公开(公告)号：US20130344553A1

公开(公告)日：2013-12-26

申请号：US13997242

申请日：2011-12-20

Applicant: James Weifu Lee

Inventor： James Weifu Lee

IPC: C12P7/16

CPC classification number: C12P7/16 ,  C12N15/1137 ,  C12N2310/11 ,  C12N2310/14 ,  C12P7/04 ,  C12Y204/01021 ,  Y02E50/10

Abstract: Designer Calvin-cycle-channeled and hydrogenotrophic biofuel-production pathways, the associated designer genes and designer transgenic organisms for autotrophic production of butanol and related higher alcohols from carbon dioxide, hydrogen, and/or water are provided. The butanol and related higher alcohols include 1-butanol, 2-methyl-1-butanol, isobutanol, 3-methyl-1-butanol, 1-hexanol, 1-octanol, 1-pentanol, 1-heptanol, 3-methyl-1-pentanol, 4-methyl-1-hexanol, 5-methyl-1-heptanol, 4-methyl-1-pentanol, 5-methyl-1-hexanol, and 6-methyl-1-heptanol. The designer autotrophic organisms such as designer transgenic oxyphotobacteria and algae comprise designer Calvin-cycle-channeled and hydrogenotrophic pathway gene(s) and biosafety-guarding technology for enhanced autotrophic production of butanol and related higher alcohols from carbon dioxide and water.

Abstract translation: 提供了设计者卡尔文循环通道和氢营养生物燃料生产途径，相关的设计者基因和设计者转基因生物用于从二氧化碳，氢气和/或水自动生产丁醇和相关的高级醇。 丁醇和相关的高级醇包括1-丁醇，2-甲基-1-丁醇，异丁醇，3-甲基-1-丁醇，1-己醇，1-辛醇，1-戊醇，1-庚醇，3-甲基-1- 戊醇，4-甲基-1-己醇，5-甲基-1-庚醇，4-甲基-1-戊醇，5-甲基-1-己醇和6-甲基-1-庚醇。 设计者自养生物如设计者转基因生物光合细菌和藻类包括设计者卡尔文循环通道和氢营养途径基因和生物安全保护技术，用于增强二氧化碳和水中丁醇和相关高级醇的自养生产。

公开(公告)号：US07973214B2

公开(公告)日：2011-07-05

申请号：US11903919

申请日：2007-09-25

Applicant: James Weifu Lee

Inventor： James Weifu Lee

IPC: A01H5/00 ,  C12N15/52 ,  C12N15/82

CPC classification number: C12N15/8243 ,  C12N15/52 ,  C12N15/8245 ,  C12N15/8269 ,  C12P7/065 ,  Y02E50/16 ,  Y02E50/17 ,  Y02P60/247

Abstract: The present invention provides a revolutionary photosynthetic ethanol production technology based on designer transgenic plants, algae, or plant cells. The designer plants, designer algae, and designer plant cells are created such that the endogenous photosynthesis regulation mechanism is tamed, and the reducing power (NADPH) and energy (ATP) acquired from the photosynthetic water splitting and proton gradient-coupled electron transport process are used for immediate synthesis of ethanol (CH3CH2OH) directly from carbon dioxide (CO2) and water (H2O). The ethanol production methods of the present invention completely eliminate the problem of recalcitrant lignocellulosics by bypassing the bottleneck problem of the biomass technology. The photosynthetic ethanol-production technology of the present invention is expected to have a much higher solar-to-ethanol energy-conversion efficiency than the current technology and could also help protect the Earth's environment from the dangerous accumulation of CO2 in the atmosphere.

Abstract translation: 本发明提供了基于设计者转基因植物，藻类或植物细胞的革命性光合乙醇生产技术。 创建设计者植物，设计者藻类和设计者植物细胞，使得内源光合作用调节机制被驯化，并且从光合水分解和质子梯度耦合电子传递过程获得的还原能力（NADPH）和能量（ATP） 用于直接从二氧化碳（CO2）和水（H 2 O）直接合成乙醇（CH 3 CH 2 OH）。 本发明的乙醇生产方法通过绕过生物质技术的瓶颈问题，完全消除了顽固性木质纤维素的问题。 本发明的光合乙醇生产技术预期将比目前的技术具有高得多的太阳能与乙醇的能量转化效率，并且还可以帮助保护地球环境免受大气中二氧化碳的危险的积累。

