2021/4735 ,  G01N2021/4792 ,  G02B21/0032 ,  G02B21/0048 ,  G02B21/0068 ,  G02B21/14 ,  G02B21/33 ,  G02B21/36

Abstract: A confocal scanning microscope system (10) using cross polarization effects and an enhancement agent (acetic acid) to enhance confocal microscope reflectance images of the nuclei of BCCs (basal cell carcinomas) and SCCs (squamous call carcinomas) in the confocal reflectance images of excised tumor slices. The confocal scanning microscope system having a laser (11) for generating an illumination beam (12), a polygon mirror (18) for scanning the beam to a tissue sample (22) and for receiving a return beam from the tissue sample and detector (28) for detecting the returned beam to form an image. The system further includes a half-waveplate (13) having a rotatable stage (14) and a quarter-wave plate (21) having a rotatable stage (20) disposed in the optical path of the illumination beam and at least a linear polarizer (24) having a rotatable stage (25) disposed in the optical pat of the returned beam from the tissue sample.

Abstract translation: 使用交叉极化效应的共焦扫描显微镜系统（10）和增强剂（乙酸）以增强切除的共聚焦反射图像中BCC（基底细胞癌）和SCC（鳞状细胞癌）的细胞核的共聚焦显微镜反射图像 肿瘤切片。 具有用于产生照明光束的激光器（11）的共焦扫描显微镜系统，用于将光束扫描到组织样本（22）并用于从组织样本和检测器（22）接收返回光束的多面镜（18） 28），用于检测返回的光束以形成图像。 该系统还包括具有可旋转台（14）和四分之一波片（21）的半波片（13），其具有设置在照明光束的光路中的可旋转台（20）和至少一个线性偏振器 24）具有设置在来自组织样本的返回光束的光学拍摄中的可旋转台（25）。

公开(公告)号：US06856458B2

公开(公告)日：2005-02-15

申请号：US09973109

申请日：2001-10-09

Applicant: Roger J. Greenwald ,  James M. Zavislan

Inventor： Roger J. Greenwald ,  James M. Zavislan

IPC: B01L3/00 ,  G01N1/36 ,  G01N21/03 ,  G02B21/00 ,  G02B21/34 ,  G01N21/01

CPC classification number: G01N21/0303 ,  B01L3/508 ,  B01L2300/043 ,  B01L2300/0609 ,  B01L2300/0654 ,  B01L2300/0672 ,  B01L2300/0822 ,  B01L2400/0683 ,  G01N1/36 ,  G01N2021/0339 ,  G01N2021/0342 ,  G01N2021/0364 ,  G02B21/0028 ,  G02B21/34

Abstract: A tray or holder for tissue specimens, especially of excised tissue, such as biopsied specimens, is used with a confocal imaging system. The tray may be disposable after imaging of the specimen carried therein or may archive the specimen. A window supports the specimen. Clamps mounted inside the tray restrain the tissue. A compliant bag is mounted outside the tray on one side of a window of the tray on which the specimen is disposed. During imaging the specimen is immersed in a liquid contained in the tray having an index of refraction which closely matches the index of refraction of the tissue. The bag also contains an index matching liquid preferably having the same index as the liquid in the tray. Selecting an immersion liquid, which equals the refractive index of the near surface tissue, minimizing wavefront distortion which may result from an effectively corrugated surface of the specimen.

Abstract translation: 用于组织标本的托盘或保持器，特别是切除的组织，例如活检标本，与共聚焦成像系统一起使用。 托盘在其中携带的样本成像后可以是一次性的，或可以归档样品。 一个窗口支撑样本。 安装在托盘内的夹具限制纸巾。 将合适的袋安装在托盘外侧的托盘的一侧，其中放置有试样。 在成像期间，将样品浸入包含在托盘中的液体中，该折射率与组织的折射率非常匹配。 该袋还包含一种优选地具有与托盘中的液体相同的指数的折射率匹配液体。 选择等于近表面组织的折射率的浸液，最小化可能由于样品的有效波纹表面产生的波前畸变。

公开(公告)号：US06684092B2

公开(公告)日：2004-01-27

申请号：US09138115

申请日：1998-08-21

Applicant: James M. Zavislan

Inventor： James M. Zavislan

IPC: A61B600

CPC classification number: A61B5/0059 ,  A61B5/0066 ,  A61B5/0068 ,  G06F19/00 ,  G06F19/321 ,  G06F19/3418 ,  G16H10/40 ,  G16H80/00 ,  Y10S128/92 ,  Y10S128/922