公开(公告)号：US20100330639A1

公开(公告)日：2010-12-30

申请号：US12918811

申请日：2009-02-20

Applicant: James Weifu Lee

Inventor： James Weifu Lee

IPC: C12P7/06 ,  C12N15/63

CPC classification number: C12P7/065 ,  C12N1/12 ,  C12N15/52 ,  Y02E50/17

Abstract: The present invention provides a photobiological ethanol production and harvesting technology using greenhouse distillation systems with designer photosynthetic organisms, such as designer transgenic oxyphotobacteria. The designer oxyphotobacteria are created such that the endogenous photobiological regulation mechanism is tamed, and the reducing power (NADPH) and energy (ATP) acquired from the photosynthetic process are used for synthesis of ethanol (CH3CH2OH) directly from carbon dioxide (CO2) and water (H2O). The designer use of a pair of NADPH-dependent vs. NAD-dependent glyceralde-hyde-3-phosphate dehydrogenases in the pathway designs offers a special cyclic “transhydrogenase” redox-shuttle function to convert NADPH to NADH for enhanced photobiological ethanol production. Through combined use of a designer photosynthetic organism with a greenhouse distillation system, the waste solar heat associated with the photobiological ethanol-production process is utilized in harvesting the produced ethanol. In addition to production and harvesting of ethanol, use of the technology can also produce intermediate metabolites and freshwater from seawater.

Abstract translation: 本发明提供了使用具有设计者光合生物的温室蒸馏系统的光生物乙醇生产和收获技术，例如设计者转基因生物光合细菌。 产生设计者产氧光合细菌，使得内源性光生物调节机制被驯化，并且从光合过程获得的还原能力（NADPH）和能量（ATP）直接用于从二氧化碳（CO 2）和水中合成乙醇（CH 3 CH 2 OH） （H 2 O）。 设计者在途径设计中使用一对NADPH依赖性NAD NAD依赖性甘油三酯-3-磷酸脱氢酶提供了一种特殊的循环“转氢酶”氧化还原穿梭功能，可将NADPH转化为NADH以增强光生物乙醇生产。 通过将设计者光合生物与温室蒸馏系统的组合使用，与光生物乙醇生产过程相关的废太阳能热量用于收获生产的乙醇。 除了生产和收获乙醇外，该技术的使用也可以从海水中产生中等代谢物和淡水。

公开(公告)号：US07642405B2

公开(公告)日：2010-01-05

申请号：US11748550

申请日：2007-05-15

Applicant: James Weifu Lee

Inventor： James Weifu Lee

IPC: C12N15/63 ,  C12N15/82

CPC classification number: C12N15/8257 ,  C07K14/405

Abstract: A switchable photosystem-II designer algae for photobiological hydrogen production. The designer transgenic algae includes at least two transgenes for enhanced photobiological H2 production wherein a first transgene serves as a genetic switch that can controls photosystem II (PSII) oxygen evolution and a second transgene encodes for creation of free proton channels in the algal photosynthetic membrane. In one embodiment, the algae includes a DNA construct having polymerase chain reaction forward primer (302), a inducible promoter (304), a PSII-iRNA sequence (306), a terminator (308), and a PCR reverse primer (310). In other embodiments, the PSII-iRNA sequence (306) is replaced with a CF1-iRNA sequence (312), a streptomycin-production gene (314), a targeting sequence (316) followed by a proton-channel producing gene (318), or a PSII-producing gene (320). In one embodiment, a photo-bioreactor and gas-product separation and utilization system produce photobiological H2 from the switchable PSII designer alga.

Abstract translation: 用于光生物氢生产的可切换光系统II设计者藻类。 设计者转基因藻类包括用于增强的光生物H2生产的至少两种转基因，其中第一转基因用作可以控制光系统II（PSII）氧释放的遗传开关，第二转基因编码在藻类光合膜中产生游离质子通道。 在一个实施方案中，藻类包括具有聚合酶链反应正向引物（302），诱导型启动子（304），PSII-iRNA序列（306），终止子（308）和PCR反向引物（310）的DNA构建体 。 在其它实施方案中，用CF1-iRNA序列（312），链霉素生产基因（314），靶向序列（316），随后是质子通道产生基因（318）替代PSII-iRNA序列（306） ，或产生PSII的基因（320）。 在一个实施方案中，光生物反应器和气体产物分离和利用系统从可切换PSII设计者藻产生光生物H2。

公开(公告)号：US20080127364A1

公开(公告)日：2008-05-29

申请号：US11748550

申请日：2007-05-15

Applicant: James Weifu Lee

Inventor： James Weifu Lee

IPC: A01H1/00

CPC classification number: C12N15/8257 ,  C07K14/405

Abstract: A switchable photosystem-II designer algae for photobiological hydrogen production. The designer transgenic algae includes at least two transgenes for enhanced photobiological H2 production wherein a first transgene serves as a genetic switch that can controls photosystem II (PSII) oxygen evolution and a second transgene encodes for creation of free proton channels in the algal photosynthetic membrane. In one embodiment, the algae includes a DNA construct having polymerase chain reaction forward primer (302), a inducible promoter (304), a PSII-iRNA sequence (306), a terminator (308), and a PCR reverse primer (310). In other embodiments, the PSII-iRNA sequence (306) is replaced with a CF1-iRNA sequence (312), a streptomycin-production gene (314), a targeting sequence (316) followed by a proton-channel producing gene (318), or a PSII-producing gene (320). In one embodiment, a photo-bioreactor and gas-product separation and utilization system produce photobiological H2 from the switchable PSII designer alga.

Abstract translation: 用于光生物氢生产的可切换光系统II设计者藻类。 设计者转基因藻类包括用于增强的光生物H 2 O 2生产的至少两种转基因，其中第一转基因用作可控制光系统II（PSII）氧释放的遗传开关，第二转基因编码产生免费的 藻类光合膜中的质子通道。 在一个实施方案中，藻类包括具有聚合酶链反应正向引物（302），诱导型启动子（304），PSII-iRNA序列（306），终止子（308）和PCR反向引物（310）的DNA构建体 。 在其它实施方案中，PSII-iRNA序列（306）被CF1-1RNA序列（312），链霉素生产基因（314），靶向序列（316），随后是 质子通道产生基因（318）或产生PSII的基因（320）。 在一个实施方案中，光生物反应器和气体产物分离和利用系统从可切换PSII设计者藻产生光生物H 2。

公开(公告)号：US06755956B2

公开(公告)日：2004-06-29

申请号：US09873928

申请日：2001-06-04

Applicant: James Weifu Lee ,  Douglas H. Lowndes ,  Vladimir I. Merkulov ,  Gyula Eres ,  Yayi Wei ,  Elias Greenbaum ,  Ida Lee

Inventor： James Weifu Lee ,  Douglas H. Lowndes ,  Vladimir I. Merkulov ,  Gyula Eres ,  Yayi Wei ,  Elias Greenbaum ,  Ida Lee

IPC: C25D518

CPC classification number: C25D21/12 ,  C01B32/162 ,  C01B32/18 ,  C01B2202/02 ,  C01B2202/06 ,  C25D1/02 ,  C25D5/18 ,  C25D7/00 ,  C25D7/0607 ,  G01Q70/12 ,  Y10S977/762 ,  Y10S977/843 ,  Y10S977/873 ,  Y10S977/876 ,  Y10T428/31504

Abstract: A method is described for catalyst-induced growth of carbon nanotubes, nanofibers, and other nanostructures on the tips of nanowires, cantilevers, conductive micro/nanometer structures, wafers and the like. The method can be used for production of carbon nanotube-anchored cantilevers that can significantly improve the performance of scaning probe microscopy (AFM, EFM etc). The invention can also be used in many other processes of micro and/or nanofabrication with carbon nanotubes/fibers. Key elements of this invention include: (1) Proper selection of a metal catalyst and programmable pulsed electrolytic deposition of the desired specific catalyst precisely at the tip of a substrate, (2) Catalyst-induced growth of carbon nanotubes/fibers at the catalyst-deposited tips, (3) Control of carbon nanotube/fiber growth pattern by manipulation of tip shape and growth conditions, and (4) Automation for mass production.

Abstract translation: 描述了在纳米线，悬臂，导电微/纳米结构，晶片等的尖端上的催化剂诱导的碳纳米管，纳米纤维和其他纳米结构生长的方法。 该方法可用于生产可显着提高扫描显微镜（AFM，EFM等）性能的碳纳米管锚定悬臂梁。 本发明也可以用于许多其它使用碳纳米管/纤维进行微观和/或纳米加工的方法。 本发明的关键要素包括：（1）金属催化剂的正确选择和精确地在基材尖端的所需特定催化剂的可编程脉冲电解沉积，（2）催化剂诱导的催化剂 - 沉积尖端，（3）通过操作尖端形状和生长条件控制碳纳米管/纤维生长模式，（4）大规模生产自动化。