Abstract: In order to facilitate pathological examination of a lesion in in-vivo tissue, a system and method are provided having a computer system in which both a camera for producing a digital macroscopic picture of the lesion and an imager are coupled to the computer system. The imager is responsive to the computer system and has optics for scanning the lesion to generate images representing microscopic sections of the lesion which provide sufficient information for pathological examination of the lesion. The computer system generates location information, referencing the location in the macroscopic picture of the lesion where the lesion was scanned to the images, and stores data in an electronic file structure which contains at least a representation of the images, a representation of the macroscopic picture, and the location information. The file structure may then be sent to another computer system for viewing the images stored in the file structure to facilitate pathological examination of the lesion by persons trained to interpret such images, adding a diagnostic report about the lesion to the data of the file structure, and sending back the file structure to the computer system that originated it.

Abstract translation: 为了促进体内组织中的病变的病理学检查，提供了一种具有计算机系统的系统和方法，其中用于产生损伤的数字宏观图像的照相机和成像器都耦合到计算机系统。 成像仪响应于计算机系统并且具有用于扫描病变的光学器件以产生表示病变的微观切片的图像，其提供用于病变的病理检查的足够信息。 计算机系统生成位置信息，将病变被扫描的病变的宏观图像中的位置引用到图像，并且将数据存储在至少包含图像的表示的电子文件结构中，宏观图像的表示 ，以及位置信息。 然后可以将文件结构发送到另一计算机系统，以查看存储在文件结构中的图像，以便训练有素的人解读此类图像的病人对病变的病理检查，将关于病变的诊断报告添加到文件结构的数据， 并将文件结构发送回发起它的计算机系统。

公开(公告)号：US06194698B1

公开(公告)日：2001-02-27

申请号：US08748562

申请日：1996-11-13

Applicant: James M. Zavislan ,  Scott R. Grodevant

Inventor： James M. Zavislan ,  Scott R. Grodevant

IPC: H01J4014

CPC classification number: G06K7/1092 ,  G06K7/015 ,  G06K7/10722 ,  G06K7/10861 ,  G06K7/14 ,  G07C13/00

Abstract: An electro-optical sensor senses marks on a sheet which travels longitudinally with respect to a printed circuit board, carrying linear arrays of light sources (LEDs) and photodetectors and optics which define zones displaced laterally across the width of a sheet of paper, longitudinal columns of which can contain marks, the presence and absence of which marks is detected by the sensor. The sensor is especially adapted for use in detecting marks which indicate votes on paper ballots in electronic, computerized vote counting apparatus.

Abstract translation: 电光传感器感测相对于印刷电路板纵向移动的片材上的标记，承载光源（LED）和光电检测器和光学器件的线性阵列，所述光学元件和光学器件限定横向跨越纸张宽度的纵向列 其中可以包含标记，传感器检测到哪些标记的存在和不存在。 传感器特别适用于检测在电子计算机投票计数装置中表示投票的标记。

公开(公告)号：US5635726A

公开(公告)日：1997-06-03

申请号：US545298

申请日：1995-10-19

Applicant: James M. Zavislan ,  Scott R. Grodevant

Inventor： James M. Zavislan ,  Scott R. Grodevant

IPC: G06K7/015 ,  G06K7/10 ,  G06K7/14 ,  G07C13/00 ,  H01L31/12 ,  H03F1/34 ,  H03F3/08 ,  G01N21/86

CPC classification number: G06K7/1092 ,  G06K7/015 ,  G06K7/10722 ,  G06K7/10861 ,  G06K7/14 ,  G07C13/00

Abstract: An electro-optical sensor senses marks on a sheet which travels longitudinally with respect to a printed circuit board, carrying linear arrays of light sources (LEDs) and photodetectors and optics which define zones displaced laterally across the width of a sheet of paper, longitudinal columns of which can contain marks, the presence and absence of which marks is detected by the sensor. The sensor is especially adapted for use in detecting marks which indicate votes on paper ballots in electronic, computerized vote counting apparatus.

Abstract translation: 电光传感器感测相对于印刷电路板纵向移动的片材上的标记，承载光源（LED）和光电检测器和光学器件的线性阵列，所述光学元件和光学器件限定横向跨越纸张宽度的纵向列 其中可以包含标记，传感器检测到哪些标记的存在和不存在。 传感器特别适用于检测在电子计算机投票计数装置中表示投票的标记。